scholarly journals Evidence that histamine and carbachol may open the same ion channels in longitudinal smooth muscle of guinea-pig ileum

1981 ◽  
Vol 320 (1) ◽  
pp. 363-379 ◽  
Author(s):  
T. B. Bolton ◽  
J. P. Clark ◽  
K. Kitamura ◽  
R. J. Lang
Keyword(s):  
Smooth Muscle ◽  
Ion Channels ◽  
Guinea Pig ◽  
Guinea Pig Ileum ◽  
Longitudinal Smooth Muscle

scholarly journals Muscarinic cholinergic stimulation of phosphatidylinositol turnover in the longitudinal smooth muscle of guinea-pig ileum

1976 ◽  
Vol 154 (3) ◽  
pp. 653-657 ◽  
Author(s):  
S S. Jafferji ◽  
R H. Michell
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Phosphatidyl Inositol ◽  
Anticholinergic Drugs ◽  
Muscarinic Agonists ◽  
Guinea Pig Ileum ◽  
High Concentration ◽  
Longitudinal Smooth Muscle ◽  
Muscarinic Cholinergic ◽  
Stimulation Of

1. The metabolism of phosphatidylinositol and phosphatidate was investigated in fragments of longitudinal smooth muscle from guinea-pig ileum incubated with cholinergic and anticholinergic drugs. 2. Incorporation of Pi into these lipids was enhanced by acetylcholine and carbamoylcholine. 3. The receptor responsible for triggering this response was of the muscarinic type, since (a) the response was also produced by the muscarinic agonists acetyl-β-methylcholine, carbamoyl-β-methylcholine and pilocarpine, and (b) the response was prevented by atropine and prophylbenzilylcholine mustard, but not by tubocurarine. 4. Increased phosphatidylinositol labellin was clearly observed within 5 min in tissue treated with a high concentration of carbamoylcholine. 5. Halfmaximal stimulation of phosphatidylinositol labelling occurred at approx. 10 muM-muM-carbamoylcholine. 6. Incubation of muscle fragments with carbamoylcholine provoked a decrease in phosphatidylinositol concentration, as would be expected if phosphatidyl-inositol breakdown is the reaction controlled by agonists. 7. This information all appears consistent with the proposal that phosphatidylinositol breakdown may be a reaction intrinsic to the mechanisms of muscarinic cholinergic receptor systems.

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