scholarly journals Protein fractions from mouse salivary glands affecting epithelial cells in organ culture

1973 ◽  
Vol 234 (2) ◽  
pp. 241-255 ◽  
Author(s):  
Barbara E. C. Banks ◽  
S. J. Walter
Keyword(s):  
Epithelial Cells ◽  
Organ Culture ◽  
Salivary Glands ◽  
Protein Fractions

scholarly journals Differential intracellular efficacies of ciprofloxacin and cefixime against Neisseria gonorrhoeae in human fallopian tube organ culture.

1997 ◽  
Vol 41 (7) ◽  
pp. 1547-1551 ◽  
Author(s):  
J P Phanucharas ◽  
G L Gorby
Keyword(s):  
Epithelial Cells ◽  
Confidence Interval ◽  
Neisseria Gonorrhoeae ◽  
Organ Culture ◽  
Fallopian Tube ◽  
Cytochalasin D ◽  
Human Fallopian Tube ◽  
Culture Model ◽  
Better Than

This study compared the abilities of ciprofloxacin and cefixime to kill intracellular Neisseria gonorrhoeae in a human fallopian tube organ culture assay. When invasion was inhibited by cytochalasin D, 0.996% of the tissue-associated gonococci survived ciprofloxacin exposure compared to 1.70% of gonococci exposed to cefixime (95% confidence interval for the ratio of the means, 0.267 to 1.30), indicating that the two antibiotics did not significantly differ in the ability to kill extracellular attached organisms. In the absence of cytochalasin D, 1.63% survived ciprofloxacin exposure while 9.76% survived cefixime treatment (95% confidence interval for the ratio of the means, 0.067 to 0.418). These results suggest that ciprofloxacin penetrated epithelial cells and killed intracellular gonococci better than did cefixime. Thus, at concentrations achievable in serum, ciprofloxacin was more effective in total gonococcal killing than cefixime in this human fallopian tube organ culture model.

scholarly journals Organ Culture of Salivary Glands of Male Anastrepha suspensa (Diptera: Tephritidae)

1995 ◽  
Vol 78 (3) ◽  
pp. 467 ◽  
Author(s):  
Rejane R. De Moraes ◽  
James L. Nation ◽  
James E. Maruniak
Keyword(s):  
Organ Culture ◽  
Salivary Glands ◽  
Anastrepha Suspensa

scholarly journals Single-Cell RNA-seq Identifies Cell Diversity in Embryonic Salivary Glands

2019 ◽  
Vol 99 (1) ◽  
pp. 69-78 ◽  
Author(s):  
R. Sekiguchi ◽  
D. Martin ◽  
K.M. Yamada ◽  
Keyword(s):  
Epithelial Cells ◽  
Parotid Gland ◽  
Single Cell ◽  
Salivary Glands ◽  
Cell Fate ◽  
Mesenchymal Cell ◽  
Muscle Cells ◽  
Mesenchymal Cells ◽  
Organ Specificity ◽  
Bud Initiation

Branching organs, including the salivary and mammary glands, lung, and kidney, arise as epithelial buds that are morphologically very similar. However, the mesenchyme is known to guide epithelial morphogenesis and to help govern cell fate and eventual organ specificity. We performed single-cell transcriptome analyses of 14,441 cells from embryonic day 12 submandibular and parotid salivary glands to characterize their molecular identities during bud initiation. The mesenchymal cells were considerably more heterogeneous by clustering analysis than the epithelial cells. Nonetheless, distinct clusters were evident among even the epithelial cells, where unique molecular markers separated presumptive bud and duct cells. Mesenchymal cells formed separate, well-defined clusters specific to each gland. Neuronal and muscle cells of the 2 glands in particular showed different markers and localization patterns. Several gland-specific genes were characteristic of different rhombomeres. A muscle cluster was prominent in the parotid, which was not myoepithelial or vascular smooth muscle. Instead, the muscle cluster expressed genes that mediate skeletal muscle differentiation and function. Striated muscle was indeed found later in development surrounding the parotid gland. Distinct spatial localization patterns of neuronal and muscle cells in embryonic stages appear to foreshadow later differences in adult organ function. These findings demonstrate that the establishment of transcriptional identities emerges early in development, primarily in the mesenchyme of developing salivary glands. We present the first comprehensive description of molecular signatures that define specific cellular landmarks for the bud initiation stage, when the neural crest–derived ectomesenchyme predominates in the salivary mesenchyme that immediately surrounds the budding epithelium. We also provide the first transcriptome data for the largely understudied embryonic parotid gland as compared with the submandibular gland, focusing on the mesenchymal cell populations.

Antibodies to Lens Epithelium-Derived Growth Factor (LEDGF) Kill Epithelial Cells of Whole Lenses in Organ Culture

1999 ◽  
Vol 69 (1) ◽  
pp. 139-142 ◽  
Author(s):  
MASAHIKO AYAKI ◽  
TOSHIHARU SUENO ◽  
DHIRENDRA P. SINGH ◽  
LEO T. CHYLACK, JR ◽  
TOSHIMICHI SHINOHARA
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Organ Culture ◽  
Lens Epithelium

Serial cultivation of epithelial cells from human and macaque salivary glands

1991 ◽  
Vol 27 (12) ◽  
pp. 939-948 ◽  
Author(s):  
Linda M. Sabatini ◽  
B. Lynn Allen-Hoffmann ◽  
Thomas F. Warner ◽  
Edwin A. Azen
Keyword(s):  
Epithelial Cells ◽  
Salivary Glands ◽  
Serial Cultivation

Role of Fractalkine in the Pathogenesis of Primary Sjögren Syndrome: Increased Serum Levels of Fractalkine, Its Expression in Labial Salivary Glands, and the Association with Clinical Manifestations

2014 ◽  
Vol 41 (12) ◽  
pp. 2425-2438 ◽  
Author(s):  
Jae Ho Lee ◽  
Seung-Ki Kwok ◽  
Seung Min Jung ◽  
Jennifer Lee ◽  
Jae-Seon Lee ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Confocal Microscopy ◽  
Salivary Glands ◽  
Proinflammatory Cytokines ◽  
Sjögren Syndrome ◽  
Control Group ◽  
Sjogren Syndrome ◽  
Clinical Effects ◽  
Extraglandular Manifestations ◽  
Primary Sjögren Syndrome

Objective.To investigate the expression of fractalkine and identify the clinical effects of fractalkine and its receptor (CX3CR1) in patients with primary Sjögren syndrome (pSS).Methods.Serum fractalkine levels were determined by ELISA. Immunohistochemical staining was done to compare the expression of fractalkine and CX3CR1 between salivary glands (SG) of patients with SS and controls. The cells to be merged with fractalkine were evaluated by confocal microscopy. Type of CX3CR1-expressing cells among infiltrating lymphocytes in SG was analyzed by confocal microscopy. Further, associations among fractalkine, proinflammatory cytokines, and clinical profiles were investigated.Results.Serum fractalkine levels in patients with pSS were higher than those in the control group (p = 0.026). SG expression of fractalkine and its receptor was upregulated in patients with pSS compared to that in the controls by immunohistochemistry. Higher histological grade was associated with more fractalkine-positive cells per total epithelial cells. Epithelial cells were the main fractalkine-expressing cell type in the SG. Serum fractalkine levels were significantly correlated with proinflammatory cytokines levels (interleukin 17: r = 0.685, p = 0.029; tumor necrosis factor-α: r = 0.444, p = 0.003), antinuclear antibody (r = 0.349, p = 0.022), and immunoglobulin G levels (r = 0.325, p = 0.044). Serum fractalkine levels in patients with extraglandular manifestations of pSS were significantly higher than in those without extraglandular manifestations (p = 0.026).Conclusion.Fractalkine and CX3CR1 may play a role in the pathogenesis of pSS, including extraglandular manifestations.

Tumor Necrosis Factor Inhibitors Block Apoptosis of Human Epithelial Cells of the Salivary Glands

2009 ◽  
Vol 1171 (1) ◽  
pp. 407-414 ◽  
Author(s):  
Margherita Sisto ◽  
Massimo D'Amore ◽  
Simone Caprio ◽  
Vincenzo Mitolo ◽  
Pasquale Scagliusi ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Epithelial Cells ◽  
Salivary Glands ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Inhibitors ◽  
Human Epithelial Cells ◽  
Necrosis Factor
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