scholarly journals Direct measurements of membrane potential and membrane resistance of human red cells

1968 ◽  
Vol 195 (3) ◽  
pp. 681-696 ◽  
Author(s):  
U. V. Lassen ◽  
O. Sten-Knudsen
Keyword(s):  
Membrane Potential ◽  
Membrane Resistance ◽  
Red Cells ◽  
Direct Measurements ◽  
Human Red Cells

scholarly journals Membrane Resistance of Human Red Cells

1964 ◽  
Vol 47 (5) ◽  
pp. 827-837 ◽  
Author(s):  
S. L. Johnson ◽  
J. W. Woodbury
Keyword(s):  
Glass Tube ◽  
Membrane Resistance ◽  
Total Cell ◽  
Red Cells ◽  
Cell Resistance ◽  
Small Current ◽  
Micron Diameter ◽  
Specific Membrane ◽  
Human Red Cells

A method has been devised to measure the specific membrane resistance of single human red cells. The cells were sucked into a 3 to 5 micron diameter pore in the end of a glass tube. By passing a small current through the cells, the total cell resistance was measured. The dimensions of the cell were measured optically and the specific membrane resistance was then calculated. Leakage of current between the cell and the walls of the pore was minimized by filling this region with isotonic sucrose. The measured specific membrane resistance values of four human red cells were 6.3, 6.32, 10.0, and 19.7 ohm-cm2.

scholarly journals Anion transport in dog, cat, and human red cells. Effects of varying cell volume and Donnan ratio.

1979 ◽  
Vol 74 (3) ◽  
pp. 319-334 ◽  
Author(s):  
V Castranova ◽  
M J Weise ◽  
J F Hoffman
Keyword(s):  
Membrane Potential ◽  
Cell Volume ◽  
Rate Constant ◽  
Rate Constants ◽  
Anion Transport ◽  
Atp Depletion ◽  
Red Cells ◽  
Cell Swelling ◽  
Equilibrium Ratio ◽  
Human Red Cells

Membrane potential and the rate constants for anion self-exchange in dog, cat, and human red blood cells have been shown to vary with cell volume. For dog and cat red cells, the outward rate constants for SO4 and Cl increase while the inward rate constant for SO4 decreases as cells swell or shrink. These changes coincide with the membrane potential becoming more negative as a result of changes in cell volume. Human red cells exhibit a similar change in the rate constants for SO4 and Cl efflux in response to cell swelling, but shrunken cells exhibit a decreased rate constant for SO4 efflux and a more positive membrane potential. Hyperpolarization of shrunken dog and cat red cells is due to a volume-dependent rate constant for SO4 efflux and a more positive membrane potential. Hyperpolarization of shrunken dog and cat red cells is due to a volume-dependent increase in PNa. If this increase in PNa is prevented by ATP depletion or if the outward Na gradient is removed, the response to shrinking is identical to human red cells. These results suggest that the volume dependence of anion permeability may be secondary to changes in the anion equilibrium ratio which in red cells is reflected by the membrane potential. When the membrane potential and cell volume of human red cells were varied independently by a method involving pretreatment with nystatin, it was found that the rate of anion transport (for SO4 and Cl) does not vary with cell volume but rather with membrane potential (anion equilibrium ratio); that is, the rate constant for anion efflux is decreased and that for influx is increased as the membrane potential becomes more positive (internal anion concentration increases) while the opposite is true with membrane hyperpolarization (a fall in internal anion concentration).

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

Metabolic control of the K+ channel of human red cells

1990 ◽  
Vol 116 (1) ◽  
pp. 19-29 ◽  
Author(s):  
Pedro J. Romero ◽  
Carlos E. Ortíz ◽  
Carmelo Melitto
Keyword(s):  
Metabolic Control ◽  
Red Cells ◽  
K Channel ◽  
Human Red Cells

Interactions between sphered human red cells in tube flow: Technique for measuring the strength of antigen-antibody bonds

1982 ◽  
Vol 23 (2) ◽  
pp. 231-238 ◽  
Author(s):  
Harry L. Goldsmith ◽  
Phil Gold ◽  
Joseph Shuster ◽  
Koichi Takamura
Keyword(s):  
Red Cells ◽  
Tube Flow ◽  
Antigen Antibody ◽  
Human Red Cells

The distribution of intracellular calcium chelator (fura-2) in a population of intact human red cells

1993 ◽  
Vol 1148 (1) ◽  
pp. 152-156 ◽  
Author(s):  
Virgilio L. Lew ◽  
Zipora Etzion ◽  
Robert M. Bookchin ◽  
Rui daCosta ◽  
Heikki Väänänen ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Red Cells ◽  
Fura 2 ◽  
Human Red Cells

scholarly journals HUMAN T CELLS BIND AUTOLOGOUS AND HOMOLOGOUS HUMAN RED CELLS

1974 ◽  
Vol 8 (4) ◽  
pp. 402-402
Author(s):  
Joseph Kaplan ◽  
C S Stulberg
Keyword(s):  
T Cells ◽  
Red Cells ◽  
Human T Cells ◽  
Human Red Cells

Caffeine activates a mechanosensitive Ca2+ channel in human red cells

Cell Calcium ◽  
2002 ◽  
Vol 31 (5) ◽  
pp. 189-200 ◽  
Author(s):  
J.F Cordero ◽  
P.J Romero
Keyword(s):  
Red Cells ◽  
Human Red Cells
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