scholarly journals The changes in ionic composition and resting and action potentials in frog sartorius muscle fibres maintainedin vitro

1963 ◽  
Vol 168 (1) ◽  
pp. 1-21 ◽  
Author(s):  
M. H. Draper ◽  
H. Friebel ◽  
K. Karzel
Keyword(s):  
Action Potentials ◽  
Ionic Composition ◽  
Sartorius Muscle ◽  
Muscle Fibres ◽  
Frog Sartorius Muscle ◽  
Frog Sartorius

Qualitative measurements of the entry of L-lactate into single surface fibres of frog skeletal muscle using a lactate-sensitive microelectrode

1987 ◽  
Vol 65 (12) ◽  
pp. 2488-2491 ◽  
Author(s):  
M. J. Mason
Keyword(s):  
Skeletal Muscle ◽  
Lactate Concentration ◽  
Ion Exchanger ◽  
Sartorius Muscle ◽  
Muscle Fibres ◽  
Frog Skeletal Muscle ◽  
Frog Sartorius Muscle ◽  
Frog Sartorius ◽  
Qualitative Measurements ◽  
Liquid Ion Exchanger

The present results demonstrate the sensitivity of the Corning chloride liquid ion exchanger 477913 to L-lactate. Microelectrodes filled with this exchanger showed responses to changes in L-lactate concentration in chloride-free solutions. In these experiments L-lactate replaced gluconate in equimolar amounts. Microelectrodes filled with this exchanger were used to qualitatively detect changes in intracellular anion in chloride-depleted frog sartorius muscle fibres during exposure to extracellular concentrations of L-lactate. The increase in intracellular anion concentration is consistent with the movement of L-lactate into the cell. This microelectrode enables one to qualitatively monitor changes in intracellular L-lactate in chloride-free experiments without incorporating selectivity coefficients.

Constancy of Axial Spacings in Frog Sartorius Muscle during Contraction

Nature ◽  
1965 ◽  
Vol 206 (4991) ◽  
pp. 1358-1358 ◽  
Author(s):  
H. E. HUXLEY ◽  
W. BROWN ◽  
K. C. HOLMES
Keyword(s):  
Sartorius Muscle ◽  
Frog Sartorius Muscle ◽  
Frog Sartorius

Intracellular pH recovery from lactic acidosis of single skeletal muscle fibres

1988 ◽  
Vol 66 (12) ◽  
pp. 1560-1564 ◽  
Author(s):  
Y. E. Allard
Keyword(s):  
Skeletal Muscle ◽  
Lactic Acid ◽  
Intracellular Ph ◽  
Buffer Capacity ◽  
Ringer Solution ◽  
Sartorius Muscle ◽  
Muscle Fibres ◽  
Diffusion Limited ◽  
Skeletal Muscle Fibres ◽  
Frog Sartorius

Intracellular pH (pHi, measured with H+-selective microelectrodes, in quiescent frog sartorius muscle fibres was 7.29 ± 0.09 (n = 13). Frog muscle fibres were superfused with a modified Ringer solution containing 30 mM HEPES buffer, at extracellular pH (pHo) 7.35. Intracellular pH decreased to 6.45 ± 0.14 (n = 13) following replacement of 30 mM NaCl with sodium lactate (30 mM MES, pHo 6.20). Intracellular pH recovery, upon removal of external lactic acid, depended on the buffer concentration of the modified Ringer solution. The measured values of the pHi recovery rates was 0.06 ± 0.01 ΔpHi/min (n = 5) in 3 mM HEPES and was 0.18 ± 0.06 ΔpHi/min (n = 13) in 30 mM HEPES, pHo 7.35. The Na+–H+ exchange inhibitor amiloride (2 mM) slightly reduced pHi recovery rate. The results indicate that the net proton efflux from lactic acidotic frog skeletal muscle is mainly by lactic acid efflux and is limited by the transmembrane pH gradient which, in turn, depends on the extracellular buffer capacity in the diffusion limited space around the muscle fibres.

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