The action of anaesthetic quantities of chloroform upon the blood vessels of the bowel and kidney; with an account of an artificial circulation apparatus

The majority of blood vessels in the superior cervical ganglion possess a continuous endothelium with tight junctions. These same features have been associated with the blood brain barrier of the central nervous system and peripheral nerves. These vessels may perform a barrier function between the capillary circulation and the superior cervical ganglion. The permeability of the blood vessels in the superior cervical ganglion of the rat was tested by intravenous injection of horseradish peroxidase (HRP). Three experimental groups of four animals each were given intravenous HRP (Sigma Type II) in a dosage of.08 to.15 mg/gm body weight in.5 ml of.85% saline. The animals were sacrificed at five, ten or 15 minutes following administration of the tracer. Superior cervical ganglia were quickly removed and fixed by immersion in 2.5% glutaraldehyde in Sorenson's.1M phosphate buffer, pH 7.4. Three control animals received,5ml of saline without HRP. These were sacrificed on the same time schedule. Tissues from experimental and control animals were reacted for peroxidase activity and then processed for routine transmission electron microscopy.

Download Full-text

Permeability of Endometrial Blood Vessels to Exogenous Horse Radish Peroxidase in Mice. Electron Microscope Study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100073258 ◽

1973 ◽

Vol 31 ◽

pp. 562-563

Author(s):

M.C. Castillo-Jessen ◽

A. González-Angulo

Keyword(s):

Electron Microscopy ◽

Blood Vessels ◽

Electron Microscope Study ◽

Ultrastructural Level ◽

Low Molecular Weight ◽

Horse Radish Peroxidase ◽

Intravascular Injection ◽

Normal Morphology ◽

Functional Implications ◽

Low Molecular Weight Proteins

Information regarding the normal morphology of uterine blood vessels at ultrastructural level in mammals is scarce Electron microscopy studies dealing with endometrial vasculature despite the functional implications due to hormone priming are not available. Light microscopy observations with combined injection of dyes and microradiography along with histochemical studies does not enable us to know the detailed fine structure of the possible various types of blood vessels in this tissue. The present work has been designed to characterize the blood vessels of endometrium of mice as well as the behavior of the endothelium to injection of low molecular weight proteins during the normal estrous cycle in this animal. One hundred and forty female albino mice were sacrificed after intravascular injection of horse radish peroxidase (HRP) at 30 seconds, 5, 15, 30 and 60 minutes.

Download Full-text

Some quantitative applications of vascular corrosion casting

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124094 ◽

1992 ◽

Vol 50 (1) ◽

pp. 738-739

Author(s):

Fred E. Hossler

Keyword(s):

Blood Vessels ◽

Nuclear Orientation ◽

Three Dimensional ◽

Surrounding Tissue ◽

Confocal Laser ◽

Corrosion Casting ◽

Venous Valve ◽

Casting Technique ◽

Low Viscosity ◽

Quantitative Measurements

Preparation of replicas of the complex arrangement of blood vessels in various organs and tissues has been accomplished by infusing low viscosity resins into the vasculature. Subsequent removal of the surrounding tissue by maceration leaves a model of the intricate three-dimensional anatomy of the blood vessels of the tissue not obtainable by any other procedure. When applied with care, the vascular corrosion casting technique can reveal fine details of the microvasculature including endothelial nuclear orientation and distribution (Fig. 1), locations of arteriolar sphincters (Fig. 2), venous valve anatomy (Fig. 3), and vessel size, density, and branching patterns. Because casts faithfully replicate tissue vasculature, they can be used for quantitative measurements of that vasculature. The purpose of this report is to summarize and highlight some quantitative applications of vascular corrosion casting. In each example, casts were prepared by infusing Mercox, a methyl-methacrylate resin, and macerating the tissue with 20% KOH. Casts were either mounted for conventional scanning electron microscopy, or sliced for viewing with a confocal laser microscope.

Download Full-text

Cytochemical Specializations on the Luminal Surface of Blood Vessels in the Developing Rat Central Nervous System

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100115936 ◽

1975 ◽

Vol 33 ◽

pp. 462-463

Author(s):

R. S. Hannah ◽

T. H. Rosenquist

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Blood Vessels ◽

Alcian Blue ◽

Luminal Surface ◽

High Voltage Electron Microscopy ◽

Voltage Electron ◽

Concentration Method ◽

Endothelial Surface ◽

Cacodylate Buffer

Developing blood vessels in the rat central nervous system exhibit several unusual luminal features. Hannah (1975) used high voltage electron microscopy to demonstrate numerous ridges of endothelium, some near junctional complexes. The ridges produced troughs (which may appear as depressions) in the endothelial surface. In some areas ridges extended over the troughs, removing them from direct contact with the luminal surface. At no time were the troughs observed to penetrate the basal laminae. Fingerlike projections also extended into the lumina.To determine whether any chemical specializations accompanied the unusual morphological features of the luminal surface, we added 0.1% Alcian blue (Behnke and Zelander, 1970) to the 3% glutaraldehyde perfusate (cacodylate buffer, pH 7.4). After Alcian blue had reacted with the luminal glycocalyces, the dye was dissociated with MgCl2 via critical electrolyte concentration method of Scott and Dorling (1965). When these methods are applied together, it is possible to differentiate mucopolysaccharides (glycosaminoglycans or GAG) with the electron microscope.

Download Full-text