Dysbiosis of the gut microbiome impairs mouse skeletal muscle adaptation to exercise

2021 ◽  
Author(s):  
Taylor R. Valentino ◽  
Ivan J. Vechetti ◽  
C. Brooks Mobley ◽  
Cory M Dungan ◽  
Lesley Golden ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Gut Microbiome ◽  
Muscle Adaptation ◽  
Mouse Skeletal Muscle ◽  
Adaptation To Exercise

scholarly journals Satellite Cell Depletion Disrupts Transcriptional Coordination and Muscle Adaptation to Exercise

Function ◽  
2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Davis A Englund ◽  
Vandré C Figueiredo ◽  
Cory M Dungan ◽  
Kevin A Murach ◽  
Bailey D Peck ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cell ◽  
Satellite Cells ◽  
Gene Networks ◽  
Wheel Running ◽  
Skeletal Muscle Regeneration ◽  
Rna Seq ◽  
Muscle Adaptation ◽  
Hypertrophic Growth ◽  
Adaptation To Exercise

Abstract Satellite cells are required for postnatal development, skeletal muscle regeneration across the lifespan, and skeletal muscle hypertrophy prior to maturity. Our group has aimed to address whether satellite cells are required for hypertrophic growth in mature skeletal muscle. Here, we generated a comprehensive characterization and transcriptome-wide profiling of skeletal muscle during adaptation to exercise in the presence or absence of satellite cells in order to identify distinct phenotypes and gene networks influenced by satellite cell content. We administered vehicle or tamoxifen to adult Pax7-DTA mice and subjected them to progressive weighted wheel running (PoWeR). We then performed immunohistochemical analysis and whole-muscle RNA-seq of vehicle (SC+) and tamoxifen-treated (SC−) mice. Further, we performed single myonuclear RNA-seq to provide detailed information on how satellite cell fusion affects myonuclear transcription. We show that while skeletal muscle can mount a robust hypertrophic response to PoWeR in the absence of satellite cells, growth, and adaptation are ultimately blunted. Transcriptional profiling reveals several gene networks key to muscle adaptation are altered in the absence of satellite cells.

Skeletal Muscle Adaptation to Exercise

Muscle ◽  
2012 ◽  
pp. 911-920 ◽  
Author(s):  
John J. McCarthy ◽  
Karyn A. Esser
Keyword(s):  
Skeletal Muscle ◽  
Muscle Adaptation ◽  
Adaptation To Exercise

scholarly journals Skeletal Muscle Adaptation to Exercise Training

Diabetes ◽  
2007 ◽  
Vol 56 (8) ◽  
pp. 2062-2069 ◽  
Author(s):  
Katja S.C. Röckl ◽  
Michael F. Hirshman ◽  
Josef Brandauer ◽  
Nobuharu Fujii ◽  
Lee A. Witters ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Exercise Training ◽  
Muscle Adaptation ◽  
Adaptation To Exercise

scholarly journals GeneXX: an online tool for the exploration of transcript changes in skeletal muscle associated with exercise

2018 ◽  
Vol 50 (5) ◽  
pp. 376-384 ◽  
Author(s):  
Saskia Reibe ◽  
Marit Hjorth ◽  
Mark A. Febbraio ◽  
Martin Whitham
Keyword(s):  
Skeletal Muscle ◽  
Meta Analysis ◽  
Data Sets ◽  
Biological Processes ◽  
Muscle Adaptation ◽  
Online Tool ◽  
Fully Integrated ◽  
Research Questions ◽  
Integrated Or ◽  
Adaptation To Exercise

Exercise stimulates a wide array of biological processes, but the mechanisms involved are incompletely understood. Many previous studies have adopted transcriptomic analyses of skeletal muscle to address particular research questions, a process that ultimately results in the collection of large amounts of publicly available data that has not been fully integrated or interrogated. To maximize the use of these available transcriptomic exercise data sets, we have downloaded and reanalyzed them and formulated the data into a searchable online tool, geneXX. GeneXX is highly intuitive and free and provides immediate information regarding the response of a transcript of interest to exercise in skeletal muscle. To demonstrate its utility, we carried out a meta-analysis on the included data sets and show transcript changes in skeletal muscle that persist regardless of sex, exercise mode, and duration, some of which have had minimal attention in the context of exercise. We also demonstrate how geneXX can be used to formulate novel hypotheses on the complex effects of exercise, using preliminary data already generated. This resource represents a valuable tool for researchers with interests in human skeletal muscle adaptation to exercise.

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