scholarly journals 1,25(OH) 2 vitamin D 3 stimulates active phosphate transport but not paracellular phosphate absorption in mouse intestine

2020 ◽  
Author(s):  
Nati Hernando ◽  
Eva Maria Pastor‐Arroyo ◽  
Joanne Marks ◽  
Udo Schnitzbauer ◽  
Thomas Knöpfel ◽  
...  
Keyword(s):  
Vitamin D ◽  
Phosphate Transport ◽  
Phosphate Absorption ◽  
Mouse Intestine ◽  
Vitamin D 3

Vitamin D Provision in High-Yield Dairy Cows During the Winter Housing Period

2014 ◽  
Vol 1 (1) ◽  
pp. 42-46
Author(s):  
L. Yuskiv ◽  
V. Vlizlo
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase Level ◽  
Mineral Metabolism ◽  
Physiological State ◽  
Blood Parameters ◽  
Postnatal Period ◽  
High Yield ◽  
Oral Supplementation ◽  
Calcium Phosphorus ◽  
Vitamin D 3

Aim. To investigate the vitamin D status in highly productive cows during winter housing period and effect of cholecalciferol by various ways of vitamin D 3 injection to cows in last days of gestation and after calving. Methods. Enzyme-linked immunoassay, spectrophotometry. Results. It has been stated that intramuscular injection of cholecalciferol into cows caused increase of the vitamin D 3 active metabolite – 25-OHD 3 , calcium, phosphorus and magnesium levels together with decrease of alkaline phosphatase level in pre- and post-natal periods. Oral supplementation makes little infl uence on the studied blood parameters of cows. Conclusions. Extrabuccal administration and oral supplementation of cholecalciferol in winter housing period to high-yield cows in the last days of gestation and after calving is accompanied by increased levels of its metabolites and their effect on mineral metabolism in the postnatal period. The nature of these changes depends on the mode of vitamin D administration and the physiological state of the cows.

Stanniocalcin: a novel protein regulating calcium and phosphate transport across mammalian intestine

1998 ◽  
Vol 274 (1) ◽  
pp. G96-G102 ◽  
Author(s):  
Karen L. Madsen ◽  
Michele M. Tavernini ◽  
Christine Yachimec ◽  
Donna L. Mendrick ◽  
Pedro J. Alfonso ◽  
...  
Keyword(s):  
Calcium Absorption ◽  
Short Circuit ◽  
Regulatory Protein ◽  
Chinese Hamster ◽  
Phosphate Transport ◽  
Simultaneous Increase ◽  
Rat Intestine ◽  
Glycoprotein Hormone ◽  
Phosphate Absorption ◽  
Ussing Chambers

Stanniocalcin (STC) is an anti-hypercalcemic glycoprotein hormone previously identified in the corpuscles of Stannius in bony fish and recently in the human genome. This study undertook to express human STC in Chinese hamster ovary (CHO) cells and to determine its effects on calcium and phosphate absorption in swine and rat intestine. Unidirectional mucosal-to-serosal ( J m→s) and serosal-to-mucosal ( J s→m)45Ca and32P fluxes were measured in vitro in duodenal tissue in voltage-clamped Ussing chambers. Addition of STC (10–100 ng/ml) to the serosal surface of the duodenum resulted in a simultaneous increase in calcium J m→s and J s→mfluxes, with a subsequent reduction in net calcium absorption. This was coupled with an STC-stimulated increase in phosphate absorption. Intestinal conductance was increased at the highest dose of STC (100 ng/ml) in swine tissue. The addition of STC to the mucosal surface had no effect on calcium and phosphate fluxes. STC at doses of 10–1,000 ng/ml had no effect on short-circuit current in any region of the rat intestine. In conclusion, human recombinant STC decreases the absorption of calcium and stimulates the absorption of phosphate in both swine and rat duodenum. STC is a novel regulatory protein that regulates mammalian intestinal calcium and phosphate transport.

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