scholarly journals Eliminating Nox2 reactive oxygen species production protects dystrophic skeletal muscle from pathological calcium influx assessedin vivoby manganese-enhanced magnetic resonance imaging

2016 ◽  
Vol 594 (21) ◽  
pp. 6395-6405 ◽  
Author(s):  
James A. Loehr ◽  
Gary R. Stinnett ◽  
Mayra Hernández-Rivera ◽  
Wesley T. Roten ◽  
Lon J. Wilson ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Reactive Oxygen Species ◽  
Skeletal Muscle ◽  
Magnetic Resonance ◽  
Reactive Oxygen Species Production ◽  
Calcium Influx ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Resonance Imaging ◽  
Species Production

scholarly journals Ultrasensitive magnetic resonance imaging of systemic reactive oxygen species in vivo for early diagnosis of sepsis using activatable nanoprobes

2019 ◽  
Vol 10 (13) ◽  
pp. 3770-3778 ◽  
Author(s):  
Huan Wang ◽  
Dongqin Yu ◽  
Bo Li ◽  
Zhen Liu ◽  
Jinsong Ren ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Reactive Oxygen Species ◽  
Magnetic Resonance ◽  
Early Diagnosis ◽  
Contrast Agents ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Resonance Imaging

Novel ROS-activated contrast agents are designed for magnetic resonance imaging of ROS for early diagnosis of sepsis.

scholarly journals Reactive Oxygen Species Generation in Human Cells by a Novel Magnetic Resonance Imaging Contrast Agent

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Li Wang ◽  
Eric Lin ◽  
Mary J. Johansen ◽  
Timothy Madden ◽  
Edward Felix ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Reactive Oxygen Species ◽  
Aqueous Solution ◽  
Magnetic Resonance ◽  
Cultured Cells ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Ros Production ◽  
Oxygen Species ◽  
Resonance Imaging

The novel positive-contrast magnetic resonance imaging (MRI) marker C4 consists of an aqueous solution of cobalt chloride (CoCl2) complexed with the chelator N-acetylcysteine (NAC). We evaluated whether the presence of C4 or its components would produce reactive oxygen species (ROS, including hydroxyl, peroxyl, or other reactive oxygen species) in cultured cells. Human cancer or normal cells were incubated with 1% (w/v) CoCl2·6H2O or 2% NAC or a combination of both (1% CoCl2·6H2O : 2% NAC in an aqueous solution, abbreviated as Co : NAC) in the presence or absence of H2O2. Intracellular ROS levels were measured and quantified by change in relative fluorescence units. Student’s t-tests were used. In all cell lines exposed to 1000 μM H2O2, the Co : NAC led to ≥94.7% suppression of ROS at 5 minutes and completely suppressed ROS at 60 and 90 minutes; NAC suppressed ROS by ≥76.6% at 5 minutes and by ≥94.5% at 90 minutes; and CoCl2·6H2O suppressed ROS by ≥37.2% at 30 minutes and by ≥48.6% at 90 minutes. These results demonstrate that neither Co : NAC nor its components generated ROS; rather, they suppressed ROS production in cultured cells, suggesting that C4 would not enhance ROS production in clinical use.

Sign in / Sign up

Export Citation Format

Share Document