scholarly journals Endogenous and exogenous control of gastrointestinal epithelial function: building on the legacy of Bayliss and Starling

2016 ◽  
Vol 595 (2) ◽  
pp. 423-432 ◽  
Author(s):  
Kim E. Barrett
Keyword(s):  
Exogenous Control

Somatic gene transfer in European sea bass (Dicentrarchus labrax): Optimal fish conditions and exogenous control of foreign genes

Aquaculture ◽  
2013 ◽  
Vol 388-391 ◽  
pp. 60-69 ◽  
Author(s):  
Iciar Muñoz ◽  
Silvia Zanuy ◽  
María José Mazón ◽  
Manuel Carrillo ◽  
Ana Gómez
Keyword(s):  
Gene Transfer ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
European Sea Bass ◽  
Somatic Gene ◽  
Foreign Genes ◽  
Somatic Gene Transfer ◽  
Exogenous Control

scholarly journals Exogenous control over intracellular acidification: Enhancement via proton caged compounds coupled to gold nanoparticles and an alternative pathway with DMSO

Data in Brief ◽  
2016 ◽  
Vol 6 ◽  
pp. 745-749 ◽  
Author(s):  
Marilena Carbone ◽  
Gianfranco Sabbatella ◽  
Simonetta Antonaroli ◽  
Hynd Remita ◽  
Viviana Orlando ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Alternative Pathway ◽  
Intracellular Acidification ◽  
Caged Compounds ◽  
Exogenous Control

Engineering Gene Control Circuits with Allosteric Ribozymes in Human Cells as a Medicine of the Future

2013 ◽  
pp. 860-883
Author(s):  
Robert Penchovsky
Keyword(s):  
Gene Expression ◽  
Cell Fate ◽  
Regulatory Networks ◽  
Control Of Gene Expression ◽  
Practical Applications ◽  
Gene Therapies ◽  
Sequencing Technologies ◽  
The Future ◽  
Control Circuits ◽  
Exogenous Control

Systems and synthetic biology promise to develop new approaches for analysis and design of complex gene expression regulatory networks in living cells with many practical applications to the pharmaceutical and biotech industries. In this chapter the development of novel universal strategies for exogenous control of gene expression is discussed. They are based on designer allosteric ribozymes that can function in the cell. The synthetic riboswitches are obtained by a patented computational procedure that provides fast and accurate modular designs with various Boolean logic functions. The riboswitches can be designed to sense in the cell either the presence or the absence of disease indicative RNA(s) or small molecules, and to switch on or off the gene expression of any exogenous protein. In addition, the riboswitches can be engineered to induce RNA interference or microRNA pathways that can conditionally down regulate the expression of key proteins in the cell. That can prevent a disease’s development. Therefore, the presented synthetic riboswitches can be used as truly universal cellular biosensors. Nowadays, disease indicative RNA(s) can be precisely identified by employing next-generation sequencing technologies with high accuracy . The methods can be employed not only for exogenous control of gene expression but also for re-programming the cell fate, anticancer, and antiviral gene therapies. Such approaches may be employed as potent molecular medicines of the future.

scholarly journals Exogenous control over intracellular acidification: Enhancement via proton caged compounds coupled to gold nanoparticles

2015 ◽  
Vol 1850 (11) ◽  
pp. 2304-2307 ◽  
Author(s):  
Marilena Carbone ◽  
Gianfranco Sabbatella ◽  
Simonetta Antonaroli ◽  
Hynd Remita ◽  
Viviana Orlando ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Intracellular Acidification ◽  
Caged Compounds ◽  
Exogenous Control

Deficits In The Exogenous Control Of Covert Visuospatial Attention In Children With Poor Motor Coordination

2010 ◽  
Vol 42 ◽  
pp. 429
Author(s):  
Yu-Ting Tseng ◽  
Chia-Liang Tsai ◽  
Chun-Hao Wang ◽  
Chien-Yu Pan ◽  
Kai-Wen Hsieh
Keyword(s):  
Motor Coordination ◽  
Visuospatial Attention ◽  
Exogenous Control

279 CYTOPLASMIC POLYADENYLATION-REGULATED GENE EXPRESSION DURING BOVINE OOCYTE MATURATION

2015 ◽  
Vol 27 (1) ◽  
pp. 228
Author(s):  
J. M. Reyes ◽  
P. J. Ross
Keyword(s):  
Oocyte Maturation ◽  
Tail Length ◽  
Transcript Abundance ◽  
Bovine Oocyte ◽  
Rna Seq ◽  
Cytoplasmic Polyadenylation ◽  
Transcript Levels ◽  
Regulated Gene Expression ◽  
Exogenous Control

Using RNA-seq of GV and MII oocytes we have described changes in polyadenylated transcript abundance that occur during in vitro bovine oocyte maturation (Reyes J. M., J. L. Chitwood, and P. J. Ross. 2013. Deciphering regulation of transcript abundance in maturing bovine oocytes. Poster session presented at International Plant & Animal Genome XXI. San Diego, CA). These changes can be attributed to transcript degradation, transcription, or transcript polyadenylation levels. The objectives of the present study were to determine the extent of cytoplasmic polyadenylation (CP) by measuring total and polyadenylated transcript abundance and poly(A) tail length in GV and MII oocytes for 8 (CCNB1, CPEB4, DNMT3B, FBXO43, EZH2, GDF9, PRDX2, and PAIP2) genes selected based on RNA-seq results. Oocytes were obtained by aspiration of abattoir-derived ovaries (GV) and in vitro maturation for 24 h (MII). Four pools of 40 oocytes were collected per stage. Enhanced green fluorescent protein (EGFP) cRNA was spiked into each sample before RNA extraction using the PicoPure RNA Isolation Kit. Extracted RNA was equally divided for cDNA synthesis using either random hexamers or anchored oligo(dT) primers to detect total and polyadenylated transcripts, respectively. Quantitative PCR (qPCR) of target genes, EGFP (exogenous control), and PPIA (endogenous control) was performed in duplicate for each replicate and gene. Relative transcript abundance was calculated using the 2[–ΔΔC(T)] method and statistically analysed using the Student's t-test. Transcript poly(A) tail length was determined for all but 2 genes (DNMT3B and EZH2) at the GV and MII stages using rapid amplification of cDNA ends poly(A) test (RACE-PAT). Two replicates of GV (n = 100) and MII (n = 100) pairs were collected to perform RACE-PAT followed by fragment analysis on a Bioanalyzer DNA 1000 chip. Polyadenylated RNA abundance levels matched those of the RNA-seq study for 7/8 genes using both PPIA and EGFP to normalise qPCR data, demonstrating the validity of RNA-seq results. Furthermore, total transcript levels for 6/8 and 7/8 genes remained unchanged when normalized to an endogenous and exogenous control, respectively. Combined, the results suggest a significant role for CP considering changes in polyadenylated transcript levels occur without changes in total RNA abundance. Also, changes in transcript abundance corresponded to differences in poly(A) tail length at the specific stages as determined by RACE-PAT. In conclusion, CP is the predominant mechanism responsible for changes in transcript abundance during oocyte maturation at least for the majority of the examined genes, though more in-depth studies are required to determine the global extent of CP. This study improved the atlas of CP-regulated genes potentially providing researchers with critical knowledge to improve in silico tools that predict genes regulated by CP based on presence, position, and distribution of motifs within the 3′ untranslated region.

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