CLDN4 silencing promotes proliferation and reduces chemotherapy sensitivity of gastric cancer cells through activation of the PI3K/Akt signalling pathway

2020 ◽  
Vol 105 (6) ◽  
pp. 979-988 ◽  
Author(s):  
Jie Luo ◽  
Huaiming Wang ◽  
Huanjie Chen ◽  
Guolian Gan ◽  
Yifeng Zheng
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Signalling Pathway ◽  
Gastric Cancer Cells ◽  
Chemotherapy Sensitivity

scholarly journals Golgi phosphoprotein-3 promotes invasiveness of gastric cancer cells through the mTOR signalling pathway

2019 ◽  
Vol 42 (2) ◽  
pp. E38-47 ◽  
Author(s):  
Jun Liu ◽  
Hongquan Wei ◽  
Liqin Lai ◽  
Yuanyuan Wang ◽  
Xiaoyu Han ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cell Invasion ◽  
Signalling Pathway ◽  
Gastric Cancer Cells ◽  
Poorly Differentiated ◽  
The Impact ◽  
Mtor Signalling ◽  
Mtor Signalling Pathway

Purpose: Golgi phosphoprotein-3 (GOLPH3) is an oncogene that is overexpressed in multiple cancers and is associated with poor prognosis. The aim of this study was to examine the impact of GOLPH3 on the migration and metastasis of gastric cancer cells. Methods: Following the shRNA-mediated knockdown of GOLPH3, we analyzed cytoskeletal reorganization and cell invasion, migration and adhesion, and determined the impact of components of the mammalian target of the rapamycin (mTOR) signalling pathway. Results: The GOLPH3 mRNA and protein expression were significantly lower in both SGC-7901 and MKN-28 cells as compared with poorly-differentiated BGC-823 cells. The GOLPH3 knockdown also significantly reduced cell invasion in all three cell lines through reduced migration as compared with the non-targeting control sequence group. The GOLPH3 knockdown also reduced F-actin in all three cell lines, and decreased cell adhesion in BGC-823 and SGC-7901 cells. Finally, p-mTOR, p70S6K, p-4EBP1 and RhoA protein levels were significantly downregulated in shGOLPH3-1-treated cells. Conclusions: In conclusion, GOLPH3 increased in poorly-differentiated gastric cancer cells, activating the mTOR-70S6K/4EBP1-RhoA signalling pathway to promote the migration and metastasis of gastric cancer cells.

scholarly journals Activation of ERK signaling pathway reduces the chemotherapy sensitivity in gastric cancer cells

2008 ◽  
Vol 16 (20) ◽  
pp. 2211
Author(s):  
Shi-Quan Liu ◽  
Hong-Gang Yu ◽  
Guo-Du Tang ◽  
Jie-An Huang ◽  
Jie-Ping Yu
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Erk Signaling ◽  
Gastric Cancer Cells ◽  
Chemotherapy Sensitivity
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