scholarly journals Gene expression variability in human skeletal muscle transcriptome responses to acute resistance exercise

2019 ◽  
Vol 104 (5) ◽  
pp. 625-629 ◽  
Author(s):  
Jacob T. Bonafiglia ◽  
Keir J. Menzies ◽  
Brendon J. Gurd
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Resistance Exercise ◽  
Human Skeletal Muscle ◽  
Expression Variability ◽  
Skeletal Muscle Transcriptome

scholarly journals Relationship Between METTL21C Gene Expression and Exercise in Human Skeletal Muscle: A Meta-Analysis

2021 ◽  
Vol 30 (1) ◽  
pp. 102-109
Author(s):  
Kyung-Wan Baek ◽  
Ji-Seok Kim ◽  
Jun-Il Yoo
Keyword(s):  
Gene Expression ◽  
Physical Activity ◽  
Skeletal Muscle ◽  
Exercise Training ◽  
Resistance Exercise ◽  
Human Skeletal Muscle ◽  
Meta Analysis ◽  
Exercise Group ◽  
Aerobic Exercise Training ◽  
Control Group

PURPOSE: Recently, METTL21C has been identified as a potential pleiotropic gene for osteoporosis and sarcopenia. The purpose of this study was to collect gene expression datasets of human skeletal muscle transcriptome and to determine their relationship to exercise through meta-analysis.METHODS: MetaMEx was used to determine whether METTL21C in human skeletal muscle was associated with age, sex, physical activity and obesity. In addition, the difference in gene expression of METTL21C according to exercise duration and exercise type was confirmed. Using MetaMEx, top 300 genes (positive and negative, respectively) with a high correlation with METTL21C were selected, and gene ontology analysis was performed to identify related pathways.RESULTS:The expression of METTL21C gene in human skeletal muscle was significantly lower in the elderly than in young subjects (p<.0001), and significantly lower in female than in male (p<.0001). Also, the obese subjects were significantly lower than lean subjects (p<.0001). However, subjects with high level of physical activity had significantly higher expression of METTL21C than subjects with low levels of physical activity (p<.0001). Acute resistance exercise (p<.0001) and acute high-intensity interval training (p<.05) were found to have significantly higher expression of METTL21C in the skeletal muscle of the exercise group compared to the control group. Aerobic exercise training (p<.0001) and resistance exercise training (p<.0001) showed significantly higher expression of METTL21C in the skeletal muscle of the exercise group compared to the control group.CONCLUSIONS: Physical activity and exercise is important to prevent and treat osteosarcopenia because it can increase the expression of METTL21C in human skeletal muscle and maintain bone and muscle homeostasis.

scholarly journals Metabolic adaptations in skeletal muscle after 84 days of bed rest with and without concurrent flywheel resistance exercise

2017 ◽  
Vol 122 (1) ◽  
pp. 96-103 ◽  
Author(s):  
José M. Irimia ◽  
Mario Guerrero ◽  
Paula Rodriguez-Miguelez ◽  
Joan A. Cadefau ◽  
Per A. Tesch ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Resistance Exercise ◽  
Human Skeletal Muscle ◽  
Citrate Synthase ◽  
Glycogen Synthase ◽  
Vastus Lateralis ◽  
Bed Rest ◽  
Metabolic Adaptations

As metabolic changes in human skeletal muscle after long-term (simulated) spaceflight are not well understood, this study examined the effects of long-term microgravity, with and without concurrent resistance exercise, on skeletal muscle oxidative and glycolytic capacity. Twenty-one men were subjected to 84 days head-down tilt bed rest with (BRE; n = 9) or without (BR; n = 12) concurrent flywheel resistance exercise. Activity and gene expression of glycogen synthase, glycogen phosphorylase (GPh), hexokinase, phosphofructokinase-1 (PFK-1), and citrate synthase (CS), as well as gene expression of succinate dehydrogenase (SDH), vascular endothelial growth factor (VEFG), peroxisome proliferator-activated receptor gamma coactivator-1 (PGC-1α), and myostatin, were analyzed in samples from m. vastus lateralis collected before and after bed rest. Activity and gene expression of enzymes controlling oxidative metabolism (CS, SDH) decreased in BR but were partially maintained in BRE. Activity of enzymes regulating anaerobic glycolysis (GPh, PFK-1) was unchanged in BR. Resistance exercise increased the activity of GPh. PGC-1α and VEGF expression decreased in both BR and BRE. Myostatin increased in BR but decreased in BRE after bed rest. The analyses of these unique samples indicate that long-term microgravity induces marked alterations in the oxidative, but not the glycolytic, energy system. The proposed flywheel resistance exercise was effective in counteracting some of the metabolic alterations triggered by 84-day bed rest. Given the disparity between gene expression vs. enzyme activity in several key metabolic markers, posttranscriptional mechanisms should be explored to fully evaluate metabolic adaptations to long-term microgravity with/without exercise countermeasures in human skeletal muscle.

scholarly journals Resistance Exercise Reverses Aging in Human Skeletal Muscle

PLoS ONE ◽  
2007 ◽  
Vol 2 (5) ◽  
pp. e465 ◽  
Author(s):  
Simon Melov ◽  
Mark A. Tarnopolsky ◽  
Kenneth Beckman ◽  
Krysta Felkey ◽  
Alan Hubbard
Keyword(s):  
Skeletal Muscle ◽  
Resistance Exercise ◽  
Human Skeletal Muscle

scholarly journals Promoter-specific regulation of PPARGC1A gene expression in human skeletal muscle

2015 ◽  
Vol 55 (2) ◽  
pp. 159-168 ◽  
Author(s):  
Daniil V Popov ◽  
Evgeny A Lysenko ◽  
Tatiana F Vepkhvadze ◽  
Nadia S Kurochkina ◽  
Pavel A Maknovskii ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Stop Codon ◽  
Constitutive Expression ◽  
Alternative Promoter ◽  
Specific Expression ◽  
Post Exercise ◽  
Specific Regulation ◽  
Intensity Of Exercise

The goal of this study was to identify unknown transcription start sites of thePPARGC1A(PGC-1α) gene in human skeletal muscle and investigate the promoter-specific regulation ofPGC-1αgene expression in human skeletal muscle. Ten amateur endurance-trained athletes performed high- and low-intensity exercise sessions (70 min, 70% or 50%o2max). High-throughput RNA sequencing and exon–exon junction mapping were applied to analyse muscle samples obtained at rest and after exercise.PGC-1αpromoter-specific expression and activation of regulators of PGC-1α gene expression (AMPK, p38 MAPK, CaMKII, PKA and CREB1) after exercise were evaluated using qPCR and western blot. Our study has demonstrated that during post-exercise recovery, human skeletal muscle expresses thePGC-1αgene via two promoters only. As previously described, the additional exon 7a that contains a stop codon was found in all samples. Importantly, only minor levels of other splice site variants were found (and not in all samples). Constitutive expressionPGC-1αgene occurs via the canonical promoter, independent of exercise intensity and exercise-induced increase of AMPKThr172phosphorylation level. Expression ofPGC-1αgene via the alternative promoter is increased of two orders after exercise. This post-exercise expression is highly dependent on the intensity of exercise. There is an apparent association between expression via the alternative promoter and activation of CREB1.

scholarly journals Heat stress enhances mTOR signaling after resistance exercise in human skeletal muscle

2011 ◽  
Vol 61 (2) ◽  
pp. 131-140 ◽  
Author(s):  
Ryo Kakigi ◽  
Hisashi Naito ◽  
Yuji Ogura ◽  
Hiroyuki Kobayashi ◽  
Norio Saga ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Heat Stress ◽  
Resistance Exercise ◽  
Human Skeletal Muscle ◽  
Mtor Signaling
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