scholarly journals TRPV1channels in human skeletal muscle feed arteries: implications for vascular function

2017 ◽  
Vol 102 (9) ◽  
pp. 1245-1258 ◽  
Author(s):  
Stephen J. Ives ◽  
Song Young Park ◽  
Oh Sung Kwon ◽  
Jayson R. Gifford ◽  
Robert H. I. Andtbacka ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Vascular Function ◽  
Human Skeletal Muscle ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries

Aging induces muscle-specific impairment of endothelium-dependent dilation in skeletal muscle feed arteries

2002 ◽  
Vol 93 (5) ◽  
pp. 1685-1690 ◽  
Author(s):  
Christopher R. Woodman ◽  
Elmer M. Price ◽  
M. Harold Laughlin
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Vascular Function ◽  
Fischer 344 ◽  
Old Rats ◽  
The Right ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries

We tested the hypothesis that aging decreases endothelium-dependent vasodilation in feed arteries perfusing rat skeletal muscle. In addition, we tested the hypothesis that attenuated vasodilator responses are associated with decreased endothelial nitric oxide synthase (eNOS) and superoxide dismutase-1 (SOD-1) expression. Soleus feed arteries (SFA) and gastrocnemius feed arteries (GFA) were isolated from young (4 mo) and old (24 mo) male Fischer 344 rats. Feed arteries from the right hindlimb were cannulated with two glass micropipettes for examination of endothelium-dependent [acetylcholine (ACh)] and endothelium-independent [adenosine (Ado) or sodium nitroprusside (SNP)] vasodilator function. Feed arteries from the left hindlimb were frozen and used to assess eNOS and SOD-1 protein and mRNA expression. In SFA, endothelium-dependent dilation to ACh was reduced in old rats (0.9 ± 0.04 vs. 0.8 ± 0.03), whereas dilator responses to Ado and SNP were similar in SFA of young and old rats. In GFA, vasodilator responses to ACh, Ado, and SNP were not altered by age. eNOS and SOD-1 protein expression declined with age in SFA (−71 and −54%, respectively) but not in GFA. eNOS and SOD-1 mRNA expression were not altered by age in SFA or GFA. Collectively, these data indicate aging induces muscle-specific impairment of endothelium-dependent vascular function in SFA.

scholarly journals Vasodilatory function in human skeletal muscle feed arteries with advancing age: the role of adropin

2019 ◽  
Vol 597 (7) ◽  
pp. 1791-1804 ◽  
Author(s):  
Oh Sung Kwon ◽  
Robert H. I. Andtbacka ◽  
John R. Hyngstrom ◽  
Russell S. Richardson
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Vasodilatory Function ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries

The Role of TRPV Ion Channels in Heat-induced Sympatholysis of Human Skeletal Muscle Feed Arteries

2014 ◽  
Vol 46 ◽  
pp. 328-329
Author(s):  
Jayson R. Gifford ◽  
Stephen J. Ives ◽  
Song Y. Park ◽  
Robert H.I. Andtbacka ◽  
Joel D. Trinity ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Ion Channels ◽  
Human Skeletal Muscle ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries

scholarly journals Heat and α1-adrenergic responsiveness in human skeletal muscle feed arteries: the role of nitric oxide

2012 ◽  
Vol 113 (11) ◽  
pp. 1690-1698 ◽  
Author(s):  
Stephen J. Ives ◽  
Robert H. I. Andtbacka ◽  
Sun Hyung Kwon ◽  
Yan-Ting Shiu ◽  
Ting Ruan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Adrenergic Receptors ◽  
Adrenergic Receptor ◽  
Human Skeletal Muscle ◽  
Bovine Endothelial Cells ◽  
No Bioavailability ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries

Increased local temperature exerts a sympatholytic effect on human skeletal muscle feed arteries. We hypothesized that this attenuated α1-adrenergic receptor responsiveness may be due to a temperature-induced increase in nitric oxide (NO) bioavailability, thereby reducing the impact of the α1-adrenergic receptor agonist phenylephrine (PE). Thirteen human skeletal muscle feed arteries were harvested, and wire myography was used to generate PE concentration-response curves at 37°C and 39°C, with and without the NO synthase (NOS) inhibitor NG-monomethyl-l-arginine (l-NMMA). A subset of arteries ( n = 4) were exposed to 37°C or 39°C, and the protein content of endothelial NOS (eNOS) and α1-adrenergic receptors was determined by Western blot analysis. Additionally, cultured bovine endothelial cells were exposed to static or shear stress conditions at 37°C and 39°C and assayed for eNOS activation (phosphorylation at Ser1177), eNOS expression, and NO metabolites [nitrate + nitrite (NOx)]. Maximal PE-induced vasocontraction (PEmax) was lower at 39°C than at 37°C [39 ± 10 vs. 84 ± 30% maximal response to 100 mM KCl (KClmax)]. NO blockade restored vasocontraction at 39°C to that achieved at 37°C (80 ± 26% KClmax). Western blot analysis of the feed arteries revealed that heating increased eNOS protein, but not α1-adrenergic receptors. Heating of bovine endothelial cells resulted in greater shear stress-induced eNOS activation and NOx production. Together, these data reveal for the first time that, in human skeletal muscle feed arteries, NO blockade can restore the heat-attenuated α1-adrenergic receptor-mediated vasocontraction and implicate endothelium-derived NO bioavailability as a major contributor to heat-induced sympatholysis. Consequently, these findings highlight the important role of vasodilators in modulating the vascular response to vasoconstrictors.

scholarly journals α1- and α2-Adrenergic responsiveness in human skeletal muscle feed arteries: the role of TRPV ion channels in heat-induced sympatholysis

2014 ◽  
Vol 307 (9) ◽  
pp. H1288-H1297 ◽  
Author(s):  
Jayson R. Gifford ◽  
Stephen J. Ives ◽  
Song-Young Park ◽  
Robert H. I. Andtbacka ◽  
John R. Hyngstrom ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Ion Channels ◽  
Human Skeletal Muscle ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Ruthenium Red ◽  
Temperature Sensitive ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries

The purpose of this study was to determine if heat inhibits α2-adrenergic vasocontraction, similarly to α1-adrenergic contraction, in isolated human skeletal muscle feed arteries (SMFA) and elucidate the role of the temperature-sensitive vanilloid-type transient receptor potential (TRPV) ion channels in this response. Isolated SMFA from 37 subjects were studied using wire myography. α1 [Phenylephrine (PE)]- and α2 [dexmedetomidine (DEX)]-contractions were induced at 37 and 39°C with and without TRPV family and TRPV4-specific inhibition [ruthenium red (RR) and RN-1734, respectively]. Endothelial function [acetylcholine (ACh)] and smooth muscle function [sodium nitroprusside (SNP) and potassium chloride (KCl)] were also assessed under these conditions. Heat and TRPV inhibition was further examined in endothelium-denuded arteries. Contraction data are reported as a percentage of maximal contraction elicited by 100 mM KCl (LTmax). DEX elicited a small and variable contractile response, one-fifth the magnitude of PE, which was not as clearly attenuated when heated from 37 to 39°C (12 ± 4 to 6 ± 2% LTmax; P = 0.18) as were PE-induced contractions (59 ± 5 to 24 ± 4% LTmax; P < 0.05). Both forms of TRPV inhibition restored PE-induced contraction at 39°C (P < 0.05) implicating these channels, particularly the TRPV4 channels, in the heat-induced attenuation of α1-adrenergic vasocontraction. TRPV inhibition significantly blunted ACh relaxation while denudation prevented heat-induced sympatholysis without having an additive effect when combined with TRPV inhibition. In conclusion, physiological increases in temperature elicit a sympatholysis-like inhibition of α1-adrenergic vasocontraction in human SMFA that appears to be mediated by endothelial TRPV4 ion channels.

scholarly journals Evidence of the regulatory potential of human skeletal muscle feed arteries

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Stephen James Ives ◽  
Robert Hans Ingemar Andtbacka ◽  
R. Dirk Noyes ◽  
Anthony Donato ◽  
Song Young Park ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Regulatory Potential ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries

scholarly journals α1-Adrenergic responsiveness in human skeletal muscle feed arteries: the impact of reducing extracellular pH

2012 ◽  
Vol 98 (1) ◽  
pp. 256-267 ◽  
Author(s):  
Stephen J. Ives ◽  
Robert H. I. Andtbacka ◽  
R. Dirk Noyes ◽  
R. Garrett Morgan ◽  
Jayson R. Gifford ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Extracellular Ph ◽  
The Impact ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries

scholarly journals Human skeletal muscle feed arteries: evidence of regulatory potential

2012 ◽  
Vol 206 (2) ◽  
pp. 135-141 ◽  
Author(s):  
S. J. Ives ◽  
R. H. I. Andtbacka ◽  
S.-Y. Park ◽  
A. J. Donato ◽  
J. R. Gifford ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Regulatory Potential ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries
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