ISOLATION AND IDENTIFICATION OF RHIZOSPHERIC BACTERIA ASSOCIATED WITH LEMONGRASS FOR POTENTIAL BIOREMEDIATION

2015 ◽  
Vol 77 (24) ◽  
Author(s):  
Najwa Husna Sanusi ◽  
Phang Ing Chia ◽  
Noor Faizul Hadry Nordin
Keyword(s):  
Future Research ◽  
Rrna Gene ◽  
Public Attention ◽  
Isolation And Identification ◽  
Severe Problem ◽  
Rhizospheric Bacteria ◽  
Large Numbers ◽  
Degrading Bacteria ◽  
Cleaner Technology ◽  
Remediation Process

Contamination of soil and groundwater pollution is a severe problem, has been attracting considerable public attention over the last decades. With the demand for green and cleaner technology for remediation process, there is an increased interest in moving away from conventional technologies towards bioremediation technologies. Rhizospheric zone is a suitable place for harboring bacteria that are capable to utilize chemical compounds which will be used either to facilitate growth of bacteria or the host plants. Identification of the specific microbial members should allow for better strategies to enhance biodegradation. This study aimed to isolate and identify the rhizospheric associated microbes of lemongrass (Cymbopogon citratus), a plant that commonly available in South East Asia, which could be used in future research on degradation studies of dibenzofuran. This probably is due to their ability to harbor large numbers of bacteria on their highly branched root systems. A total of 68 strains of dibenzofuran (DF)- degrading bacteria isolated from the rhizospheric soil of lemon grass from 2 different unpolluted sites were characterized. The isolates showed the ability to utilize dibenzofuran as the sole carbon and energy source up to 40 ppm. Identification of the isolates based on 16S rRNA gene sequence assigned them as members of the phyla Proteobacteria and Firmicutes, among which those of the genera, Proteobacteria were most abundant. The presented results indicated the potential of these bacterial isolates in bioremediation of dibenzofuran-contaminated soil.

scholarly journals Isolation and Identification of Sodium Fluoroacetate Degrading Bacteria from Caprine Rumen in Brazil

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Expedito K. A. Camboim ◽  
Arthur P. Almeida ◽  
Michelle Z. Tadra-Sfeir ◽  
Felício G. Junior ◽  
Paulo P. Andrade ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
16S Rrna Gene Sequencing ◽  
Positive Control ◽  
Control Measures ◽  
Rrna Gene ◽  
Isolation And Identification ◽  
Poisonous Plants ◽  
Degrading Bacteria ◽  
Rrna Gene Sequencing ◽  
Orbital Shaker

The objective of this paper was to report the isolation of two fluoroacetate degrading bacteria from the rumen of goats. The animals were adult goats, males, crossbred, with rumen fistula, fed with hay, and native pasture. The rumen fluid was obtained through the rumen fistula and immediately was inoculated 100 μL in mineral medium added with 20 mmol L−1sodium fluoroacetate (SF), incubated at 39°C in an orbital shaker.Pseudomonas fluorescens(strain DSM 8341) was used as positive control for fluoroacetate dehalogenase activity. Two isolates were identified by 16S rRNA gene sequencing asPigmentiphaga kullae(ECPB08) andAncylobacter dichloromethanicus(ECPB09). These bacteria degraded sodium fluoroacetate, releasing 20 mmol L−1of fluoride ion after 32 hours of incubation in Brunner medium containing 20 mmol L−1of SF. There are no previous reports of fluoroacetate dehalogenase activity forP. kullaeandA. dichloromethanicus. Control measures to prevent plant intoxication, including use of fences, herbicides, or other methods of eliminating poisonous plants, have been unsuccessful to avoid poisoning by fluoroacetate containing plants in Brazil. In this way,P. kullaeandA. dichloromethanicusmay be used to colonize the rumen of susceptible animals to avoid intoxication by fluoroacetate containing plants.

ISOLATION AND IDENTIFICATION OF 3-CHLOROPROPIONIC ACID DEGRADING BACTERIUM FROM MARINE SPONGE

2015 ◽  
Vol 77 (25) ◽  
Author(s):  
Nurul Hanani Mohd Sufian ◽  
Mohd Azrul Naim ◽  
Tengku Haziyamin Tengku Abdul Hamid ◽  
Fahrul Huyop ◽  
Azzmer Azzar Abdul Hamid
Keyword(s):  
Marine Sponge ◽  
Chloride Ion ◽  
Rrna Gene ◽  
Active Components ◽  
Ion Release ◽  
Isolation And Identification ◽  
16S Rrna Gene Analysis ◽  
Bacteria Growth ◽  
Degrading Bacterium ◽  
Degrading Bacteria

Synthetic haloalkanoic acids often applied as active components in herbicides are toxic to the environment and harmful to the living organisms. These compounds are widely released to the environment due to their routine use by agricultural activities.  In recent years, accumulation of haloalkanoic acids was emerged in marine environment. In this study, a 3CP-degrading bacterium which designated as strain H4 was successfully isolated from marine sponge Gelliodes sp. that capable of degrading 3CP as the sole carbon and energy source. The bacteria growth on solid minimal media containing 3CP was the evident for the presence of dehalogenase enzyme. In liquid medium, the doubling time of the cells for strain H4 was 56.82 ± 0.1 h while the maximum chloride ion release was 2.03 ± 0.01 mM. The 16S rDNA sequence of strain H4 was obtained via 16s rRNA gene analysis (1000 bp) and it was closely related to Bacillus aryabhattai B8W22 (99% similarity). To the best of knowledge, this report is the first report detailing haloalkanoic acid degrading bacteria from marine sponge in coastal area of Malaysia.

scholarly journals Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces

2019 ◽  
Vol 20 (6) ◽  
Author(s):  
TOTO ISWANTO ◽  
MAYA SHOVITRI ◽  
ALI ALTWAY ◽  
TRI WIDJAJA ◽  
DINIHARI INDAH KUSUMAWATI ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Coffee Bean ◽  
Rrna Gene Sequence ◽  
Isolation And Identification ◽  
Coffee Pulp ◽  
Degrading Bacteria ◽  
Coffee Pulp Waste

Abstract. Iswanto T, Shovitri M, Altway A, Widjaja T, Kusumawati DI, Lisdiyanti P. 2019. Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces. Biodiversitas 20: 1580-1587. The present study deals with the isolation and identification of caffeine-degrading bacteria obtained from the caffeine contaminated environment or caffeinated wastes. These bacteria are useful for various biotechnological applications especially in increasing the potential utilization of caffeinated wastes and producing the high-value chemicals. The suspected caffeine-degrading bacteria have been isolated from the soil of coffee plantation area, coffee pulp waste, and the excreted coffee bean in fresh feces of Luwak (Paradoxurus hermaphroditus or Asian Palm Civet) by growing them on the caffeinated agar medium (CAM) containing basal salt medium (M9) and caffeine as a sole source of carbon and nitrogen. CAM-supplemented with 1.5 to 10 g L-1 of caffeine has been used for screening of the potential bacteria which able to grow in high caffeine concentration. Molecular identification based on 16S rRNA gene sequence was performed to identify the selected bacteria. The result revealed that there were 11 and 3 strains of 12 selected bacteria which could grow on the CAM-supplemented with caffeine up to 7 and 10 g L-1, respectively. Based on 16S rRNA gene sequence and phylogenetic analysis, those bacteria were from 5 Gram-negative species, namely Pseudomonas japonica (4/12), Methylobacterium populi (5/12), Raoultella ornithinolytica (1/12), Klebsiella quasipneumoniae (1/12), and Stenotrophomonas chelatiphaga (1/12). Further investigations to determine their metabolic pathway, enzyme, and growth kinetics in the caffeinated medium may provide insights into its possible utilization for scientific or other applications.

scholarly journals Influence of Human Activities on Broad-Scale Estuarine-Marine Habitats Using Omics-Based Approaches Applied to Marine Sediments

2019 ◽  
Vol 7 (10) ◽  
pp. 419 ◽  
Author(s):  
Shah ◽  
Crosswell ◽  
Metcalfe ◽  
Carlin ◽  
Morrison ◽  
...  
Keyword(s):  
Marine Sediments ◽  
Human Activities ◽  
Large Scale ◽  
Urban Expansion ◽  
Nitrite Reduction ◽  
Future Research ◽  
Rrna Gene ◽  
Marine Habitats ◽  
Degrading Bacteria ◽  
Insight Into

Rapid urban expansion and increased human activities have led to the progressive deterioration of many marine ecosystems. The diverse microbial communities that inhabit these ecosystems are believed to influence large-scale geochemical processes and, as such, analyzing their composition and functional metabolism can be a means to assessing an ecosystem’s resilience to physical and chemical perturbations, or at the very least provide baseline information and insight into future research needs. Here we show the utilization of organic and inorganic contaminant screening coupled with metabolomics and bacterial 16S rRNA gene sequencing to assess the microbial community structure of marine sediments and their functional metabolic output. The sediments collected from Moreton Bay (Queensland, Australia) contained low levels of organic and inorganic contaminants, typically below guideline levels. The sequencing dataset suggest that sulfur and nitrite reduction, dehalogenation, ammonia oxidation, and xylan degradation were the major metabolic functions. The community metabolites suggest a level of functional homogeneity down the 40-cm core depth sampled, with sediment habitat identified as a significant driver for metabolic differences. The communities present in river and sandy channel samples were found to be the most active, with the river habitats likely to be dominated by photoheterotrophs that utilized carbohydrates, fatty acids and alcohols as well as reduce nitrates to release atmospheric nitrogen and oxidize sulfur. Bioturbated mud habitats showed overlapping faunal activity between riverine and sandy ecosystems. Nitrogen-fixing bacteria and lignin-degrading bacteria were most abundant in the sandy channel and bioturbated mud, respectively. The use of omics-based approaches provide greater insight into the functional metabolism of these impacted habitats, extending beyond discrete monitoring to encompassing whole community profiling that represents true phenotypical outputs. Ongoing omics-based monitoring that focuses on more targeted pathway analyses is recommended in order to quantify the flux changes within these systems and establish variations from these baseline measurements.

scholarly journals Isolation and identification of fenobucarb degrading bacteria from Pangalengan farm land

2021 ◽  
Vol 883 (1) ◽  
pp. 012007
Author(s):  
A Akhdiya ◽  
R A Sanjaya ◽  
Wartono
Keyword(s):  
Soil Microbes ◽  
Agricultural Soils ◽  
Soil Samples ◽  
Haemolytic Activity ◽  
Rrna Gene ◽  
Isolation And Identification ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Degrading Bacteria

Abstract The long term excessive use of pesticides can lead to their residues accumulation in the soils. Soil microbes were considered to convert the residues into harmless compounds, however the indigenous soil microbes having those beneficial properties are limited. Therefore this study aimed to isolate, select and identify the fenobucarb insecticide-degrading bacteria from agricultural soils. The soil samples were collected from the vegetable fields in Pangalengan, West Java, Indonesia. Isolation of the bacteria was conducted using Nitrate Mineral Salt Agar suplemented by 100 ppm of a fenobucarb. The bacteria isolates were selected based on its hypersensitive response, haemolytic activity, and its ability to degrade fenobucarb. The selected isolates was identified base on sequences of 16S rRNA gene. Twenty nine bacteria were isolated from four soil samples and 23 of the isolates were not potentially phytopathogenic and non haemolytic. The best three isolates that could degrade 94.2%, 94.5% and 95.47% fenobucarb residue are B41, B54 and B83 isolates, respectively. The 16S rDNA Sequence analysis showed that B41 and B83 isolates have 100% similarity to Bacillus thuringiensis MYBT 18426B54, while B54 isolate has 99% similarity to Bacillus luciferensis LMG 18422. These isolates are potential to be developed as a bioremediation agent.

scholarly journals Isolation and Identification of Polystyrene Degrading Bacteria from Zophobas morio’s Gut

2021 ◽  
Vol 18 (8) ◽  
Author(s):  
Kian Meng TAN ◽  
Noor Akhmazillah MOHD FAUZI ◽  
Angzzas Sari MOHD KASSIM ◽  
Aliff Hisyam A RAZAK ◽  
Kamarul Rahim KAMARUDIN
Keyword(s):  
Bacillus Megaterium ◽  
Agar Plate ◽  
Morphological Characterization ◽  
Expanded Polystyrene ◽  
Rrna Gene ◽  
Triphenyltetrazolium Chloride ◽  
Isolation And Identification ◽  
Degrading Bacteria ◽  
Close Relationship ◽  
Promising Source

Polystyrene (PS) and expanded polystyrene (EPS) are commodity plastics widely used in many industries. The increase of PS in the environment causes ecological threats. It has been observed that the beetle larvae feed on plastic packages, and this has drawn the researcher’s attention towards the complex system within the larvae’s gut where relationships between fungi, bacteria, and the insect host have been established. The objective of this study is to isolate and identify PS-degrading microbe from super worms. The study used polystyrene as a sole carbon source to isolate the positive PS-degrading bacteria. Five bacterial colonies which showed positive growth on the modified PS-agar plate were isolated. All five colonies of the PS-degrading bacterial isolates were identified using partial 16 S rRNA gene sequencing. The results showed a close relationship between Bacillus aryabhattai and Bacillus megaterium strains. An examination via biochemical studies for distinction was implemented further. Based on the morphological characterization and taxonomic method, the isolates were identified as Bacillus megaterium. The isolates' viability was tested using 2,3,5-triphenyltetrazolium chloride (TTC), and positive results were obtained with red-colored insoluble triphenylformazan (TPF) formed within 5 to 7 days. The isolation and identification of these bacteria from Zophobas morio’s gut may provide a promising source of a plastic degrading enzyme that can be further studied and manipulated in developing enzymatic biodegradation in plastic waste management.

scholarly journals Effect of Yuzu (Citrus junos) Seed Limonoids and Spermine on Intestinal Microbiota and Hypothalamic Tissue in the Sandhoff Disease Mouse Model

2021 ◽  
Vol 9 (1) ◽  
pp. 17
Author(s):  
Mayumi Minamisawa ◽  
Takuma Suzumura ◽  
Sudeep Bose ◽  
Tetsuyuki Taniai ◽  
Gota Kawai ◽  
...  
Keyword(s):  
Mouse Model ◽  
Intestinal Microbiota ◽  
Neuronal Degeneration ◽  
Sandhoff Disease ◽  
Short Chain Fatty Acids ◽  
Rrna Gene ◽  
Wild Type ◽  
Degrading Bacteria ◽  
Citrus Junos ◽  
Hypothalamic Tissue

The effect of limonoids and spermine (Spm) extracted from yuzu (Citrus junos) seeds on the gut and the brain in a mouse model with Sandhoff disease (SD) was investigated. Wild-type and SD mice were fed a normal diet, or a diet supplemented with limonoid, Spm, or limonoid + Spm for 14–18 weeks, and then 16S rRNA gene amplicon sequencing with extracted DNA from their feces was executed. For SD control mice, intestinal microbiota was mostly composed of Lactobacillus and linked to dysbiosis. For SD and wild-type mice fed with limonoids + Spm or limonoids alone, intestinal microbiota was rich in mucin-degrading bacteria, including Bacteroidetes, Verrucomicrobia, and Firmicutes, and displayed a higher production of short-chain fatty acids and immunoglobulin A. Additionally, SD mice fed with limonoids + Spm or limonoids alone had less inflammation in hypothalamic tissues and displayed a greater number of neurons. Administration of limonoids and/or Spm improved the proportions of beneficial intestinal microbiota to host health and reduced neuronal degeneration in SD mice. Yuzu seed limonoids and Spermine may help to maintain the homeostasis of intestinal microbiota and hypothalamic tissue in the SD mouse model.

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