A NOBLE MOLECULAR IMPRINT POLYMER BIOSENSOR FOR CAFFEIC ACID DETECTION IN ORTHOSIPHON STAMINEUS EXTRACTS

2015 ◽  
Vol 77 (7) ◽  
Author(s):  
A.K.M. Shafiqul Islam ◽  
Hemavathi Krishnan ◽  
Harbant Singh ◽  
Mohd Noor Ahmad

A molecular imprint polymer (MIP) biosensor has been developed to determine caffeic acid in misai kucing (Orthosiphon stamineus) samples. The simulation of HyperChem 8.0 software gave a suitable template and functional monomer ratio for the MIP preparation. The MIPs were prepared by non-covalent bulk polymer approach. The analytical performance of MIP and NIP studies were based on the frequency change of mass sensitive quartz crystal microbalance sensor. The MIP biosensor showed good sensitivity to caffeic acid from 1.5 ng/ml - 12.5 ng/ml with a R2 value of 0.98 whereas NIP sensor showed very low response. The caffeic acid in O. stamineus extract and two commercial products were quantified using the MIP biosensor

2021 ◽  
Vol 57 (6) ◽  
pp. 671-679
Author(s):  
A. K. M. Shafiqul Islam ◽  
Hemavathi Krishnan ◽  
Mohd Noor Ahmad ◽  
Pubalan Nadaraja ◽  
A. B. M. Helal Uddin

2022 ◽  
Vol 18 (1) ◽  
pp. 171-181
Author(s):  
Alonso Sierra-Res ◽  
Berenice Robles-Her ◽  
María J. Bernad- ◽  
Roberto Día ◽  
Sheila I. Peñ ◽  
...  

2014 ◽  
Vol 530-531 ◽  
pp. 54-57 ◽  
Author(s):  
Masruroh ◽  
D.J.D.H. Djoko ◽  
Lalu A. Didik ◽  
Eka Rahmawati ◽  
Masdiana Pagaga ◽  
...  

This research is focused on the synthesis of a polystiren layer for biosensors based on a Quartz Crystal Microbalance sensor (QCM) to immobilize the biomolecule. The polystyrene thin film was deposited by means of spin coating method with various solvents, such as chloroform, toluene, xylene, and tetrahydrofuran (THF), containing a 3% polystyrene solution by mass. The morphologies of the polystyrene layers were observed via SEM/EDx. The polystyrene surface coated using chloroform as the solvent has a rougher morphology and the largest diameter pores compared with the other solvents. The result show the polystyrene surface coating produced with chloroform caused a higher frequency change, which resulted in the binding of a wider range of biomolecules.


2012 ◽  
Vol 12 (1) ◽  
pp. 563-567 ◽  
Author(s):  
Yasuhiro Kosaki ◽  
Kosuke Takano ◽  
Daniel Citterio ◽  
Koji Suzuki ◽  
Seimei Shiratori

RSC Advances ◽  
2021 ◽  
Vol 11 (37) ◽  
pp. 22556-22564
Author(s):  
Shiwen Wang ◽  
Guanjiang Liu ◽  
Bei Yang ◽  
Zifeng Zhang ◽  
Debo Hu ◽  
...  

We successfully achieved the direct detection of saliva glucose by a CNT-PEG-hydrogel. The top CNT-PEG layer provides channels for transporting glucose molecules and filters macromolecular impurities and the bottom base PBA-hydrogel film provides the glucose binding sites.


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