scholarly journals MONOMER STILBENOID GLUCOSIDES FROM Vatica pauciflora AND Vatica lowii (DIPTEROCARPACEAE)

2015 ◽  
Vol 77 (2) ◽  
Author(s):  
Aisyah Salihah Kamarozaman ◽  
Jalifah Latip ◽  
Christian Paetz ◽  
Yana M. Syah
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Room Temperature ◽  
Methanolic Extract ◽  
Pure Compound ◽  
Mass Spectral Data ◽  
Mass Spectral ◽  
Soxhlet Apparatus ◽  
Vacuum Liquid Chromatography ◽  
Phytochemical Studies

Phytochemical studies on the woods of Vatica pauciflora and Vatica lowii have been conducted. The woods of V. pauciflora were extracted with methanol by using Soxhlet apparatus while the woods of V. lowii were extracted with acetone at room temperature. The methanolic extract of V. pauciflora was semi-purified by using vacuum liquid chromatography (VLC) and further purified by high performance liquid chromatography (HPLC) to obtain a pure compound (1). The same technique of semi-purification has been employed on V. lowii acetone extract which was further purified by radial chromatography to give a pure compound (2). Based on the UV, IR, NMR and mass spectral data, the isolated compounds were characterized as (-)-cis-resveratrol-10-C-b-D-glucopyranoside and (-)-piceid respectively.

scholarly journals High Performance Thin Layer Chromatography Fingerprinting Analysis of Piper betle L. Leaves

2021 ◽  
pp. 8-15
Author(s):  
Ramdas N. Kale ◽  
Ravindra Y. Patil
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Piper Betle ◽  
Soxhlet Apparatus ◽  
Rf Values ◽  
Pharmacologically Active ◽  
Layer Chromatography

Introduction: Many modern medicines used today based on plants and plant products. Piper betle is generally known as the betle vine, it is an important medicinal and recreational plant. High performance thin layer chromatography (HPTLC) is an advanced powerful analytical method with more separation power, high performance and superior reproducibility than classic thin layer chromatography (TLC). A chromatographic fingerprint of a plant extract is a chromatographic pattern of some common chemical constituents of pharmacologically active and/or chemical characteristics. Chromatographic fingerprints are useful in authentication and identification of plant. Objectives:  Objectives of present research was to establish HPTLC fingerprinting of methanolic extract of Piper betle L. leaves. Materials and Methods: Methanolic extract of Piper betle leaves was prepared using soxhlet apparatus. HPTLC studies were performed using a CAMAG HPTLC system equipped with automatic TLC sampler-4 (ATS 4), TLC scanner 4, and vision CATS 3.0 software. Results: The study revealed the presence of alkaloids with Rf value 0.65, flavonoids with Rf values 0.19, 0.29, 0.72, 0.95., and phenolic compound with Rf value 0.7. Conclusion: The HPTLC fingerprinting profile developed for the methanolic extract of Piper betle L. leaves will help in proper identification of the plant.Piper betle

A ROBUST BIOMARKER DISCOVERY PIPELINE FOR HIGH-PERFORMANCE MASS SPECTROMETRY DATA

2007 ◽  
Vol 05 (05) ◽  
pp. 1023-1045 ◽  
Author(s):  
WAYNE G. FISHER ◽  
KEVIN P. ROSENBLATT ◽  
DAVID A. FISHMAN ◽  
GORDON R. WHITELEY ◽  
ALVYDAS MIKULSKIS ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Spectral Data ◽  
High Performance ◽  
Biomarker Discovery ◽  
Mass Spectrometry Data ◽  
Mass Spectral Data ◽  
Data Set ◽  
Mass Spectral ◽  
Peak Finding ◽  
Non Gaussian

A high-throughput software pipeline for analyzing high-performance mass spectral data sets has been developed to facilitate rapid and accurate biomarker determination. The software exploits the mass precision and resolution of high-performance instrumentation, bypasses peak-finding steps, and instead uses discrete m/z data points to identify putative biomarkers. The technique is insensitive to peak shape, and works on overlapping and non-Gaussian peaks which can confound peak-finding algorithms. Methods are presented to assess data set quality and the suitability of groups of m/z values that map to peaks as potential biomarkers. The algorithm is demonstrated with serum mass spectra from patients with and without ovarian cancer. Biomarker candidates are identified and ranked by their ability to discriminate between cancer and noncancer conditions. Their discriminating power is tested by classifying unknowns using a simple distance calculation, and a sensitivity of 95.6% and a specificity of 97.1% are obtained. In contrast, the sensitivity of the ovarian cancer blood marker CA125 is ~50% for stage I/II and ~80% for stage III/IV cancers. While the generalizability of these markers is currently unknown, we have demonstrated the ability of our analytical package to extract biomarker candidates from high-performance mass spectral data.

scholarly journals Portrayal of Punica granatum L. peel extract through High Performance Liquid Chromatography and antimicrobial activity evaluation

2023 ◽  
Vol 83 ◽  
Author(s):  
M.S. Mahmood ◽  
A. Ashraf ◽  
S. Ali ◽  
A.B. Siddique ◽  
F. Asad ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Punica Granatum ◽  
Diffusion Method ◽  
Staph Aureus ◽  
E Coli ◽  
Peel Extract

Abstract Increasing trend in antimicrobial resistance and failure of chemically synthesized antibiotics lead to discover alternative methods for the treatment of bacterial infections. Various medicinal plants are in use traditionally and their active compounds can be further applied for treatment of bacterial diseases. This study was designed to determine the antibacterial activity of Punica granatum (P. granatum L.) (pomegranate) peel extract against Enterobacteriaceae [Escherichia coli (E. coli), Salmonella Typhimurium (S. Typhimurium) and Shigella Dysenteriae (S. Dysenteriae)] and gram-positive bacterium [Staphylococcus aureus (Staph aureus)]. Methanolic extract of P. granatum L. peel was prepared by Soxhlet apparatus method. Total flavonoid and phenolic contents from the extract were determined by High Performance Liquid Chromatography (HPLC). The antibacterial activity of P. granatum L. peel extract was evaluated through agar well diffusion method. HPLC showed the range of phenolics (gallic acid, caffeic acid, benzoic acid, cinnamic acid) and flavonoid compounds. The chemical structures of flavonoid and phenolics found in the methanolic extract of P. granatum L. peel have been reported for the first time. The methanolic peel extract (50 ul) of yellow P. granatum L. showed 26, 10, 10 and 9mm zones of inhibition (ZOI) against S. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. The methanolic extract of red P. granatum L. (100 ul) showed 27, 8, 12 and 15 mm ZOI against Staph. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. Highest ZOI was observed against Staph. aureus. Many of the bacteria studied in the present work may cause serious gastrointestinal infections, which can lead to hemorrhagic diarrhea in children. These infections can be life-threatening to young children and the elderly. There is an incentive to find alternative control measures, such as plant and herbal extracts, especially in lesser-developed countries where traditional antibiotics may not be readily available.

scholarly journals Fungal Biotransformation of 6-Nitrochrysene

1998 ◽  
Vol 64 (8) ◽  
pp. 3106-3109 ◽  
Author(s):  
Jairaj V. Pothuluri ◽  
John B. Sutherland ◽  
James P. Freeman ◽  
Carl E. Cerniglia
Keyword(s):  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
High Performance ◽  
Environmental Contaminant ◽  
Cunninghamella Elegans ◽  
Spectral Techniques ◽  
Mass Spectral ◽  
Uv Visible

ABSTRACT The fungus Cunninghamella elegans was used to biotransform 6-nitrochrysene, a mutagen that is a widespread environmental contaminant. After 6 days, 74% of the3H-labeled 6-nitrochrysene added had been metabolized to two isomeric sulfate conjugates. These conjugates were separated by high-performance liquid chromatography and identified by UV-visible, 1H nuclear magnetic resonance, and mass spectral techniques as 6-nitrochrysene 1-sulfate and 6-nitrochrysene 2-sulfate.

Identification of an Impurity in Commercial Sources of the Tomato Glycoalkaloid Tomatine

1995 ◽  
Vol 78 (3) ◽  
pp. 691-692 ◽  
Author(s):  
Rodney J Bushway ◽  
Lewis B Perkins
Keyword(s):  
Liquid Chromatography ◽  
Double Bond ◽  
Spectral Data ◽  
Mass Spectroscopy ◽  
The Other ◽  
Equal Size ◽  
Mass Spectral Data ◽  
Mass Spectral ◽  
Data Support ◽  
Commercial Sources

Abstract Three commercial sources of tomatine showed 2 peaks of approximately equal size when determined by liquid chromatography (LC). Analysis by liquid chromatography/mass spectroscopy (LC/MS) showed one LC peak having an [M + H] ion at 1035, which corresponds to tomatine. The other peak had an [M + H] ion at 1033, which indicates a tomatine-like molecule that has tomatidenol for the aglycone instead of tomatidine or solasodine. Ultraviolet data support a delta C-5–C-6 double bond in the aglycone. LC of tomatine hydrolysate indicated the presence of tomatidine and tomatidenol and the absence of solasodine. Thus, the impurity was dehydrotomatine. Purity calculations based on mass spectral data showed that the tomatine standards were 80% pure.

scholarly journals FLAVONOIDS AND ANTI-OXIDANT ACTIVITY MEDIATED GASTROPROTECTIVE ACTION OF LEATHERY MURDAH, TERMINALIA CORIACEA (ROXB.) WIGHT & ARN. LEAF METHANOLIC EXTRACT IN RATS

2017 ◽  
Vol 54 (3) ◽  
pp. 183-191 ◽  
Author(s):  
Mohammed Safwan ALI KHAN ◽  
Shaaz NAZAN ◽  
Abdul Manan MAT JAIS
Keyword(s):  
Superoxide Dismutase ◽  
High Performance ◽  
Methanolic Extract ◽  
Ultra Violet ◽  
High Performance Liquid Chromatographic ◽  
Ulcer Index ◽  
Mass Spectral Analysis ◽  
Mass Spectral ◽  
Anti Oxidant ◽  
Liquid Chromatographic

ABSTRACT BACKGROUND Leathery Murdah, Terminalia coriacea (Roxb.) Wight & Arn. from family Combretaceae is used in Ayurveda and Siddha traditional systems of medicine to heal ulcers. OBJECTIVE The present study was conducted to assess the gastroprotective effect and understand the fundamental mechanism of action of Leathery Murdah, Terminalia coriacea (Roxb.) Wight & Arn. Leaf Methanolic Extract. METHODS The test extract was screened for anti-ulcer activity by Aspirin induced ulcerogenesis in pyloric ligation and ethanol induced gastric ulcers at three doses - 125, 250, and 500 mg/kg, p.o. using Ranitidine 50 mg/kg and Misoprostol 100 μg/kg as standard drug in respective models. Seven parameters were carefully examined, that is, ulcer index, total protein, mucin, catalase, malondialdehyde, and superoxide dismutase levels and histopathology. High Performance Liquid Chromatographic - Ultra Violet profiling and Liquid Chromatography - Mass Spectral analysis of crude Terminalia coriacea leaves methanolic extract were carried out as a part of chemical characterization to identify bioactive compounds. RESULTS All the test doses exhibited significant gastroprotective function, particularly the higher doses demonstrated improved action. The results revealed a significant increase in the levels of catalase, superoxide dismutase, and Mucin with reduction in ulcer index, the levels of total protein, and malondialdehyde. Histopathological observations also illustrated the gastroprotective effect of Terminalia coriacea leaves methanolic extract. CONCLUSION Terminalia coriacea leaves methanolic extract exhibited strong anti-oxidant and anti-secretory activities mediated gastroprotection besides inducing the gastric mucosal production. The observed pharmacological response can be attributed to the flavonoidal compounds namely - Quercetin-3-O-rutinoside, Luteolin-7-O-glucoside, Myricetin hexoside, Quercetin-3-O-glucoside, Isorhamnetin-3-O-rhamnosylglucoside and Isorhamnetin-3-O-glucoside identified in the extract for the first time with High Performance Liquid Chromatographic - Ultra Violet and Liquid Chromatography - Mass Spectral analysis.

scholarly journals Target Quantification and Semi-Target Screening of Undesirable Substances in Pear Juices Using Ultra-High-Performance Liquid Chromatography-Quadrupole Orbitrap Mass Spectrometry

Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 841
Author(s):  
Alfonso Narváez ◽  
Yelko Rodríguez-Carrasco ◽  
Luana Izzo ◽  
Luigi Castaldo ◽  
Alberto Ritieni
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Mean Value ◽  
Fusarium Mycotoxins ◽  
Mass Spectral ◽  
Pear Juice ◽  
Target Screening

Fruit juices are common products in modern diets due to the supply of vegetal nutrients combined with its tastiness. Nevertheless, potential contaminants, such as mycotoxins and pesticides, can be present in commercial products due to a potential carry-over. Therefore, the aim of this study was to investigate for the first time the presence of 14 Fusarium mycotoxins using a quick, easy, cheap, effective, rugged, and safe (QuEChERS)-based extraction followed by an ultra-high-performance liquid chromatography-quadrupole Orbitrap high-resolution mass spectrometry in 21 pear juice samples from Italian markets. Up to nine different mycotoxins were detected, particularly an extensive presence of zearalenone (67%, n = 21, mean value = 0.88 ng/mL). Emerging Fusarium mycotoxins enniatins B, B1, A, and A1 were also detected. Additionally, 77 pesticide residues were tentatively identified through a retrospective analysis based on a mass spectral library. The prevalent presence of some non-approved pesticides, such as ethoxyquin (64%, n = 21) and triazophos (55%, n = 21), must be highlighted. The results obtained indicate an extensive contamination of marketed pear juice with undesirable compounds, and they should be taken into consideration when performing risk assessment studies.

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