Isolation and Characterization of Metal and Antibiotic Resistant Psychrotrophic Bacteria from Refrigerated Spoiled Food

2014 ◽  
Vol 69 (1) ◽  
Author(s):  
Somayeh Asadi ◽  
Shafinaz Shahir ◽  
Zaharah Ibrahim ◽  
Adibah Yahya ◽  
Madihah Md Salleh ◽  
...  
Keyword(s):  
Metal Ions ◽  
Inhibitory Concentration ◽  
Metal Resistance ◽  
Antibiotic Resistant ◽  
Psychrotrophic Bacteria ◽  
Cold Environments ◽  
Isolation And Characterization ◽  
Identity Match ◽  
Microbial Strain

The aim of this study was to isolate and characterize psychrotrophic bacteria resistant to antibiotic and metals from cold samples of refrigerated spoiled food and ice. Two isolates named H and F were successfully isolated from samples incubated at 10°C and 4°C, respectively. Both isolates were able to grow at 4°C, 10°C, 20°C and 30°C and exhibited multidrug resistance to antibiotics (chloramphenicol, ampicillin, tetracycline and kanamycin). Bacteria H and F were then further tested for their resistance to metal ions such as Cd2+, Zn2+, Cr 6+ and As 3+   at concentrations ranging from 0-10 mM. The results of the test revealed that the minimum inhibitory concentration (MIC) for isolate H was determined at 2 mM for all the metal ions. By comparison, isolate F was highly resistant to Cr6+, Cd2+ andZn2+ with MIC of 10 mM for these cations, while MIC for As3+ was 4 mM. The results of comparing 16S rRNA gens demonstrated that isolate F has 98% identity match with Bacillus sp. This strain could be used as a model microbial strain to understand the mechanisms of metal resistance and as a source of psychrotrophic enzymes with potential application for bioremediation of heavy metals in cold environments.

Heavy-metal resistance in Gram-negative bacteria isolated from Kongsfjord, Arctic

2015 ◽  
Vol 61 (6) ◽  
pp. 429-435 ◽  
Author(s):  
C.S. Neethu ◽  
K.M. Mujeeb Rahiman ◽  
A.V. Saramma ◽  
A.A. Mohamed Hatha
Keyword(s):  
Heavy Metals ◽  
Inhibitory Concentration ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
The Arctic ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Isolation And Characterization ◽  
Tube Dilution

Isolation and characterization of heterotrophic Gram-negative bacteria was carried out from the sediment and water samples collected from Kongsfjord, Arctic. In this study, the potential of Arctic bacteria to tolerate heavy metals that are of ecological significance to the Arctic (selenium (Se), mercury (Hg), cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn)) was investigated. Quantitative assay of 130 isolates by means of plate diffusion and tube dilution methods was carried out by incorporation of different concentrations of metals. Growth in Se and Pb at a concentration of 3000 μg/L was significantly lower (P ≤ 0.0001) than at 2000 μg/L. The minimum inhibitory concentration for Cd and Hg was 50 μg/L (P ≤ 0.0001, F = 264.23 and P ≤ 0.0001, F = 291.08, respectively) even though in the tube dilution test, Hg-containing tubes showed much less growth, revealing its superior toxicity to Cd. Thus, the level of toxicity of heavy metals was found to be in the order of Hg > Cd > Cu > Zn > Pb > Se. Multiple-metal-resistant isolates were investigated for their resistance against antibiotics, and a positive correlation was observed between antibiotic and metal resistance for all the isolates tested. The resistant organisms thus observed might influence the organic and inorganic cycles in the Arctic and affect the ecosystem.

scholarly journals Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Saleem Farooq ◽  
Ruqeya Nazir ◽  
Shabir Ahmad Ganai ◽  
Bashir Ahmad Ganai
Keyword(s):  
Specific Activity ◽  
Temperature Range ◽  
Psychrotrophic Bacteria ◽  
Isolation And Characterization ◽  
Cold Active ◽  
Active Protease ◽  
Quantitative Screening ◽  
First Time ◽  
Low K

AbstractAs an approach to the exploration of cold-active enzymes, in this study, we isolated a cold-active protease produced by psychrotrophic bacteria from glacial soils of Thajwas Glacier, Himalayas. The isolated strain BO1, identified as Bacillus pumilus, grew well within a temperature range of 4–30 °C. After its qualitative and quantitative screening, the cold-active protease (Apr-BO1) was purified. The Apr-BO1 had a molecular mass of 38 kDa and showed maximum (37.02 U/mg) specific activity at 20 °C, with casein as substrate. It was stable and active between the temperature range of 5–35 °C and pH 6.0–12.0, with an optimum temperature of 20 °C at pH 9.0. The Apr-BO1 had low Km value of 1.0 mg/ml and Vmax 10.0 µmol/ml/min. Moreover, it displayed better tolerance to organic solvents, surfactants, metal ions and reducing agents than most alkaline proteases. The results exhibited that it effectively removed the stains even in a cold wash and could be considered a decent detergent additive. Furthermore, through protein modelling, the structure of this protease was generated from template, subtilisin E of Bacillus subtilis (PDB ID: 3WHI), and different methods checked its quality. For the first time, this study reported the protein sequence for psychrotrophic Apr-BO1 and brought forth its novelty among other cold-active proteases.

Isolation and characterization of antibiotic-resistant bacteria from pharmaceutical industrial wastewaters

2015 ◽  
Vol 89 ◽  
pp. 54-61 ◽  
Author(s):  
Leyla Tahrani ◽  
Leila Soufi ◽  
Ines Mehri ◽  
Afef Najjari ◽  
Abdenaceur Hassan ◽  
...  
Keyword(s):  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Industrial Wastewaters ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Bacterium isolated from Bantala Tannery Solid Wastes

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Anannya Banerjee ◽  
Sudipta Tripathi
Keyword(s):  
Inhibitory Concentration ◽  
West Bengal ◽  
Accession Number ◽  
Bacterial Strains ◽  
X Ray ◽  
Isolation And Characterization ◽  
Chrome Tanning ◽  
Tanning Process ◽  
Mic Minimum Inhibitory Concentration

Leather industries that uses the conventional chrome tanning process are subjected to high risk of contamination due to the emission of toxic Cr(VI) that poses a serious threat to the environment and human’s wellbeing. The present study were made to isolate and characterize chromium tolerant bacteria in the samples collected from four different plots of Bantala Tannery, Kolkata, West Bengal, India. Pure chromium tolerant bacterial strains were isolated from the tannery sludge samples and their relative MIC (Minimum Inhibitory Concentration) were recorded at different concentrations of Cr (VI) salts to select the highest chromium tolerant bacterium. The selected bacterium was further taken for their growth studies followed by different cultural, morphological and molecular analysis (16S rDNA). The bacterial strain was further studied through SEM (Scanning Electron microscopy) and EDX (Energy Dispersive X-ray) spectroscopy which revealed that TW4 was a gram positive, rod shaped, endospore forming, pleomorphic bacterium with phylogenetic similarities with Isoptericola sp. and genebank accession number SUB1732465 TW4 KX640927.

scholarly journals Isolation and Characterization of Antibiotic Resistant Bacteria from Swiftlet Feces in Swiftlet Farm Houses in Sarawak, Malaysia

2013 ◽  
Vol 7 (4) ◽  
pp. 137-143 ◽  
Author(s):  
LEONG SUI SIEN ◽  
SAMUEL LIHAN ◽  
LING TECK YEE ◽  
CHIA HWA CHUAN ◽  
LIM CHAN KOON
Keyword(s):  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of lytic phages TSE1-3 against Enterobacter cloacae

2016 ◽  
Vol 11 (1) ◽  
Author(s):  
Komal Ameer Khawaja ◽  
Zaigham Abbas ◽  
Shafiq ur Rehman
Keyword(s):  
Medical Practice ◽  
Enterobacter Cloacae ◽  
Therapeutic Agents ◽  
Alternative Therapies ◽  
Resistant Bacteria ◽  
Gene Products ◽  
Antibiotic Resistant ◽  
Isolation And Characterization ◽  
Alternative Approaches

AbstractThe emergence of antibiotic resistant bacterial pathogens is becoming a major challenge for patient care. The utilization of alternative therapies for infectious diseases other than antibiotics is an urgent need of today medical practice. The utilization of lytic bacteriophages and their gene products as therapeutic agents against antibiotic resistant bacteria is one of the convincing alternative approaches. Here we present the isolation and characterization of three lytic bacteriophages TSE1-3 against

scholarly journals Interaction of metal ions with d-glucobenzothiazoline: isolation and characterization of the resultant products

2002 ◽  
Vol 337 (4) ◽  
pp. 289-296 ◽  
Author(s):  
T.Mohan Das ◽  
Chebrolu P. Rao ◽  
Erkki Kolehmainen ◽  
Ramakant M. Kadam ◽  
Medury D. Sastry
Keyword(s):  
Metal Ions ◽  
Isolation And Characterization
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