The effects of necrostatin‐1 on the in vitro development and function of young porcine islets over 14‐day prolonged tissue culture

2021 ◽  
Author(s):  
Hien Lau ◽  
Nicole Corrales ◽  
Samuel Rodriguez ◽  
Soomin Park ◽  
Mohammadreza Mohammadi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
In Vitro Development ◽  
Porcine Islets ◽  
And Function ◽  
Vitro Development

scholarly journals Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets

2021 ◽  
Vol 22 (16) ◽  
pp. 8367
Author(s):  
Hien Lau ◽  
Shiri Li ◽  
Nicole Corrales ◽  
Samuel Rodriguez ◽  
Mohammadreza Mohammadi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oxygen Consumption ◽  
Insulin Secretion ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
In Vitro Development ◽  
Porcine Islets ◽  
Vitro Development

Pre-weaned porcine islets (PPIs) represent an unlimited source for islet transplantation but are functionally immature. We previously showed that necrostatin-1 (Nec-1) immediately after islet isolation enhanced the in vitro development of PPIs. Here, we examined the impact of Nec-1 on the in vivo function of PPIs after transplantation in diabetic mice. PPIs were isolated from pancreata of 8–15-day-old, pre-weaned pigs and cultured in media alone, or supplemented with Nec-1 (100 µM) on day 0 or on day 3 of culture (n = 5 for each group). On day 7, islet recovery, viability, oxygen consumption rate, insulin content, cellular composition, insulin secretion capacity, and transplant outcomes were evaluated. While islet viability and oxygen consumption rate remained high throughout 7-day tissue culture, Nec-1 supplementation on day 3 significantly improved islet recovery, insulin content, endocrine composition, GLUT2 expression, differentiation potential, proliferation capacity of endocrine cells, and insulin secretion. Adding Nec-1 on day 3 of tissue culture enhanced the islet recovery, proportion of delta cells, beta-cell differentiation and proliferation, and stimulation index. In vivo, this leads to shorter times to normoglycemia, better glycemic control, and higher circulating insulin. Our findings identify the novel time-dependent effects of Nec-1 supplementation on porcine islet quantity and quality prior to transplantation.

scholarly journals In Vitro Development of Bone Marrows from Patients with Neutropenia

Blood ◽  
1967 ◽  
Vol 29 (4) ◽  
pp. 462-468 ◽  
Author(s):  
PETER LAU ◽  
JEROME I. BRODY ◽  
LAWRENCE H. BEIZER
Keyword(s):  
Tissue Culture ◽  
Feedback Mechanism ◽  
The Body ◽  
In Vitro Development ◽  
Normal Bone ◽  
Vitro Development

Abstract Bone marrows from patients with neutropenia, characterized morphologically by a paucity of mature neutrophils, underwent normal maturation when removed from the body and grown in tissue culture. In addition, certain leukopenic plasmas appeared to stimulate development of normal bone marrows under similar circumstances. These observations suggest that the granulocytic elements in benign forms of neutropenia are innately capable of normal sequential growth and that sustained peripheral neutropenia may distort the normal feedback mechanism which regulates leukopoiesis.

In vitro development of human triploid zygotes reconstructed by pronuclear transfer

2002 ◽  
Vol 78 ◽  
pp. S180-S181
Author(s):  
John Zhang ◽  
Yi Ming Shu ◽  
Lewis C Krey ◽  
Hui Liu ◽  
Guang Lun Zhuang ◽  
...  
Keyword(s):  
In Vitro Development ◽  
Pronuclear Transfer ◽  
Vitro Development

Gallic acid promotes the in vitro development of sheep secondary isolated follicles involving the phosphatidylinositol 3-kinase pathway

2021 ◽  
pp. 106767
Author(s):  
Gizele A.L. Silva ◽  
Luana B. Araújo ◽  
Larissa C.R. Silva ◽  
Bruna B. Gouveia ◽  
Ricássio S. Barberino ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Phosphatidylinositol 3 Kinase ◽  
In Vitro Development ◽  
Vitro Development ◽  
Kinase Pathway ◽  
Phosphatidylinositol 3

In vitro development of plants from microspores of rice

In Vitro ◽  
1977 ◽  
Vol 13 (8) ◽  
pp. 484-489 ◽  
Author(s):  
Chi-Chang Chen
Keyword(s):  
In Vitro Development ◽  
Vitro Development
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