Immortalization of porcine hepatocytes with a α‐1,3‐galactosyltransferase knockout background

2019 ◽  
Vol 27 (1) ◽  
Author(s):  
Quancheng Wang ◽  
Xuan Zhang ◽  
Bo Wang ◽  
Ge Bai ◽  
Dengke Pan ◽  
...  
Keyword(s):  
Porcine Hepatocytes

scholarly journals Transplantation of Porcine Hepatocytes Cultured with Polylactic Acid-O-Carboxymethylated Chitosan Nanoparticles Promotes Liver Regeneration in Acute Liver Failure Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Zhong Chen ◽  
Renan Chang ◽  
Weijun Guan ◽  
Hongyu Cai ◽  
Fei Tang ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Chitosan Nanoparticles ◽  
Collagen Gel ◽  
Post Transplantation ◽  
Ki 67 ◽  
Group A ◽  
Group B ◽  
Group D ◽  
High Liver ◽  
Porcine Hepatocytes

In this study, free porcine hepatocytes suspension (Group A), porcine hepatocytes embedded in collagen gel (Group B), porcine hepatocytes cultured with PLA-O-CMC nanoparticles and embedded in collagen gel (Group C), and PLA-O-CMC nanoparticles alone (Group D) were transplanted into peritoneal cavity of ALF rats, respectively. The result showed that plasma HGF levels were elevated post-transplantation with a peak at 12 hr. The rats in Group C showed highest plasma HGF levels at 2, 6, 12, 24 and 36 hr post-transplantation and lowest HGF level at 48 hr. Plasma VEGF levels were elevated at 48 hr post-transplantation with a peak at 72 hr. The rats in Group C showed highest plasma HGF levels at 48, 72, and 96 hr post-transplantation. The liver functions in Group C were recovered most rapidly. Compared with Group B, Group C had significant high liver Kiel 67 antigen labeling index (Ki-67 LI) at day 1 post-HTx (P<.05). Ki-67 LI in groups B and C was higher than that in groups A and D at days 5 and 7 post-HTx. In conclusion, intraperitoneal transplantation of porcine hepatocytes cultured with PLA-O-CMC nanoparticles and embedded in collagen gel can promote significantly liver regeneration in ALF rats.

Automated Liver Cell Processing Facilitates Large Scale Isolation and Purification of Porcine Hepatocytes

ASAIO Journal ◽  
1995 ◽  
Vol 41 (2) ◽  
pp. 155-161
Author(s):  
Eugenio Morsiani ◽  
Jacek Rozga ◽  
Henri C. Scott ◽  
Laura T. Lebow ◽  
Albert D. Moscioni ◽  
...  
Keyword(s):  
Liver Cell ◽  
Large Scale ◽  
Isolation And Purification ◽  
Cell Processing ◽  
Porcine Hepatocytes

scholarly journals Diquat Determines a Deregulation of lncRNA and mRNA Expression in the Liver of Postweaned Piglets

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Jin Wang ◽  
Zhi-xin Li ◽  
Dan-dan Yang ◽  
Pei-qi Liu ◽  
Zhi-qiang Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Growth Performance ◽  
Signaling Pathways ◽  
Molecular Mechanisms ◽  
Reduction Process ◽  
Future Research ◽  
Control Group ◽  
Oxidoreductase Activity ◽  
Go Terms ◽  
Porcine Hepatocytes

Oxidative stress is detrimental to animals and can depress the growth performance and regulate the gene expression of animals. However, it remains unclear how oxidative stress regulates the expression of long noncoding RNAs (lncRNAs) and mRNAs. Therefore, the purpose of this article was to explore the profiles of lncRNAs and mRNAs in the liver of piglets under oxidative stress. Here, we constructed a piglet oxidative stress model induced by diquat and evaluated the effects of oxidative stress on the growth performance and antioxidant enzyme activity of piglets. We also used RNA-Seq to examine the global expression of lncRNAs and mRNAs in piglets under oxidative stress. The targets of lncRNAs and mRNAs were enriched in gene ontology (GO) terms and signaling pathways. The results show that the growth performance and activities of antioxidant enzymes were decreased in piglets under oxidative stress. Moreover, eight lncRNAs (6 upregulated and 2 downregulated) and 30 mRNAs (8 upregulated and 22 downregulated) were differentially expressed in the oxidative stress group of piglets compared to the negative control group. According to biological processes in enriched GO terms, the oxoacid metabolic process, intramolecular oxidoreductase activity, and oxidation-reduction process play important roles in oxidative stress. Pathway analysis showed that the signaling pathways involved in insulin and glucose metabolism had a close relationship with oxidative stress. Furtherin vitroexperiments showed that the expression of the upregulated geneGNMTwas significantly increased in primary porcine hepatocytes after diquat stimulation. In contrast, the level of the downregulated geneGCKwas significantly decreased at 12 h in primary porcine hepatocytes after diquat stimulation. Our results expand our knowledge of the lncRNAs and mRNAs transcribed in the livers of piglets under oxidative stress and provide a basis for future research on the molecular mechanisms mediating oxidative stress and tissue damage.

Xenotransplantation of isolated encapsulated porcine hepatocytes in the treatment of a highly fulminant hepatic failure Model

1997 ◽  
Vol 29 (1-2) ◽  
pp. 920-922 ◽  
Author(s):  
P.A. Rivas-Vetencourt ◽  
E.D. Aranda ◽  
L. Sorio ◽  
Z. Quero ◽  
A. Martinez ◽  
...  
Keyword(s):  
Fulminant Hepatic Failure ◽  
Hepatic Failure ◽  
Failure Model ◽  
Porcine Hepatocytes

Functional Recovery of Porcine Hepatocytes after Hypothermic or Cryogenic Preservation for Liver Support Systems

1997 ◽  
Vol 6 (5) ◽  
pp. 447-454 ◽  
Author(s):  
Sharda Naik ◽  
Henry A. Santangini ◽  
Donna M. Trenkler ◽  
Claudy J.P. Mullon ◽  
Barry A. Solomon ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Culture Media ◽  
Cost Effective ◽  
Isolated Hepatocytes ◽  
Specific Cell ◽  
Isolated Cells ◽  
Liver Support ◽  
C Storage ◽  
Liver Assist Devices ◽  
Porcine Hepatocytes

The provision of an immediate supply of isolated porcine hepatocytes for artificial liver support requires preservation techniques that will allow maintenance of cell viability and detoxification functions. By means of a simple and cost-effective cryopreservation system, porcine hepatocytes can be available for both local and distant medical treatment facilities. Additionally, cryopreservation provides an adequate period for quality control testing to be completed prior to use of any specific cell lot. We are reporting a dual approach, namely the preservation of porcine hepatocytes, at 4°C and at −196°C in liquid nitrogen (LN2). Using a combination of cryoprotectant agents with Chee's modified Eagle's culture media (CEM), collagenase isolated hepatocytes stored at 4°C for 24 h maintained 80% of the initial diazepam metabolism measured in freshly isolated cells and nearly 100% of initial function was preserved in hepatocytes stored up to 6 mo at -196°C. University of Wisconsin solution (UW) was also tested and while adequate for 4°C storage, it certainly did not match the performance of the CEM formulations for preservation of metabolic function of cells stored in liquid nitrogen. Based on our results of viability and detoxification function the combination of CEM with DMSO, polyethylene glycol and serum provided optimal protection for LN2 frozen cells. Other findings in these studies underlined the importance of the gradual introduction of DMSO in the prefreezing process, the period of osmotic equilibration, and the rapid postthaw withdrawal of this agent to minimize cytotoxic effects at these critical stages. Our freezing methodology provides the foundation for further technological developments in the cryopreservation of the large numbers of cells (billions) that are necessary for extracorporeal liver assist devices.

High-Yield Preparation of Porcine Hepatocytes for Long Survival after Transplantation in the Spleen

1985 ◽  
Vol 17 (6) ◽  
pp. 377-382 ◽  
Author(s):  
B. Nordlinger ◽  
M.E. Bouma ◽  
S.R. Wang ◽  
F. Ballet ◽  
N. Verthier ◽  
...  
Keyword(s):  
High Yield ◽  
Long Survival ◽  
Porcine Hepatocytes

Culture of Porcine Hepatocytes: The Dogma of Exogenous Matrix Revisited

2001 ◽  
Vol 25 (7) ◽  
pp. 546-550 ◽  
Author(s):  
Alicia Lorenti ◽  
Mariana Barbich ◽  
Alejandra Hidalgo ◽  
Sung Ho Hyon ◽  
Patricia Sorroche ◽  
...  
Keyword(s):  
Porcine Hepatocytes

Radial flow bioreactor outperforms hollow-fiber modules as a perfusing culture system for primary porcine hepatocytes

2000 ◽  
Vol 32 (8) ◽  
pp. 2715-2718 ◽  
Author(s):  
E. Morsiani ◽  
D. Galavotti ◽  
A.C. Puviani ◽  
L. Valieri ◽  
M. Brogli ◽  
...  
Keyword(s):  
Hollow Fiber ◽  
Culture System ◽  
Radial Flow ◽  
Radial Flow Bioreactor ◽  
Porcine Hepatocytes
Sign in / Sign up

Export Citation Format

Share Document