Long‐term culture and in vitro maturation of macroencapsulated adult and neonatal porcine islets

2018 ◽  
Vol 26 (2) ◽  
pp. e12461 ◽  
Author(s):  
Nizar I. Mourad ◽  
Pierre Gianello
Keyword(s):  
In Vitro Maturation ◽  
Long Term Culture ◽  
Porcine Islets ◽  
Neonatal Porcine Islets

In Vitro Maturation of Neonatal Porcine Islets

2006 ◽  
Vol 944 (1) ◽  
pp. 47-61 ◽  
Author(s):  
TANYA M. BINETTE ◽  
JANNETTE M. DUFOUR ◽  
GREGORY S. KORBUTT
Keyword(s):  
In Vitro Maturation ◽  
Porcine Islets ◽  
Neonatal Porcine Islets

Evaluation of cortical granules and viability of canine oocytes during long-term in vitro maturation

2007 ◽  
Vol 160 (6) ◽  
pp. 196-198 ◽  
Author(s):  
M. De los Reyes ◽  
J. Palomino ◽  
S. Sepulveda ◽  
R. Moreno ◽  
V. Parraguez ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Cortical Granules

scholarly journals Evaluation of amniotic hydrogel’s impact on the development of murine pre-antral follicles in mouse model

2019 ◽  
Vol 2 (4) ◽  
pp. 32-39
Author(s):  
Van Ngoc Le Trinh ◽  
Hang Thi Kim Tran ◽  
Thu Thuy Anh Vo ◽  
Tuyet Thi Vi Le ◽  
Ha Le Bao Tran
Keyword(s):  
In Vitro Maturation ◽  
High Demand ◽  
Antral Follicles ◽  
Secondary Follicle ◽  
The Moment ◽  
Main Component ◽  
Supporting Frame ◽  
Mature Process

Global average infertility rate is about 6– 12%, and in Vietnam at around 7.7%. As a result, there is a high demand for treatment, especially for female infertility. In vitro maturation (IVM) was evaluated and proven to be the most popular and promising at the moment. In long-term cultivation, the follicle was observed to extend, therefore, the usage of a supporting frame is quite necessary to maintain follicle’s natural sphere structure as well as completing the mature process. Amniotic membrane is an avascular membrane, composed of collagen, fibronectin, nidogen, proteoglycan, containing a big number of growth – factors with antimicrobial, anti-inflammatory, low immunogenicity and viscoelasticity properties. Amniotic hydrogel owns structure formed with thin fibers to help preserve the main component as collagen, which can turn to gel form at 37 degree Celsius. With those properties, amniotic hydrogel showed high potential as a scaffold for the follicle. When amniotic hydrogel is used as a scaffold for cultivating of secondary follicle (100 – 130 µm), the size of oocyte and follicle increased after 12 days of culturing, along with the formation of antrum. The results demonstrated the possibility to use amniotic hydrogel as a scaffold for the development of the secondary follicle.

scholarly journals Loss of primitive hematopoietic progenitors in patients with human immunodeficiency virus infection

Blood ◽  
1996 ◽  
Vol 88 (12) ◽  
pp. 4568-4578 ◽  
Author(s):  
A Marandin ◽  
A Katz ◽  
E Oksenhendler ◽  
M Tulliez ◽  
F Picard ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Human Immunodeficiency Virus ◽  
Long Term Culture ◽  
Cd34 Cells ◽  
Immunodeficiency Virus ◽  
Cell Fractions ◽  
Cd38 Antigen ◽  
Hiv 1

A number of hematologic abnormalities, including cytopenias, have been observed in patients with human immunodeficiency virus (HIV) infection. To elucidate their mechanisms, primitive cells from bone marrow aspirates of 21 patients with HIV-1 infection were quantitated by flow cytometry. The mean percentage of CD34+ cells is not significantly altered in HIV-1-infected patients in comparison with HIV-1- seronegative controls. In contrast, two- and three-color immunofluorescence analysis showed that in all HIV-1 samples, most CD34+ cells coexpressed the CD38 antigen. The proportion of HIV-1- derived CD34+ cells that did not express the CD38 antigen was significantly lower (HIV-1+: mean, 1.73%; controls: mean, 14%; P < .0005) than in controls. Moreover, of Thy-1+ cells, the proportion of CD34+ cells was twofold lower in HIV-1-infected patients (HIV-1+: mean, 12%; controls, 25%, P < .0005), which suggests that phenotypically primitive cells are depleted in HIV-1 infection. In vitro functional analysis in long-term cultures of sorted CD34+ cells from seven HIV-1 patients showed that CD34+ cells from HIV-1 patients generated much fewer colonies both in the nonadherent and adherent layers than CD34+ cells from controls after 5 weeks of culture (10-fold and four-fold less, respectively). Precise long-term culture initiating cell (LTC-IC) frequency in the CD34+ cell population was determined in three patients by limiting dilution and was markedly decreased in comparison to that of normal controls (from twofold to > sevenfold decreased). To determine if primitive cells were infected by HIV-1, both methylcellulose colonies generated from long-term culture of CD34+ cells and various CD34+ cell fractions purified by flow cytometry were evaluated for the presence of HIV-1 by polymerase chain reaction (PCR). Progeny from long-term culture was HIV-1-negative in three samples. In addition, using a sensitive PCR technique, the HIV-1 genome could not be detected in CD34+, CD34+/CD38-, and CD34+/CD4+ cells. These data show that hematologic disorders in HIV disease may be the consequence of a deficit of primitive cells. However, direct infection of these cells by HIV-1 does not seem to be responsible for this defect.

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