Fibronectin‐derived Epiviosamines enhance PDGF‐BB‐stimulated human dermal fibroblast migration in vitro and granulation tissue formation in vivo

2019 ◽  
Vol 27 (6) ◽  
pp. 634-649 ◽  
Author(s):  
Atulya Prasad ◽  
Fubao Lin ◽  
Richard A. F. Clark
Keyword(s):  
Granulation Tissue ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Tissue Formation ◽  
Granulation Tissue Formation ◽  
Fibroblast Migration

A novel design of wound bandage using heparin-polyvinylpyrrolidone/TiO2 nanocomposite to improved antibacterial treatment and burn wound healing effect: In vitro and in vivo evaluation

2021 ◽  
Vol 11 (11) ◽  
pp. 1808-1818
Author(s):  
Xiuli Li ◽  
Jigang Wang ◽  
Xin Li ◽  
Xiaoqian Hou ◽  
Hao Wang ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Dermal Fibroblast ◽  
Synergistic Effects ◽  
Bacterial Species ◽  
Human Dermal Fibroblast ◽  
Burn Wound ◽  
In Vivo Evaluation ◽  
Closure Rate

In our current study, porous heparin-polyvinylpyrrolidone/TiO2 nanocomposite (HpPVP/TiO2) bandage were prepared via the incorporation of TiO2 into HpPVP hydrogels for biomedical applications such as burn infection. The effect of the HpPVP hydrogels and the nanoparticles of TiO2 composition on the functional group and the surface properties of the as-fabricated bandages were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray diffractometry (XRD). The presence of TiO2 nanoparticles created the internal structure of the HpPVP hydrogel that aids in a homogeneous porous structure, as indicated by the scanning electron microscope (SEM). The size distribution of the TiO2 nanoparticles was measured using a transmission electron microscope (TEM). The studies on the mechanical properties of the HpPVP hydrogel indicate that the addition of TiO2 nanoparticles increases its strength. The prepared HpPVP/TiO2 nanocomposite dressing has excellent antimicrobial activity were tested against bacterial species (Staphylococcus aureus and Escherichia coli) and has good biocompatibility against human dermal fibroblast cells (HFFF2) for biological applications. In addition, in vivo evaluations in Kunming mice exposed that the as-fabricated HpPVP/TiO2 nanocomposite bandages increased the wound curing and facilitated accelerate skin cell construction along with collagen development. The synergistic effects of the HpPVP/TiO2 nanocomposite hydrogel dressing material, such as its excellent hydrophilic nature, good bactericidal activity, biocompatibility and wound closure rate through in vivo test makes it a suitable candidate for burn infections.

047 rAd?p21 Significantly Attenuates Granulation Tissue Formation and Scar Thickness In Vivo

2004 ◽  
Vol 12 (2) ◽  
pp. A14-A14 ◽  
Author(s):  
D‐L. Gu ◽  
I. Atencio ◽  
D. Looper ◽  
D.W. Kang ◽  
D. Maneval ◽  
...  
Keyword(s):  
Granulation Tissue ◽  
Tissue Formation ◽  
Granulation Tissue Formation

Overexpression of hepatocyte growth factor/scatter factor promotes vascularization and granulation tissue formation in vivo

FEBS Letters ◽  
2001 ◽  
Vol 509 (1) ◽  
pp. 95-100 ◽  
Author(s):  
Mitsuo Toyoda ◽  
Hisashi Takayama ◽  
Norio Horiguchi ◽  
Toshiyuki Otsuka ◽  
Toshio Fukusato ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Granulation Tissue ◽  
Tissue Formation ◽  
Scatter Factor ◽  
Granulation Tissue Formation ◽  
Hepatocyte Growth

The connexin mimetic peptide Gap27 increases human dermal fibroblast migration in hyperglycemic and hyperinsulinemic conditions in vitro

2011 ◽  
Vol 227 (1) ◽  
pp. 77-87 ◽  
Author(s):  
Catherine S. Wright ◽  
Simone Pollok ◽  
David J. Flint ◽  
Johanna M. Brandner ◽  
Patricia E.M. Martin
Keyword(s):  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Mimetic Peptide ◽  
Fibroblast Migration

scholarly journals In Vitro Expansion of Keratinocytes on Human Dermal Fibroblast-Derived Matrix Retains Their Stem-Like Characteristics

2018 ◽  
Author(s):  
Chee-Wai Wong ◽  
Beverley F. Kinnear ◽  
Radoslaw M. Sobota ◽  
Rajkumar Ramalingam ◽  
Catherine F. LeGrand ◽  
...  
Keyword(s):  
Cell Fate ◽  
Dermal Fibroblast ◽  
Human Keratinocytes ◽  
Human Dermal Fibroblast ◽  
Dermal Fibroblasts ◽  
Primary Human Keratinocytes ◽  
Matrix Deposition ◽  
Forming Efficiency

SummaryThe long-term expansion of keratinocytes under serum- and feeder free conditions generally results in diminished proliferation and an increased commitment to terminal differentiation. Here we present a serum and xenogeneic feeder free culture system that retains the self-renewal capacity of primary human keratinocytes. In vivo, the tissue microenvironment is a major contributor to determining cell fate and a key component of the microenvironment is the extracellular matrix (ECM). Accordingly, acellular ECMs derived from human dermal fibroblasts, cultured under macromolecular crowding conditions to facilitate matrix deposition and organisation, were used as the basis for a xenogeneic-free keratinocyte expansion protocol. A phospholipase A2 decellularisation procedure produced matrices which, by proteomics analysis, resembled in composition the core matrix proteins of skin dermis. On these ECMs keratinocytes proliferated rapidly, retained their small size, expressed p63, did not express keratin 10 and rarely expressed keratin 16. Moreover, the colony forming efficiency of keratinocytes cultured on these acellular matrices was markedly enhanced. Collectively these data indicate that the dermal fibroblast-derived matrices support the in vitro expansion of keratinocytes that maintained stem-like characteristics under serum free conditions.

Biological and biomedical applications of collagenous biomaterials

1990 ◽  
Vol 48 (3) ◽  
pp. 856-857
Author(s):  
Yasushi P. Kato ◽  
Michael G. Dunn ◽  
Frederick H. Silver ◽  
Arthur J. Wasserman
Keyword(s):  
Biomedical Applications ◽  
Soft Tissues ◽  
Collagen Fibers ◽  
Bone Collagen ◽  
Cover Slip ◽  
Fibroblast Migration ◽  
Cellular Expression ◽  
Defect Repair

Collagenous biomaterials have been used for growing cells in vitro as well as for augmentation and replacement of hard and soft tissues. The substratum used for culturing cells is implicated in the modulation of phenotypic cellular expression, cellular orientation and adhesion. Collagen may have a strong influence on these cellular parameters when used as a substrate in vitro. Clinically, collagen has many applications to wound healing including, skin and bone substitution, tendon, ligament, and nerve replacement. In this report we demonstrate two uses of collagen. First as a fiber to support fibroblast growth in vitro, and second as a demineralized bone/collagen sponge for radial bone defect repair in vivo.For the in vitro study, collagen fibers were prepared as described previously. Primary rat tendon fibroblasts (1° RTF) were isolated and cultured for 5 days on 1 X 15 mm sterile cover slips. Six to seven collagen fibers, were glued parallel to each other onto a circular cover slip (D=18mm) and the 1 X 15mm cover slip populated with 1° RTF was placed at the center perpendicular to the collagen fibers. Fibroblast migration from the 1 x 15mm cover slip onto and along the collagen fibers was measured daily using a phase contrast microscope (Olympus CK-2) with a calibrated eyepiece. Migratory rates for fibroblasts were determined from 36 fibers over 4 days.

scholarly journals Bilateral Choanal Atresia and Endoscopic Surgery: A Chance for CHARGE Patients

2021 ◽  
Vol 10 (13) ◽  
pp. 2951
Author(s):  
Maria Baldovin ◽  
Diego Cazzador ◽  
Claudia Zanotti ◽  
Giuliana Frasson ◽  
Athanasios Saratziotis ◽  
...  
Keyword(s):  
Congenital Malformation ◽  
Endoscopic Surgery ◽  
Granulation Tissue ◽  
Choanal Atresia ◽  
Tissue Formation ◽  
Endoscopic Repair ◽  
Granulation Tissue Formation ◽  
Charge Association ◽  
Cohort Data

Bilateral choanal atresia (CA) is a rare congenital malformation frequently associated with other anomalies. CHARGE association is closely linked to bilateral CA. The aim of this study was to describe the outcomes of the endoscopic repair in bilateral CA, and to assess the role of postoperative nasal stenting in two cohorts of CHARGE-associated and non-syndromic CA. Thirty-nine children were retrospectively analyzed (16 patients had CHARGE-associated CA). The rate of postoperative neochoanal restenosis was 31.3% in the CHARGE population, and 47.8% in the non-syndromic CA cohort. Data on postoperative synechiae and granulation tissue formation, need for endonasal toilette and dilation procedures, and number of procedures per patient were presented. Stent positioning led to a higher number of postoperative dilation procedures per patient in the non-syndromic cohort (p = 0.018), and to a higher rate of restenosis both in the CHARGE-associated, and non-syndromic CA populations. Children with CHARGE-associated and non-syndromic bilateral CA benefitted from endonasal endoscopic CA correction. The postoperative application of an endonasal stent should be carefully evaluated.

In Vitro Degradation of β-TCP/PLLA Scaffolds with Different Proportions of β-TCP

2007 ◽  
Vol 336-338 ◽  
pp. 1545-1548
Author(s):  
Lin Luo ◽  
Guang Fu Yin ◽  
Yun Zhang ◽  
Ya Dong Yao ◽  
Wei Zhong Yang ◽  
...  
Keyword(s):  
Degradation Rate ◽  
Porous Scaffolds ◽  
Carbonate Apatite ◽  
Tissue Formation ◽  
Cellular Attachment ◽  
Biodegradable Scaffolds ◽  
Scaffold Structure ◽  
Mechanical Strengths

Porous biodegradable scaffolds are widely used in bone tissue engineering to provide temporary templates for cellular attachment and matrix synthesis. Ideally, the degradation rate in vivo may be similar or slightly less than that of tissue formation, allowing for the maintenance of the scaffold structure and the mechanical support during early stages of tissue formation. Eventually, the 3-D spaces occupied by the porous scaffolds will be replaced by newly formed tissue. In this work, β-tricalcium phosphate/Poly-L lactide (β-TCP/PLLA) scaffolds with different proportions of β-TCP to PLLA were investigated. The effects of β-TCP proportions on degradation rate and mechanical strengths of the scaffolds were evaluated in simulated body fluid (SBF) at 37°C up to 42 days. Results show that: different proportions of β-TCP to PLLA have significant influence on degradation behaviors of the scaffolds, and mechanical strengths of the scaffolds with weight proportion of β-TCP to PLLA being 2 to 1 are much higher than those of the others during the degradation period. And in this period, the scaffolds biodegrade slowly, and Hydroxyl Carbonate Apatite (HCA) forms in the surface of the material.

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