Inherent variations in the cellular events at the site of amputation orchestrate scar‐free wound healing in the tail and scarred wound healing in the limb of lizard Hemidactylus flaviviridis

2018 ◽  
Vol 26 (5) ◽  
pp. 366-380 ◽  
Author(s):  
Isha Ranadive ◽  
Sonam Patel ◽  
Pranav Buch ◽  
Gowrikumari Uggini ◽  
Isha Desai ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cellular Events ◽  
Hemidactylus Flaviviridis

Ascorbic acid in the normal and regenerating tail of the house lizard, Hemidactylus flaviviridis

Development ◽  
1971 ◽  
Vol 26 (2) ◽  
pp. 285-293
Author(s):  
R. V. Shah ◽  
P. K. Hiradhar ◽  
D. K. Magon
Keyword(s):  
Wound Healing ◽  
Ascorbic Acid ◽  
Matrix Material ◽  
Growth Period ◽  
Tail Regeneration ◽  
The Matrix ◽  
Metabolic Activities ◽  
Vitamin Level ◽  
Hemidactylus Flaviviridis

The concentration of ascorbic acid (AA) and the histochemical distribution of the vitamin in the normal and regenerating tail of the gekkonid lizard, Hemidactylus flaviviridis, have been investigated. In the regenerating tail of the lizard the AA concentration almost doubles during wound healing and becomes fivefold during differentiation. However, it falls almost to the normal level during the blastema phase (i.e. period between wound healing and differentiation). Again, during the growth period (i.e. after differentiation) the AA concentration gradually becomes reduced, reaching the normal mark as the regenerate regains the full length of the original tail. Nevertheless, the vitamin level does not fall below the normal mark at any stage of regeneration. Increase of ascorbic acid during wound healing is thought to be mainly due to increased demand for the vitamin at the broken ends of the stump tissues, for their repair and formation of wound epithelium; the vitamin is known to help these processes. A fivefold increase of the vitamin during the differentiation period corresponds to an increased pace of laying down of the matrix material for the connective tissues, suggesting the role of ascorbic acid in the formation of collagen and mucopolysaccharides. Besides, the role of ascorbic acid in lipid and carbohydrate metabolism is also important during tail regeneration. Fluctuations in the vitamin level during different phases of tail regeneration are correlated with various states of metabolic activities of the corresponding phases.

scholarly journals Wound Healing and Omega-6 Fatty Acids: From Inflammation to Repair

2018 ◽  
Vol 2018 ◽  
pp. 1-17 ◽  
Author(s):  
Jéssica R. Silva ◽  
Beatriz Burger ◽  
Carolina M. C. Kühl ◽  
Thamiris Candreva ◽  
Mariah B. P. dos Anjos ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Wound Healing ◽  
Current Knowledge ◽  
Phagocytic Capacity ◽  
Cellular Events ◽  
Species Survival ◽  
Evolutionarily Conserved ◽  
Omega 6 ◽  
Cell Migration And Proliferation

Wound healing is an evolutionarily conserved process that is essential for species survival. Wound healing involves a series of biochemical and cellular events that are tightly controlled, divided into 3 concomitant and overlapping phases: inflammation, proliferation, and remodelling. Poor wound healing or a chronic wound represents a silent epidemic that affects billions of people worldwide. Considering the involvement of immune cells in its resolution, recent studies are focused on investigating the roles of immune nutrients such as amino acids, minerals, and fatty acids on wound healing. Among the fatty acids, much attention has been given to omega-6 (ω-6) fatty acids since they can modulate cell migration and proliferation, phagocytic capacity, and production of inflammatory mediators. The present review summarizes current knowledge about the role of ω-6 fatty acids in the wound healing context.

Nerve growth factor accelerates the early cellular events associated with wound healing

1985 ◽  
Vol 43 (2) ◽  
pp. 274-281 ◽  
Author(s):  
Michael J.P. Lawman ◽  
Michael D.P. Boyle ◽  
Adrian P. Gee ◽  
Michael Young
Keyword(s):  
Wound Healing ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Cellular Events

scholarly journals An Entropy-Based Automated Cell Nuclei Segmentation and Quantification: Application in Analysis of Wound Healing Process

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Varun Oswal ◽  
Ashwin Belle ◽  
Robert Diegelmann ◽  
Kayvan Najarian
Keyword(s):  
Wound Healing ◽  
Probability Distributions ◽  
Healing Process ◽  
Input Image ◽  
Wound Healing Process ◽  
Threshold Values ◽  
Cell Nuclei ◽  
Nuclei Segmentation ◽  
Cellular Events ◽  
Histological Images

The segmentation and quantification of cell nuclei are two very significant tasks in the analysis of histological images. Accurate results of cell nuclei segmentation are often adapted to a variety of applications such as the detection of cancerous cell nuclei and the observation of overlapping cellular events occurring during wound healing process in the human body. In this paper, an automated entropy-based thresholding system for segmentation and quantification of cell nuclei from histologically stained images has been presented. The proposed translational computation system aims to integrate clinical insight and computational analysis by identifying and segmenting objects of interest within histological images. Objects of interest and background regions are automatically distinguished by dynamically determining 3 optimal threshold values for the 3 color components of an input image. The threshold values are determined by means of entropy computations that are based on probability distributions of the color intensities of pixels and the spatial similarity of pixel intensities within neighborhoods. The effectiveness of the proposed system was tested over 21 histologically stained images containing approximately 1800 cell nuclei, and the overall performance of the algorithm was found to be promising, with high accuracy and precision values.

scholarly journals Collagen-Containing Fish Sidestream-Derived Protein Hydrolysates Support Skin Repair via Chemokine Induction

Marine Drugs ◽  
2021 ◽  
Vol 19 (7) ◽  
pp. 396
Author(s):  
Ioanna Lapi ◽  
Ourania Kolliniati ◽  
Tone Aspevik ◽  
Eleftherios E. Deiktakis ◽  
Konstantinos Axarlis ◽  
...  
Keyword(s):  
Wound Healing ◽  
Protein Hydrolysate ◽  
Repair Process ◽  
Protein Hydrolysates ◽  
Angiogenic Factor ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Cellular Events ◽  
Skin Repair ◽  
Health Promoting

Restoring homeostasis following tissue damage requires a dynamic and tightly orchestrated sequence of molecular and cellular events that ensure repair and healing. It is well established that nutrition directly affects skin homeostasis, while malnutrition causes impaired tissue healing. In this study, we utilized fish sidestream-derived protein hydrolysates including fish collagen as dietary supplements, and investigated their effect on the skin repair process using a murine model of cutaneous wound healing. We explored potential differences in wound closure and histological morphology between diet groups, and analyzed the expression and production of factors that participate in different stages of the repair process. Dietary supplementation with fish sidestream-derived collagen alone (Collagen), or in combination with a protein hydrolysate derived from salmon heads (HSH), resulted in accelerated healing. Chemical analysis of the tested extracts revealed that Collagen had the highest protein content and that HSH contained the great amount of zinc, known to support immune responses. Indeed, tissues from mice fed with collagen-containing supplements exhibited an increase in the expression levels of chemokines, important for the recruitment of immune cells into the damaged wound region. Moreover, expression of a potent angiogenic factor, vascular endothelial growth factor-A (VEGF-A), was elevated followed by enhanced collagen deposition. Our findings suggest that a 5%-supplemented diet with marine collagen-enriched supplements promotes tissue repair in the model of cutaneous wound healing, proposing a novel health-promoting use of fish sidestreams.

scholarly journals Accurate Region-of-Interest Recovery Improves the Measurement of the Cell Migration Rate in the In Vitro Wound Healing Assay

2017 ◽  
Vol 22 (6) ◽  
pp. 626-635
Author(s):  
Cesar Bedoya ◽  
Andrés Cardona ◽  
July Galeano ◽  
Fabián Cortés-Mancera ◽  
Patrick Sandoz ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Monolayer ◽  
Region Of Interest ◽  
Wound Healing Assay ◽  
Time Intervals ◽  
Positioning Errors ◽  
Cellular Events ◽  
Confluent Cell ◽  
A Cell

The wound healing assay is widely used for the quantitative analysis of highly regulated cellular events. In this essay, a wound is voluntarily produced on a confluent cell monolayer, and then the rate of wound reduction (WR) is characterized by processing images of the same regions of interest (ROIs) recorded at different time intervals. In this method, sharp-image ROI recovery is indispensable to compensate for displacements of the cell cultures due either to the exploration of multiple sites of the same culture or to transfers from the microscope stage to a cell incubator. ROI recovery is usually done manually and, despite a low-magnification microscope objective is generally used (10x), repositioning imperfections constitute a major source of errors detrimental to the WR measurement accuracy. We address this ROI recovery issue by using pseudoperiodic patterns fixed onto the cell culture dishes, allowing the easy localization of ROIs and the accurate quantification of positioning errors. The method is applied to a tumor-derived cell line, and the WR rates are measured by means of two different image processing software. Sharp ROI recovery based on the proposed method is found to improve significantly the accuracy of the WR measurement and the positioning under the microscope.

scholarly journals Wnt/β-catenin Signaling Pathway Promotes Transdifferentiation from Fetal Skin Fibroblasts to Keratinocyte-like Cells

2021 ◽  
Author(s):  
Hongqing Zhao ◽  
Xinzhu Liu ◽  
Jiachen Sun ◽  
Yuezeng Niu ◽  
Kun Zhang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Signaling Pathway ◽  
Wound Repair ◽  
Culture Conditions ◽  
Skin Fibroblasts ◽  
Fetal Skin ◽  
Protein Levels ◽  
Conventional Culture ◽  
Cellular Events ◽  
Proliferation And Differentiation

Abstract Background: Wound healing is a dynamic, sequential,and complex physiological process, including a variety of cellular events, such as proliferation, adhesion, chemotaxis, and apoptosis. Skin fibroblasts and keratinocytes are the two most important cells involved in wound repair, and Relying on the proliferation and differentiation of keratinocytes to form epithelium to completely cover the wound is the most ideal result for wound repair, so expanding the source of keratinocytes is a huge challenge. In this study, we examined the phenomenon that fetal skin fibroblasts spontaneously transdifferentiated into keratinocyte-like cells in conventional culture, and evaluated the characteristics of KLCs and the potential mechanisms of the transdifferentiation process.Methods: HFF-1 were routinely cultured in ordinary DMEM medium for more than 40 days,and observed the cell morphology. The cytological properties of KLCs at the cellular and molecular levels were detected by RT-PCR, Western-blot, immunofluorescence, Transwell, and cell scratch experiments.The functionality and safety of KLCs were determined through wound healing and tumorigenicity experiments. And high-throughput transcriptome sequencing (RNA-seq) was performed to explore the mechanism underlying HFF-1 transdifferentiation.Results: The transdifferentiation process started on the 25th day and was completed by the 40th day. KLCs and KCs had similar expressions at the molecular and protein levels, both functioned similarly in wound healing and were non-tumorigenic.RNA-seq revealed that the transdifferentiation process was regulated by the activation of the classical Wnt/β-catenin signaling pathway, which could shorten the process to 10 days.Conclusion: This study demonstrates that HFF-1 can spontaneously transdifferentiate into KLCs with conventional culture conditions, and the Wnt/β-catenin signaling pathway regulates the transdifferentiation process.

scholarly journals Cellular and Molecular Characteristics of Scarless versus Fibrotic Wound Healing

2010 ◽  
Vol 2010 ◽  
pp. 1-11 ◽  
Author(s):  
Latha Satish ◽  
Sandeep Kathju
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Molecular Mechanisms ◽  
Skin Wound ◽  
Molecular Characteristics ◽  
Skin Wound Healing ◽  
Cellular Events ◽  
Compare And Contrast ◽  
Adult Skin ◽  
Fetal Wound

The purpose of this paper is to compare and contrast the discrete biology differentiating fetal wound repair from its adult counterpart. Integumentary wound healing in mammalian fetuses is essentially different from wound healing in adult skin. Adult (postnatal) skin wound healing is a complex and well-orchestrated process spurred by attendant inflammation that leads to wound closure with scar formation. In contrast, fetal wound repair occurs with minimal inflammation, faster re-epithelialization, and without the accumulation of scar. Although research into scarless healing began decades ago, the critical molecular mechanisms driving the process of regenerative fetal healing remain uncertain. Understanding the molecular and cellular events during regenerative healing may provide clues that one day enable us to modulate adult wound healing and consequently reduce scarring.

scholarly journals Wound healing: cellular mechanisms and pathological outcomes

Open Biology ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 200223 ◽  
Author(s):  
Holly N. Wilkinson ◽  
Matthew J. Hardman
Keyword(s):  
Wound Healing ◽  
Tissue Repair ◽  
Wound Repair ◽  
Complex Dynamic ◽  
Cellular Mechanisms ◽  
Cellular Events ◽  
High Prevalence ◽  
Healing Wounds ◽  
Socioeconomic Burden ◽  
Healing Impairment

Wound healing is a complex, dynamic process supported by a myriad of cellular events that must be tightly coordinated to efficiently repair damaged tissue. Derangement in wound-linked cellular behaviours, as occurs with diabetes and ageing, can lead to healing impairment and the formation of chronic, non-healing wounds. These wounds are a significant socioeconomic burden due to their high prevalence and recurrence. Thus, there is an urgent requirement for the improved biological and clinical understanding of the mechanisms that underpin wound repair. Here, we review the cellular basis of tissue repair and discuss how current and emerging understanding of wound pathology could inform future development of efficacious wound therapies.

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