Hypoxia pretreatment of bone marrow-derived mesenchymal stem cells seeded in a collagen-chitosan sponge scaffold promotes skin wound healing in diabetic rats with hindlimb ischemia

2015 ◽  
Vol 24 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Chuan Tong ◽  
Haojie Hao ◽  
Lei Xia ◽  
Jiejie Liu ◽  
Dongdong Ti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Hindlimb Ischemia ◽  
Skin Wound Healing

Bone marrow-derived mesenchymal stem cells and extracellular vesicles enriched collagen chitosan scaffold in skin wound healing (a rat model)

2020 ◽  
pp. 088532822096392
Author(s):  
Salma Abolgheit ◽  
Sally Abdelkader ◽  
Moustafa Aboushelib ◽  
Enas Omar ◽  
Radwa Mehanna
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Tensile Strength ◽  
Mesenchymal Stem Cells ◽  
Extracellular Vesicles ◽  
Skin Wound ◽  
Collagen Deposition ◽  
Skin Wound Healing ◽  
Chitosan Scaffolds

Background Over the past ten years, regenerative medicine has focused on the regeneration and the reconstruction of damaged, diseased, or lost tissues and organs. Skin, being the largest organ in the human body, had attained a good attraction in this field. Delayed wound healing is one of the most challenging clinical medicine complications. This study aimed to evaluate the collagen chitosan scaffold’s effect alone, or enriched with either bone marrow-derived mesenchymal stem cells (BM-MSCs) or their secreted extracellular vesicles (EVs) on the duration and quality of skin wound healing. Methods A full-thickness skin wound was induced on the back of 32 adult male Sprague-Dawley rats. The wounds were either covered with collagen chitosan scaffolds alone, scaffolds enriched with stem cells, or extracellular vesicles. Unprotected wounds were used as control. Healing duration, collagen deposition and alignment, CD 68+ macrophage count, and functional tensile strength of healed skin were assessed (α = 0.05, n = 8). Results The rate of skin healing was significantly accelerated in all treated groups compared to the control. Immuno-histochemical assessment of CD68+ macrophages showed enhanced macrophages count, in addition to higher collagen deposition and better collagen alignment in EVs and BM-MSCs treated groups compared to the control group. Higher tensile strength values reflected the better collagen deposition and alignment for these groups. EVs showed higher amounts of collagen deposition and better alignment compared to MSCs treated group. Conclusion The collagen chitosan scaffolds enriched with MSCs or their EVs improved wound healing and improved the quantity and remodeling of collagen with a better assignment to EVs.

Bone marrow-derived mesenchymal stem cells laden novel thermo-sensitive hydrogel for the management of severe skin wound healing

2018 ◽  
Vol 90 ◽  
pp. 159-167 ◽  
Author(s):  
Zhang Lei ◽  
Gurankit Singh ◽  
Zhang Min ◽  
Chen Shixuan ◽  
Kaige Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Skin Wound ◽  
Skin Wound Healing

scholarly journals Applications of Bone Marrow Mesenchymal Stem Cell and Bioscaffolds in Skin Wound Healing

2021 ◽  
Vol 7 (1) ◽  
pp. 29-37
Author(s):  
Farzaneh Chehelcheraghi ◽  
Keyword(s):  
Quality Of Life ◽  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Therapeutic Strategies ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Marrow Mesenchymal Stem Cells

Introduction: Skin wound healing is a multi-step process. It involves coordinated interactions between growth factors, matrix, microenvironment around the wound, and various cells. Patients' quality of life in chronic wounds is affected because, in addition to sequential treatments, they incur significant medical costs. This review study aims to summarize the evidence and report current knowledge about tissue engineering, skin wound healing, and therapeutic strategies using bone marrow mesenchymal stem cells were performed. Methods: Thus, much effort has been focused on developing novel therapeutic approaches for wound treatment. Stem cell-based therapeutic strategies have been proposed to treat these wounds. They have shown significant potential for improving the speed and quality of wound healing and skin regeneration. A set of published data on the use of mesenchymal stem cells and a variety of biological scaffolds in wound healing is presented. Besides, we discussed different perspectives. Conclusion: We concluded that by activating bone marrow mesenchymal stem cells on a biological scaffold, the condition of the wound healing process can be improved. Keywords: Humans, Mesenchymal Stem Cells, Quality of Life, Tissue Engineering, Wound Healing, Skin, Stem Cells.

scholarly journals Mesenchymal stem cells in skin wound healing

2016 ◽  
Vol 10 (3) ◽  
pp. 228-234 ◽  
Author(s):  
Işıl Aydemir ◽  
Şamil Öztürk ◽  
Pınar Kılıçaslan Sönmez ◽  
Mehmet İbrahim Tuğlu
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Skin Wound ◽  
Skin Wound Healing

scholarly journals Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jin Liu ◽  
Xinyu Qiu ◽  
Yajie Lv ◽  
Chenxi Zheng ◽  
Yan Dong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Mouse Skin ◽  
Skin Wound ◽  
Therapeutic Effects ◽  
Cutaneous Wound Healing ◽  
Apoptotic Bodies ◽  
Skin Wound Healing ◽  
Cutaneous Wound

Abstract Background As the major interface between the body and the external environment, the skin is liable to various injuries. Skin injuries often lead to severe disability, and the exploration of promising therapeutic strategies is of great importance. Exogenous mesenchymal stem cell (MSC)-based therapy is a potential strategy due to the apparent therapeutic effects, while the underlying mechanism is still elusive. Interestingly, we observed the extensive apoptosis of exogenous bone marrow mesenchymal stem cells (BMMSCs) in a short time after transplantation in mouse skin wound healing models. Considering the roles of extracellular vesicles (EVs) in intercellular communication, we hypothesized that the numerous apoptotic bodies (ABs) released during apoptosis may partially contribute to the therapeutic effects. Methods ABs derived from MSCs were extracted, characterized, and applied in mouse skin wound healing models, and the therapeutic effects were evaluated. Then, the target cells of ABs were explored, and the effects of ABs on macrophages were investigated in vitro. Results We found ABs derived from MSCs promoted cutaneous wound healing via triggering the polarization of macrophages towards M2 phenotype. In addition, the functional converted macrophages further enhanced the migration and proliferation abilities of fibroblasts, which together facilitated the wound healing process. Conclusions Collectively, our study demonstrated that transplanted MSCs promoted cutaneous wound healing partially through releasing apoptotic bodies which could convert the macrophages towards an anti-inflammatory phenotype that plays a crucial role in the tissue repair process.

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