scholarly journals Potential of a coevolved rust fungus for the management of Himalayan balsam in the British Isles: first field releases

Weed Research ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 37-49 ◽  
Author(s):  
C A Ellison ◽  
K M Pollard ◽  
S Varia
Keyword(s):  
Rust Fungus ◽  
British Isles ◽  
Himalayan Balsam

scholarly journals Impatiens glandulifera (Himalayan balsam) chloroplast genome sequence as a promising target for populations studies

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8739 ◽  
Author(s):  
Giovanni Cafa ◽  
Riccardo Baroncelli ◽  
Carol A. Ellison ◽  
Daisuke Kurose
Keyword(s):  
Rust Fungus ◽  
Molecular Study ◽  
Plant Evolution ◽  
Native Range ◽  
Impatiens Glandulifera ◽  
Promising Target ◽  
Himalayan Balsam ◽  
Chloroplast Genome Sequence ◽  
Genome Skimming ◽  
Low Coverage

Background Himalayan balsam Impatiens glandulifera Royle (Balsaminaceae) is a highly invasive annual species native of the Himalayas. Biocontrol of the plant using the rust fungus Puccinia komarovii var. glanduliferae is currently being implemented, but issues have arisen with matching UK weed genotypes with compatible strains of the pathogen. To support successful biocontrol, a better understanding of the host weed population, including potential sources of introductions, of Himalayan balsam is required. Methods In this molecular study, two new complete chloroplast (cp) genomes of I. glandulifera were obtained with low coverage whole genome sequencing (genome skimming). A 125-year-old herbarium specimen (HB92) collected from the native range was sequenced and assembled and compared with a 2-year-old specimen from UK field plants (HB10). Results The complete cp genomes were double-stranded molecules of 152,260 bp (HB92) and 152,203 bp (HB10) in length and showed 97 variable sites: 27 intragenic and 70 intergenic. The two genomes were aligned and mapped with two closely related genomes used as references. Genome skimming generates complete organellar genomes with limited technical and financial efforts and produces large datasets compared to multi-locus sequence typing. This study demonstrates the suitability of genome skimming for generating complete cp genomes of historic herbarium material. It also shows that complete cp genomes are solid genetic markers for population studies that could be linked to plant evolution and aid with targeting native range and natural enemy surveys for biocontrol of invasive species.

High-pressure freezing and freeze substitution of teliospores of the rust fungus Gymnosporangium clavipes

1990 ◽  
Vol 48 (3) ◽  
pp. 692-693
Author(s):  
Robert W. Roberson
Keyword(s):  
High Pressure ◽  
Room Temperature ◽  
Pathogenic Fungus ◽  
Rust Fungus ◽  
Freeze Substitution ◽  
Ice Crystals ◽  
High Pressure Freezing ◽  
Thin Walled Structures ◽  
Cytological Changes ◽  
Dextran Solution

The use of cryo-techniques for the preparation of biological specimens in electron microscopy has led to superior preservation of ultrastructural detail. Although these techniques have obvious advantages, a critical limitation is that only 10-40 μm thick cells and tissue layers can be frozen without the formation of distorting ice crystals. However, thicker samples (600 μm) may be frozen well by rapid freezing under high-pressure (2,100 bar). To date, most work using cryo-techniques on fungi have been confined to examining small, thin-walled structures. High-pressure freezing and freeze substitution are used here to analysis pre-germination stages of specialized, sexual spores (teliospores) of the plant pathogenic fungus Gymnosporangium clavipes C & P.Dormant teliospores were incubated in drops of water at room temperature (25°C) to break dormancy and stimulate germination. Spores were collected at approximately 30 min intervals after hydration so that early cytological changes associated with spore germination could be monitored. Prior to high-pressure freezing, the samples were incubated for 5-10 min in a 20% dextran solution for added cryoprotection during freezing. Forty to 50 spores were placed in specimen cups and holders and immediately frozen at high pressure using the Balzers HPM 010 apparatus.

Illustrations and the genesis of Barrett and Yonge's Collins pocket guide to the sea shore (1958)

2010 ◽  
Vol 37 (2) ◽  
pp. 274-291 ◽  
Author(s):  
P. G. Moore
Keyword(s):  
Crucial Role ◽  
British Isles ◽  
Publishing History ◽  
Biological Station ◽  
Marine Biological ◽  
Illustrated Text ◽  
History Of ◽  
The University

Twenty nine items of correspondence from the mid-1950s discovered recently in the archives of the University Marine Biological Station Millport, and others made available by one of the illustrators and a referee, shed unique light on the publishing history of Collins pocket guide to the sea shore. This handbook, generally regarded as a classic of its genre, marked a huge step forwards in 1958; providing generations of students with an authoritative, concise, affordable, well illustrated text with which to identify common organisms found between the tidemarks from around the coasts of the British Isles. The crucial role played by a select band of illustrators in making this publication the success it eventually became, is highlighted herein. The difficulties of accomplishing this production within commercial strictures, and generally as a sideline to the main employment of the participants, are revealed. Such stresses were not helped by changing demands on the illustrators made by the authors and by the publishers.

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