In vitro invasiveness and intracellular survival of Salmonella strains isolated from the aquatic environment

2018 ◽  
Vol 33 (4) ◽  
pp. 633-640 ◽  
Author(s):  
Karina Ramírez ◽  
Areli Burgueño‐Roman ◽  
Nohelia Castro‐del Campo ◽  
Héctor S. López‐Moreno ◽  
Cristobal Chaidez
Keyword(s):  
Aquatic Environment ◽  
Intracellular Survival ◽  
In Vitro Invasiveness

B16 melanoma secretomes and in vitro invasiveness: syntenin as an invasion modulator

2010 ◽  
Vol 20 (2) ◽  
pp. 77-84 ◽  
Author(s):  
Fabien Rondepierre ◽  
Bernadette Bouchon ◽  
Mathilde Bonnet ◽  
Nicole Moins ◽  
Jean M. Chezal ◽  
...  
Keyword(s):  
B16 Melanoma ◽  
In Vitro Invasiveness

scholarly journals Studies on the metabolism and degradation of vancomycin in simulated in vitro and aquatic environment by UHPLC-Triple-TOF-MS/MS

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Mengsi Cao ◽  
Yanru Feng ◽  
Yan Zhang ◽  
Weijun Kang ◽  
Kaoqi Lian ◽  
...  
Keyword(s):  
Aquatic Environment ◽  
Tof Ms

Rv2223c, an acid inducible carboxyl-esterase of Mycobacterium tuberculosis enhanced the growth and survival of Mycobacterium smegmatis

2019 ◽  
Vol 14 (16) ◽  
pp. 1397-1415
Author(s):  
Pratibha Maan ◽  
Jagdeep Kaur
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Smegmatis ◽  
Intracellular Survival ◽  
Growth And Survival ◽  
Enhanced Expression ◽  
Stress Environment ◽  
In Vitro Stress ◽  
Carboxyl Esterase

Aim: To elucidate the role of Rv2223c in Mycobacterium tuberculosis. Methods: Purified recombinant Rv2223c protein was characterized. Expression of rv2223c in the presence of different stress environment and subcellular localization were performed in M. tuberculosis H37Ra and Mycobacterium smegmatis ( MS_2223c). Effect of its overexpression on growth rate, infection and intracellular survival in THP-1/PBMC cells were studied. Results: rRv2223c demonstrated esterase activity with preference for pNP-octanoate and hydrolyzed trioctanoate to di- and mono-octanoate. Expression of rv2223c was upregulated in acidic and nutritive stress conditions. rRv2223c was identified in extracellular and cell wall fractions. MS_2223c exhibited enhanced growth, survival during in vitro stress, infection and intracellular survival. Conclusions: Rv2223c is a secretary, carboxyl-esterase, with enhanced expression under acidic and nutritive stress condition and might help in intracellular survival of bacteria.

scholarly journals hLH/hCG-receptor expression correlates with in vitro invasiveness in human primary endometrial cancer

2008 ◽  
Vol 111 (3) ◽  
pp. 496-501 ◽  
Author(s):  
Ivo Noci ◽  
Serena Pillozzi ◽  
Elena Lastraioli ◽  
Sara Dabizzi ◽  
Massimo Giachi ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Receptor Expression ◽  
In Vitro Invasiveness ◽  
Hcg Receptor

Antisense Tiam1 Down-Regulates the Invasiveness of 95D Cells in Vitro

2004 ◽  
Vol 36 (8) ◽  
pp. 537-540 ◽  
Author(s):  
Min Hou ◽  
Li Tan ◽  
Xia Wang ◽  
Yun-Song Zhu
Keyword(s):  
Giant Cell ◽  
Lung Carcinoma ◽  
Expression Level ◽  
Nucleotide Exchange ◽  
Invasion And Metastasis ◽  
Cell Lung Carcinoma ◽  
Lung Carcinoma Cells ◽  
T Lymphoma ◽  
In Vitro Invasiveness

Abstract As a specific guanine nucleotide exchange factor of Rac1, Tiam1 (T-lymphoma invasion and metastasis inducing protein 1) is involved in a number of cellular events, such as cytoskeleton reorganization, cell adhesion, and cell migration. Since Tiam1 was implicated in the invasion and metastasis of T-lymphoma cells and breast tumor cells, we compared the expression level of Tiam1 in two human giant-cell lung carcinoma cell strains with high or low metastasis potential, and found that Tiam1 expression level in high-metastatic 95D cells was higher than that in low-metastatic 95C cells. To further confirm the role of Tiam1 in invasion and metastasis, we constructed the antisense Tiam1 expression plasmid (pcDNA3-anti-Tiam1), which was transfected into 95D cells. A stable transfected clone with decreased Tiam1 expression was screened and selected for further research. Transwell assay showed that down-regulation of endogenous Tiam1 by anti-Tiam1 can reduce the in vitro invasiveness of 95D cells. Our results suggested that Tiam1 signaling contributed to the invasion and metastasis of the human giant-cell lung carcinoma cells.

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