Open‐source image analysis software yields reproducible MRI measures of lumbar intervertebral disc degeneration in sheep models

2021 ◽  
Author(s):  
Jeryl Jones ◽  
Cerano Harrison ◽  
Alison Harbold ◽  
William Bridges ◽  
Jeremy Mercuri
Keyword(s):  
Image Analysis ◽  
Intervertebral Disc ◽  
Open Source ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Source Image ◽  
Analysis Software ◽  
Lumbar Intervertebral Disc ◽  
Image Analysis Software

scholarly journals ChromaWizard: An open source image analysis software for multicolor fluorescence in situ hybridization analysis

2018 ◽  
Vol 93 (7) ◽  
pp. 749-754
Author(s):  
Norbert Auer ◽  
Astrid Hrdina ◽  
Chaitra Hiremath ◽  
Sabine Vcelar ◽  
Martina Baumann ◽  
...  
Keyword(s):  
Image Analysis ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Open Source ◽  
Hybridization Analysis ◽  
Source Image ◽  
Analysis Software ◽  
Image Analysis Software ◽  
Multicolor Fluorescence

Development of CD3 cell quantitation algorithms for renal allograft biopsy rejection assessment utilizing open source image analysis software

2017 ◽  
Vol 472 (2) ◽  
pp. 259-269 ◽  
Author(s):  
Andres Moon ◽  
Geoffrey H. Smith ◽  
Jun Kong ◽  
Thomas E. Rogers ◽  
Carla L. Ellis ◽  
...  
Keyword(s):  
Image Analysis ◽  
Open Source ◽  
Renal Allograft ◽  
Source Image ◽  
Analysis Software ◽  
Image Analysis Software ◽  
Allograft Biopsy

scholarly journals Effect of the adenovirus-mediated Wip1 gene on lumbar intervertebral disc degeneration in a rabbit model

2017 ◽  
Vol 16 (6) ◽  
pp. 9487-9493 ◽  
Author(s):  
Yuan Wang ◽  
Yong Yang ◽  
Jing-Chuan Sun ◽  
Qin-Jie Kong ◽  
Hai-Bo Wang ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Rabbit Model ◽  
Lumbar Intervertebral Disc

Association between Measures of Vertebral Endplate Morphology and Lumbar Intervertebral Disc Degeneration

2017 ◽  
Vol 68 (2) ◽  
pp. 210-216 ◽  
Author(s):  
Semra Duran ◽  
Mehtap Cavusoglu ◽  
Hatice Gul Hatipoglu ◽  
Deniz Sozmen Cılız ◽  
Bulent Sakman
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Lumbar Disc ◽  
Lumbar Disc Degeneration ◽  
Vertebral Endplate ◽  
Lumbar Intervertebral Disc ◽  
Sagittal Diameter ◽  
Magnetic Resonance Imaging Mri ◽  
Vertebral Endplates

Purpose The aim of this study was to evaluate the association between vertebral endplate morphology and the degree of lumbar intervertebral disc degeneration via magnetic resonance imaging (MRI). Methods In total, 150 patients who met the inclusion criteria and were 20–60 years of age were retrospectively evaluated. Patients were evaluated for the presence of intervertebral disc degeneration or herniation, and the degree of degeneration was assessed at all lumbar levels. Vertebral endplate morphology was evaluated based on the endplate sagittal diameter, endplate sagittal concave angle (ECA), and endplate sagittal concave depth (ECD) on sagittal MRI. The association between intervertebral disc degeneration or herniation and endplate morphological measurements was analysed. Results In MRI, superior endplates ( ie, inferior endplates of the superior vertebra) were concave and inferior endplates ( ie, superior endplates of the inferior vertebra) were flat at all disc levels. A decrease in ECD and an increase in ECA were detected at all lumbar levels as disc degeneration increased ( P < .05). At the L4-L5 and L5-S1 levels, a decrease in ECD and an increase in ECA were detected in the group with herniated lumbar discs ( P < .05). There was no association between lumbar disc degeneration or herniation and endplate sagittal diameter at lumbar intervertebral levels ( P > .05). At all levels, ECD of women was significantly lesser than that of men and ECA of women was significantly greater than that of men ( P < .05). Conclusions There is an association between vertebral endplate morphology and lumbar intervertebral disc degeneration. Vertebral endplates at the degenerated disc level become flat; the severity of this flattening is correlated with the degree of disc degeneration.

scholarly journals Expression of ANGPTL4 in Nucleus Pulposus Tissues Is Associated with Intervertebral Disc Degeneration

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Fan-jie Liu ◽  
Liang-yu Xie ◽  
Hua-zhong Li ◽  
Sheng-nan Cao ◽  
Yuan-zhen Chen ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Western Blot ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Intervertebral Disc Herniation ◽  
Lumbar Intervertebral Disc ◽  
Real Time Quantitative Pcr ◽  
Lumbar Intervertebral Disc Herniation ◽  
Mrna And Protein Expression

Objective. Angiopoietin-like protein 4 (ANGPTL4), encoding a glycosylated secreted protein, has been reported to be closely related to many kinds of diseases, including diabetes, tumor, and some musculoskeletal pathologies, such as rheumatoid arthritis, osteoarthritis, and osteoporosis. The aim of the current study is to investigate the role of ANGPTL4 in intervertebral disc degeneration and analyze the association of ANGPTL4 expression with Pfirrmann grades. Methods. A total of 162 nucleus pulposus tissues were collected from lumbar intervertebral disc herniation patients undergoing interforaminal endoscopic surgery. Real-time quantitative PCR and western blot were performed to determine the mRNA and protein expression of ANGPTL4 in nucleus pulposus samples. Statistical analysis was performed to analyze the association of ANGPTL4 expression with Pfirrmann grades. Results. Based on the clinical data of 162 patients, results showed that Pfirrmann grades were significantly associated with patients’ age ( r = 0.162 , P = 0.047 ) and were not significantly associated with patients’ gender ( P > 0.05 ). RT-qPCR and western blot results showed that the mRNA ( r = 0.287 , P < 0.05 ) and protein ( r = 0.356 , P < 0.05 ) expressions of ANGPTL4 were both closely associated with Pfirrmann grades. The expression of ANGPTL4 was remarkably increased in the groups of high IVDD Pfirrmann grades. Conclusion. The results demonstrated that ANGPTL4 expression was positively associated with the Pfirrmann grades and the severity of intervertebral disc degeneration. ANGPTL4 may be served as a candidate biomarker for intervertebral disc degeneration.

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