Next-generation sequencing: academic overkill or high-resolution routine blood group genotyping?

2015 ◽  
Vol 10 (S1) ◽  
pp. 250-256 ◽  
Author(s):  
N. D. Avent ◽  
T. E. Madgett ◽  
A. J. Halawani ◽  
M. A. Altayar ◽  
M. Kiernan ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
High Resolution ◽  
Blood Group ◽  
Next Generation ◽  
Routine Blood ◽  
Generation Sequencing

scholarly journals Microsatellite marker development for the tetraploid Veronica aragonensis (Plantaginaceae) using next-generation sequencing and high-resolution melting analyses

2018 ◽  
Vol 6 (5) ◽  
pp. e01154
Author(s):  
Nélida Padilla-García ◽  
Teresa Malvar-Ferreras ◽  
Josie Lambourdière ◽  
M. Montserrat Martínez-Ortega ◽  
Nathalie Machon
Keyword(s):  
Next Generation Sequencing ◽  
High Resolution ◽  
Microsatellite Marker ◽  
High Resolution Melting ◽  
Marker Development ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals Next-generation sequencing is a credible strategy for blood group genotyping

2014 ◽  
Vol 167 (4) ◽  
pp. 554-562 ◽  
Author(s):  
Yann Fichou ◽  
Marie-Pierre Audrézet ◽  
Paul Guéguen ◽  
Cédric Le Maréchal ◽  
Claude Férec
Keyword(s):  
Next Generation Sequencing ◽  
Blood Group ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals Next-Generation Sequencing Technologies in Blood Group Typing

2019 ◽  
Vol 47 (1) ◽  
pp. 4-13 ◽  
Author(s):  
Daniel Fürst ◽  
Chrysanthi Tsamadou ◽  
Christine Neuchel ◽  
Hubert Schrezenmeier ◽  
Joannis Mytilineos ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Blood Group ◽  
Large Scale ◽  
Cost Effective ◽  
Molecular Testing ◽  
Blood Group Antigens ◽  
Next Generation ◽  
Sequencing Technologies ◽  
Blood Group Typing ◽  
Generation Sequencing

Sequencing of the human genome has led to the definition of the genes for most of the relevant blood group systems, and the polymorphisms responsible for most of the clinically relevant blood group antigens are characterized. Molecular blood group typing is used in situations where erythrocytes are not available or where serological testing was inconclusive or not possible due to the lack of antisera. Also, molecular testing may be more cost-effective in certain situations. Molecular typing approaches are mostly based on either PCR with specific primers, DNA hybridization, or DNA sequencing. Particularly the transition of sequencing techniques from Sanger-based sequencing to next-generation sequencing (NGS) technologies has led to exciting new possibilities in blood group genotyping. We describe briefly the currently available NGS platforms and their specifications, depict the genetic background of blood group polymorphisms, and discuss applications for NGS approaches in immunohematology. As an example, we delineate a protocol for large-scale donor blood group screening established and in use at our institution. Furthermore, we discuss technical challenges and limitations as well as the prospect for future developments, including long-read sequencing technologies.

scholarly journals High-resolution, high-throughput HLA genotyping by next-generation sequencing

2009 ◽  
Vol 74 (5) ◽  
pp. 393-403 ◽  
Author(s):  
G. Bentley ◽  
R. Higuchi ◽  
B. Hoglund ◽  
D. Goodridge ◽  
D. Sayer ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
High Resolution ◽  
High Throughput ◽  
Next Generation ◽  
Hla Genotyping ◽  
Generation Sequencing

190-P: High-resolution KIR genotyping on pooled samples by next generation sequencing

2009 ◽  
Vol 70 ◽  
pp. S107
Author(s):  
Martha B. Ladner ◽  
Gordon Bentley ◽  
Damian Goodridge ◽  
Henry A. Erlich ◽  
Elizabeth Trachtenberg
Keyword(s):  
Next Generation Sequencing ◽  
High Resolution ◽  
Next Generation ◽  
Pooled Samples ◽  
Generation Sequencing

scholarly journals High resolution clustering of Salmonella enterica serovar Montevideo strains using a next-generation sequencing approach

BMC Genomics ◽  
2012 ◽  
Vol 13 (1) ◽  
pp. 32 ◽  
Author(s):  
Marc W Allard ◽  
Yan Luo ◽  
Errol Strain ◽  
Cong Li ◽  
Christine E Keys ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
High Resolution ◽  
Salmonella Enterica ◽  
Next Generation ◽  
Generation Sequencing
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