Automated point-of-care testing for ABO agglutination test: proof of concept and validation

Vox Sanguinis ◽  
2015 ◽  
Vol 109 (1) ◽  
pp. 79-85 ◽  
Author(s):  
H. El Kenz ◽  
F. Corazza
Keyword(s):  
Point Of Care ◽  
Agglutination Test ◽  
Proof Of Concept ◽  
Point Of Care Testing

scholarly journals Switching from Multiplex to Multimodal Colorimetric Lateral Flow Immunosensor

Sensors ◽  
2020 ◽  
Vol 20 (22) ◽  
pp. 6609
Author(s):  
Simone Cavalera ◽  
Fabio Di Nardo ◽  
Luca Forte ◽  
Francesca Marinoni ◽  
Matteo Chiarello ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Proof Of Concept ◽  
Point Of Care Testing ◽  
Human Immunoglobulins ◽  
Immunodeficiency Virus ◽  
Specific Antigens ◽  
Increasing Demand ◽  
Gold Nanomaterials ◽  
Two Parameter

Multiplex lateral flow immunoassay (LFIA) is largely used for point-of-care testing to detect different pathogens or biomarkers in a single device. The increasing demand for multitargeting diagnostics requires multi-informative single tests. In this study, we demonstrated three strategies to upgrade standard multiplex LFIA to multimodal capacity. As a proof-of-concept, we applied the strategies to the differential diagnosis of Human Immunodeficiency Virus (HIV) infection, a widespread pathogen, for which conventional multiplex LFIA testing is well-established. In the new two-parameter LFIA (x2LFIA), we exploited color encoding, in which the binding of multiple targets occurs in one reactive band and the color of the probe reveals which one is present in the sample. By combining the sequential alignment of several reactive zones along the membrane of the LFIA strip and gold nanoparticles and gold nanostars for the differential visualization, in this demonstration, the x2LFIA can furnish information on HIV serotype and stage of infection in a single device. Three immunosensors were designed. The use of bioreagents as the capturing ligand anchored onto the membrane or as the detection ligand labelled with gold nanomaterials affected the performance of the x2LFIA. Higher detectability was achieved by the format involving the HIV-specific antigens as capturing agent and labelled secondary bioligands (anti-human immunoglobulins M and protein G) as the probes.

scholarly journals Point-of-care testing to promote cardiovascular disease risk assessment: A proof of concept study

2017 ◽  
Vol 7 ◽  
pp. 136-139 ◽  
Author(s):  
Kunal N. Karmali ◽  
Tiffany Brown ◽  
Thomas Sanchez ◽  
Timothy Long ◽  
Stephen D. Persell
Keyword(s):  
Cardiovascular Disease ◽  
Risk Assessment ◽  
Disease Risk ◽  
Point Of Care ◽  
Cardiovascular Disease Risk ◽  
Proof Of Concept ◽  
Point Of Care Testing ◽  
Concept Study

scholarly journals A Compact, Syringe-Assisted, Vacuum-Driven Micropumping Device

Micromachines ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 543 ◽  
Author(s):  
Anyang Wang ◽  
Domin Koh ◽  
Philip Schneider ◽  
Evan Breloff ◽  
Kwang W. Oh
Keyword(s):  
Microfluidic Device ◽  
Point Of Care ◽  
Microfluidic Devices ◽  
Microfluidic Channels ◽  
Constant Flow ◽  
Proof Of Concept ◽  
Point Of Care Testing ◽  
Microfluidic Mixing ◽  
Pumping System ◽  
Dead End

In this paper, a simple syringe‑assisted pumping method is introduced. The proposed fluidic micropumping system can be used instead of a conventional pumping system which tends to be large, bulky, and expensive. The micropump was designed separately from the microfluidic channels and directly bonded to the outlet of the microfluidic device. The pump components were composed of a dead‑end channel which was surrounded by a microchamber. A syringe was then connected to the pump structure by a short tube, and the syringe plunger was manually pulled out to generate low pressure inside the microchamber. Once the sample was loaded in the inlet, air inside the channel diffused into the microchamber through the PDMS (polydimethylsiloxane) wall, acting as a dragging force and pulling the sample toward the outlet. A constant flow with a rate that ranged from 0.8 nl · s − 1 to 7.5 nl · s − 1 was achieved as a function of the geometry of the pump, i.e., the PDMS wall thickness and the diffusion area. As a proof-of-concept, microfluidic mixing was demonstrated without backflow. This method enables pumping for point-of-care testing (POCT) with greater flexibility in hand-held PDMS microfluidic devices.

Point-of-care testing by paramedics using a portable laboratory: an evaluation

2020 ◽  
Vol 12 (3) ◽  
pp. 100-108
Author(s):  
Katy Heaney ◽  
Kerry Whiting ◽  
Lynda Petley ◽  
Ian Fry ◽  
Andy Newton
Keyword(s):  
Point Of Care ◽  
Direct Consequence ◽  
Data Capture ◽  
Added Value ◽  
Electronic Data Capture ◽  
Proof Of Concept ◽  
Point Of Care Testing ◽  
Patient Interactions ◽  
Effective Use ◽  
Support Decision Making

Use of point-of-care testing (POCT) equipment by paramedics for triage may reduce unnecessary attendance in emergency departments and inconvenience to patients. A hospital pathology service and an ambulance trust wanted a system for safe and effective use of diagnostic devices by paramedics at the patient bedside. A suite of POCT devices to perform an expanded repertoire of pathology tests was provided, along with technology for electronic data capture, temperature control and monitoring, in a specially designed kit bag—the Labkit. Following a proof-of-concept phase, three Labkit bags were deployed as a pilot in rapid response vehicles and used by specialist paramedics in urgent and emergency care who had been trained in their use. The paramedics used the bag in 25% of patient interactions, typically three times every 24 hours. Having POCT results available at the time of paramedic assessment reduced conveyance to the emergency department by 21%. There was also a 10% rise in admission of patients where pathology results indicated problems that required urgent treatment which would otherwise have gone unnoticed. Overall, 31% of conveyance decisions were changed as a direct consequence of the Labkit results. Patients reported high levels of satisfaction, and paramedics said it added value in 97% of cases where it was used to support decision-making. Reliable, quality-assured POCT by paramedics has the potential to improve efficiency in the healthcare system and benefit patients.

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