scholarly journals Automation of blood component preparation from whole blood collections

Vox Sanguinis ◽  
2014 ◽  
Vol 107 (1) ◽  
pp. 10-18 ◽  
Author(s):  
J. Cid ◽  
L. Magnano ◽  
M. Lozano
Keyword(s):  
Whole Blood ◽  
Blood Component

scholarly journals 1792. Viral DNA Loads in Various Blood Components of Patients with EBV-Positive T/NK Cell Lymphoproliferative Diseases

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S660-S661
Author(s):  
Jun-ichi Kawada ◽  
Yasuko Kamiya ◽  
Akihisa Sawada ◽  
Keiji Iwatsuki ◽  
Koji Izustu ◽  
...  
Keyword(s):  
Disease Activity ◽  
Infectious Mononucleosis ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Nk Cell ◽  
Inactive Disease ◽  
Blood Component ◽  
Blood Components ◽  
Ebv Dna

Abstract Background Epstein–Barr virus (EBV) is associated with T- and NK-cell lymphoproliferative disorders (EBV T/NK-LPD). For diagnosis of EBV T/NK-LPD, quantification of EBV DNA loads in peripheral blood by real-time PCR has been widely used. However, optimal blood components and cut-off values for diagnosis were not fully evaluated. Methods Fifty-nine patients with EBV T/NK-LPD including chronic active EBV infection (CAEBV), severe mosquito bite allergy, hydroa vacciniforme-like lymphoproliferative disorder (HV), and EBV- hemophagocytic lymphohistiocytosis (EBV-HLH) were enrolled. EBV DNA loads were compared among disease categories in each blood component from the same whole blood sample. The association between EBV DNA loads and disease activity were evaluated in CAEBV patients. Furthermore, the diagnostic cut-off value for EBV DNA loads in whole blood from CAEBV patients as compared with infectious mononucleosis patients was determined. Results EBV DNA loads in whole blood and peripheral blood mononuclear cells (PBMCs) were not significantly different among disease categories, whereas EBV DNA loads in plasma were significantly higher in EBV- HLH patients than in HV patients. EBV DNA loads in whole blood and PBMCs showed strong correlation (Figure 1). EBV DNA loads in plasma were significantly higher in CAEBV patients with active disease than in those with inactive disease (median: 104.5 IU/mL vs. 100.8 IU/mL, P < 0.001) (Figure 2). Diagnostic cut-off values for whole blood EBV DNA loads of CAEBV patients as compared with those of infectious mononucleosis was 104.2 ( = 15,800) IU/mL (Figure 3). Conclusion Measuring EBV DNA loads in whole blood can be considered as initial evaluation for diagnosis of EBV T/NK-LPD. EBV DNA loads in plasma are more closely related to disease activity of CAEBV than EBV DNA loads in whole blood and PBMCs. Disclosures All authors: No reported disclosures.

New Options for Multicomponent Collection (MCC) with the Latest Cell Separators

1998 ◽  
Vol 21 (6_suppl) ◽  
pp. 26-30 ◽  
Author(s):  
R. Bruni ◽  
M. Valbonesi ◽  
R. Frisoni ◽  
G. Lercari ◽  
M.R. Ruzzenenti ◽  
...  
Keyword(s):  
Health Care ◽  
Whole Blood ◽  
Total Content ◽  
Blood Component ◽  
Red Cell ◽  
Preliminary Results ◽  
Original Configuration

Recently, several new cell separators have become available for high platelet low WBC contamination thrombocytapheresis. In their original configuration, these apparatuses can only collect plasma along with platelets, while no concurrent red cell collection can be carried out. In this study, we present the preliminary results of plasma and/or erythrothrombocytapheresis after the adaptation of the lines of two different cell separators, namely the Fresenius AS 204 and the Baxter Health Care Amicus. In addition, the preliminary results of multicomponent collection using the Dideco Excel will be presented; in the latter a system of plasma recirculation was introduced, to increase the purity and quality of the platelet product. The results of these studies show that erythrothrombocytapheresis can be carried out with all these apparatuses, but only with Dideco-Excel and the Fresenius AS 204 the procedure is totally automated. There are marginal differences in the quality of the RBC product in terms of percent hematocrit, from 65% with the Excel and AS 204 to ∼ 75% with the Amicus and in terms total content of hemoglobin which averages 58 g per RBC concentrate when RBC collection is carried out during the processing of ∼ 420 ml of whole blood. In terms of platelet collection, the differences are presently moderate, as the platelet yield and WBC contamination are not affected by the concurrent RBC collection. The average platelet yield was 3.98-4.1, and 4.01x1011 and the total WBC contamination was 1.2-2.9, and 1.9X105 rerspectively with the Amicus, the AS 204 and the Excel. These represent a new step in the direction of total apheresis for blood and blood component procurement.

Comparison of blood component preparation methods from whole blood bags based on buffy coat extraction

2007 ◽  
Vol 36 (3) ◽  
pp. 243-247 ◽  
Author(s):  
Joan Cid ◽  
Montse Claparols ◽  
Asunción Pinacho ◽  
José Manuel Hernández ◽  
Pilar Ortiz ◽  
...  
Keyword(s):  
Whole Blood ◽  
Buffy Coat ◽  
Blood Component ◽  
Preparation Methods

scholarly journals Pattern of blood component cross-matching and their utilization in a tertiary care hospital of Jammu region

2018 ◽  
Vol 6 (4) ◽  
pp. 1337 ◽  
Author(s):  
Irm Yasmeen ◽  
Ibrar Ahmed ◽  
Meena Sidhu
Keyword(s):  
Whole Blood ◽  
Tertiary Care ◽  
Blood Component ◽  
Care Hospital ◽  
Blood Components ◽  
Cross Sectional ◽  
Inappropriate Use ◽  
Wide Range ◽  
Surgical Conditions ◽  
Cross Matching

Background: Transfusion of donated blood remains the mainstay of treatment for a wide range of medical and surgical conditions. Although it can save life, but transfusion of blood is not without risk. Clinicians should cautiously assess the appropriateness of indications before requesting various blood components thereby preventing misuse of blood and unnecessary exposure of patient to various transfusion transmitted infections and antibodies production. This study was conducted to determine the pattern of whole blood (WB) and blood component cross-matching and their utilization and to minimize the inappropriate use of blood and its components.Methods: This cross-sectional prospective study was performed at SMGS Hospital Blood Bank, Jammu from April 2016 to September 2016. The requisition forms were analysed at the reception counter and inside the pre-transfusion testing laboratory for any error. The department wise utilization of blood and its components, Crossmatching to transfusion (C/T) ratio, transfusion probability (T%) and transfusion index (TI) were calculated.Results: A total of 14376 requests for cross-matching of blood and its components were received. All the units were cross-matched. Out of these, 12766(88.8%) units of blood and its components were issued to various departments. The most common indication for using packed red cells and whole blood was anemia and bleeding (APH/PPH/Trauma). The total C/T Ratio, transfusion probability (T%) and Transfusion index(TI)  of various blood components were 1.12:1, 88.8% and 0.88 respectively.Conclusions: Our study indicates efficient usage of blood and its component. However, awareness is still needed amongst the clinicians and residents to ensure the appropriate use of blood and its components in the future as well. Hospital transfusion committee has to develop transfusion guidelines and subsequent implementation of such guidelines to assure effective blood utilization. MSBOS (maximum surgical blood ordering schedule) should be formulated for elective procedures with regular auditing, feedback, and modifications to improve blood ordering and utilization.

scholarly journals Flow Properties of Modified Packed Red Cells

1987 ◽  
Vol 15 (4) ◽  
pp. 407-410 ◽  
Author(s):  
V. A. Lovric ◽  
M. Schuller ◽  
G. Rose ◽  
J. Raftos ◽  
M. Stewart ◽  
...  
Keyword(s):  
Blood Flow ◽  
Centrifugal Force ◽  
Whole Blood ◽  
Red Cells ◽  
Free Flow ◽  
Blood Component ◽  
Flow Properties ◽  
Blood Storage ◽  
Duration Of Storage

We investigated free gravitational blood flow of modified packed red cells. Variables affecting free flow included final haematocrit, duration of blood storage at 4–6°C and the centrifugal force required in preparation of this blood component. Irrespective of the duration of storage, free blood flow of modified packed red cells was not different from whole blood, provided the haematocrit did not exceed 0.63.

scholarly journals Blood Component Therapy

2018 ◽  
Vol 9 (2) ◽  
pp. 142-147
Author(s):  
Shanaz Karim ◽  
Ehteshamul Hoque ◽  
Md Mazharul Hoque ◽  
Syeda Masooma Rahman ◽  
Kashfia Islam
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Fresh Frozen Plasma ◽  
Blood Component ◽  
Packed Red Blood Cells ◽  
Medical College ◽  
Blood Component Therapy ◽  
Fresh Frozen ◽  
Cellular Components

Transfusion medicine has undergone advancements since its initiation in the early 20th century. One of these was the discovery that blood can be divided into individual components and delivered separately. Today, blood transfusions nearly always consist of the ad-ministration of 1 or more components of blood. Whole blood transfusion is now limited to situations involving massive resuscitation (trauma ) The most familiar cellular components include packed red blood cells (PRBC), washed PRBC, leukoreduced PRBC and pooled or aphaeresis platelets. Plasma products such as FFP or cryoprecipitate, ant hemophilic factor (CRYO). The transfusion of red blood cells (RBCs), platelets, fresh-frozen plasma (FFP), and cryoprecipitate has the potential of improving clinical outcomes in perioperative and peripartum settings. These benefits include improved tissue oxygenation and decreased bleeding. However, transfusions are not without risks or costs. With the advent of blood component therapy, each unit of whole blood collected serves the specific needs of several, rather than a single patient.Anwer Khan Modern Medical College Journal Vol. 9, No. 2: Jul 2018, P 142-147

Prestorage Leukocyte Depletion with In-Line Filtration of Whole Blood in Comparison with Blood Component Leukocyte Depletion

Vox Sanguinis ◽  
1995 ◽  
Vol 69 (3) ◽  
pp. 201-205
Author(s):  
J. Riggert ◽  
G. Simson ◽  
J. Dittmann ◽  
M. Köhler
Keyword(s):  
Whole Blood ◽  
Blood Component ◽  
Leukocyte Depletion

scholarly journals Whole blood versus component therapy for haemostatic resuscitation of major bleeding: a protocol for a systematic review and meta-analysis

BMJ Open ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. e043967
Author(s):  
Anna E Ssentongo ◽  
Paddy Ssentongo ◽  
Emily Heilbrunn ◽  
Lacee Laufenberg Puopolo ◽  
Vernon M Chinchilli ◽  
...  
Keyword(s):  
Systematic Review ◽  
Major Bleeding ◽  
Whole Blood ◽  
Meta Analysis ◽  
Risk Of Bias ◽  
Primary Data ◽  
Blood Component ◽  
Geographical Differences ◽  
Blood Component Therapy ◽  
Haemostatic Resuscitation

IntroductionThere is a renewed interest in the use of whole blood (WB) to manage patients with life-threatening bleeding. We aimed to estimate mortality and complications risk between WB and blood component therapy for haemostatic resuscitation of major bleeding.MethodsWe will conduct a systematic review and meta-analysis of studies published between 1 January 1980 and 1 January 2020, identified from PubMed and Scopus databases. Population will be patients who require blood transfusion (traumatic operative, obstetric and gastrointestinal bleeding). Intervention is WB transfusion such as fresh WB (WB unit stored for less than 48 hours), leukoreduced modified WB (with platelets removed during filtration), warm fresh WB (stored warm at 22°C for up to 8 hours and then for a maximum of an additional 24 hours at 4°C). The primary outcomes will be the 24-hour and 30-day survival rates (in-hospital mortality). Comparator is blood component therapy (red blood cells, fresh-frozen plasma and platelets given together in a 1:1:1 unit ratio). The Cochrane risk of bias tool for randomised controlled trials and Risk Of Bias In Non-randomised Studies - of Interventions (ROBINS-I) for observation studies will be used to assess the risk of bias of included studies. We will use random-effects models for the pooling of studies. Interstudy heterogeneity will be assessed by the Cochran Q statistic, where p<0.10 will be considered statistically significant and quantified by I2 statistic, where I2 ≥50% will indicate substantial heterogeneity. We will perform subgroup and meta-regression analyses to assess geographical differences and other study-level factors explaining variations in the reported mortality risk. Results will be reported as risk ratios and their 95% CIs.Ethics and disseminationNo ethics clearance is required as no primary data will be collected. The results will be presented at scientific conferences and published in a peer-reviewed journal.

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