scholarly journals Surgical treatment of canine and feline descemetoceles, deep and perforated corneal ulcers with autologous buccal mucous membrane grafts

2021 ◽  
Author(s):  
Valentina Mezzadri ◽  
Alberto Crotti ◽  
Samanta Nardi ◽  
Giovanni Barsotti
Keyword(s):  
Surgical Treatment ◽  
Mucous Membrane ◽  
Corneal Ulcers ◽  
Buccal Mucous Membrane

Simplified Fixation and Staining for Delineation of Nuclear Chromatin by the Barr Technique

PEDIATRICS ◽  
1959 ◽  
Vol 24 (5) ◽  
pp. 858-859
Author(s):  
A. ROBINSON
Keyword(s):  
Mucous Membrane ◽  
Somatic Cells ◽  
Screening Procedure ◽  
Nuclear Chromatin ◽  
Clinical Tool ◽  
Chromosomal Constitution ◽  
Large Populations ◽  
Buccal Mucous Membrane ◽  
Original Procedure

Examination of smears of the buccal mucous membrane for nuclear chromatin has become a useful clinical tool in the evaluation of conditions involving questions of chromosomal sex. Although actual delineation of the complete chromosomal constitution of the patient's somatic cells yields more information than the nuclear chromatin determination alone. the latter test is extremely useful because of its simplicity. This makes it readily available for use on any patient and applicable to large populations as a screening procedure for detection of certain kinds of anomalies. Hence, it seems worthwhile to call attention to the following modification of the Barr test for nuclear chromatin, which is more rapid than the original procedure, but appears to be every bit as reliable.

The buccal mucous membrane

1911 ◽  
Vol 5 (9) ◽  
pp. 447-455 ◽  
Author(s):  
James Crawford Watt
Keyword(s):  
Mucous Membrane ◽  
Buccal Mucous Membrane

scholarly journals Clinical Aspects of Amniotic Membrane Implants in Corneal Repair in Dogs with Complicated Ulcers

2020 ◽  
Vol 48 ◽  
Author(s):  
Adriano Rozin ◽  
Karla Menezes Cardoso ◽  
Tarcísio Guerra Guimarães
Keyword(s):  
Surgical Treatment ◽  
Protease Inhibitors ◽  
Amniotic Membrane ◽  
Clinical Aspects ◽  
Eye Drops ◽  
Absorbable Suture ◽  
Time Points ◽  
Corneal Ulcers ◽  
Corneal Vascularization ◽  
Mediators Of Inflammation

Background: Among numerous disorders treated by veterinary ophthalmology, therapies employed to solve corneal ulcers stand out. Amniotic membrane transplantation is an effective surgical technique for the treatment of complicated corneal ulcers in the dog, with highly satisfactory visual and cosmetic outcomes. However, in veterinary ophthalmology, reports on the use of the amniotic membrane and its corneal reconstructive potential are scarce. The objective of this work was to evaluate, for 21 days, the clinical aspects and the effectiveness of transplantation of canine amniotic membrane for corneal healing in two cases of complicated ulcers in dogs.Cases: Two Shih-Tzu dogs were diagnosed with complicated corneal ulcer. They underwent surgical treatment with the use of a glycerin-preserved canine amniotic membrane implant, which was secured on the cornea or the limbus. A surgical microscope at a magnification of 16x was used. The devitalized tissues at the periphery of the ulcer were excised, and multiple layers of amniotic membranes were sutured onto the cornea and near the limbus with interrupted absorbable suture. Protection of the canine amniotic membrane implants was performed with a scarified third eyelid flap, which was attached to the upper eyelid, and maintained for 14 days. During the first 14 days after the procedure, topical treatment with antibiotic eye drops was administered; protease inhibitors were also used. Antibiotics were used systemically for 10 days. Between days 14 and 21 after the transplantation procedure, protease inhibitors, corticosteroids, and lubricant were administered topically. Healing and clinical aspects were evaluated on days 0, 14, and 21. The parameters evaluated were blepharospasm, dazzle and direct pupillary reflexes, and consensual and threat responses. Corneal vascularization, hypopyon, synechia, opacity, and fluorescein dye penetration into the cornea were evaluated. The patient whose membrane was attached to the cornea was named COr, and the animal whose membrane was attached to the corneal limbus was named PLi. Blepharospasm and positive fluorescein test were detected only on the first day of the experiment; whereas, the dazzle and pupillary reflexes, as well as the consensual response, were present in both animals at all evaluated time points. Only COr exhibited hypopyon on day 0. Synechia was not observed in any of the animals at any of the time points. On the 21st day, COr exhibited deep vascularization and an opaque scar, while PLi exhibited only discrete vessels without blood perfusion, secondary to corneal repair.Discussion: A smooth operation of the central and peripheral ophthalmic system was maintained at all periods evaluated. The protection provided by the membrane contributed to corneal healing by reducing stimulation of nerve endings on the epithelium and stroma, and suppressing the blepharospasm reflex. The amniotic membrane used as an adjuvant has antimicrobial and anti-inflammatory properties, inhibits proteinases and mediators of inflammation, and exerts a mechanically protective effect. The corneal vascularization observed in both animals is related to the healing processes stimulated by filling the deep corneal lesions with the membrane. When used in addition to drug therapy, the amniotic membrane can inhibit postinflammatory neovascularization, fibroblast activity, collagen deposition, scarring, and opacifications. Accurate diagnosis, and correct therapeutic and surgical management, is fundamental for the successful treatment of corneal ulcers. In this work, canine amniotic membrane preserved in glycerin was used for the surgical treatment of complicated ulcers in dogs along with adequate clinical therapy. This was effective independent of the different anchorage techniques used, and led to satisfactory clinical results and short-term corneal healing. 

Histological Examination in the Prognosis of Mixed Tumors of the Palate

1964 ◽  
Vol 50 (6) ◽  
pp. 457-468
Author(s):  
Federico Pizzetti ◽  
Roberto Molinari ◽  
Emilio Restelli
Keyword(s):  
Surgical Treatment ◽  
Parotid Gland ◽  
Histological Examination ◽  
Mucous Membrane ◽  
Mixed Tumor ◽  
Mixed Tumors ◽  
Better Than

The review of 21 cases of mixed tumor of the palate showed that the prognosis, after surgical treatment, is much better than in mixed tumors of the parotid gland. No recurrence was seen in 20 cases, although histologically they often showed great cellularity and pseudo-cylindromatous or muco-epidermoid areas. In the only case which evolved unfavourably, invasive tendency toward the mucous membrane and cylindromatous areas were found.

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