Altered mRNA and protein expression of filaggrin in the skin of a canine animal model for atopic dermatitis

2013 ◽  
Vol 24 (3) ◽  
pp. 329-e73 ◽  
Author(s):  
Domenico Santoro ◽  
Rosanna Marsella ◽  
Kim Ahrens ◽  
Thomas K. Graves ◽  
David Bunick
Keyword(s):  
Animal Model ◽  
Atopic Dermatitis ◽  
Protein Expression ◽  
Mrna And Protein Expression

AN ANIMAL MODEL OF PEYRONIE'S-LIKE CONDITION ASSOCIATED WITH AN INCREASE OF TRANSFORMING GROWTH FACTOR BETA mRNA AND PROTEIN EXPRESSION

1997 ◽  
Vol 158 (6) ◽  
pp. 2284-2290 ◽  
Author(s):  
Ahmed I. El-Sakka ◽  
Howayda M. Hassoba ◽  
Richard M. Chui ◽  
Rajendra S. Bhatnagar ◽  
Rajvir Dahiya ◽  
...  
Keyword(s):  
Animal Model ◽  
Growth Factor ◽  
Protein Expression ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Mrna And Protein Expression ◽  
Growth Factor Beta

Abstract A51: p53 and p21 mRNA and protein expression in treated synthetic estrogen in mouse transgenic animal model

2013 ◽  
Author(s):  
Mohd Nazil Salleh ◽  
Tan Mei Cheng ◽  
Thuaibah Hashim ◽  
Henkie Isahwan Ahamd Mulyadi Lai ◽  
Wan Khairuzzaman Wan Ramli
Keyword(s):  
Animal Model ◽  
Protein Expression ◽  
Transgenic Animal ◽  
Transgenic Animal Model ◽  
Synthetic Estrogen ◽  
Mrna And Protein Expression

scholarly journals Apigenin Inhibits IL-31 Cytokine in Human Mast Cell and Mouse Skin Tissues

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1290 ◽  
Author(s):  
Denis Nchang Che ◽  
Byoung Ok Cho ◽  
Jae Young Shin ◽  
Hyun Ju Kang ◽  
Ji-Su Kim ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Mast Cells ◽  
Protein Expression ◽  
Inflammatory Diseases ◽  
Mouse Skin ◽  
Human Mast Cell ◽  
Biological Applications ◽  
Potential Applicability ◽  
Mrna And Protein Expression

IL-31 is a recently discovered cytokine that is produced not only in T-cells but also in mast cells. It is strongly implicated to play a key role in inflammatory diseases and in the pathogenesis of itch in atopic dermatitis. Apigenin, a flavonoid of plant origin has numerous biological applications. In this study, we showed that apigenin modulates IL-31 mRNA, protein expression, and release in stimulated human mast (HMC-1) by inhibiting the phosphorylation activation of MAPK and NF-κB. To determine whether apigenin has similar effects in vivo, using Compound 48/80, we developed an atopic dermatitis itch model in mice and found an increase in IL-31 expression in the skin. We also revealed that apigenin prevents the infiltration and degranulation of mast cells and suppressed mRNA and protein expression of IL-31 in the skin of mice. These results provide a new suggestion of the potential applicability of apigenin for treatment of various inflammatory diseases and itch mediated by IL-31.

Bone sialoprotein mRNA and protein expression in human multiple myeloma cell lines and patients

2000 ◽  
Vol 111 (4) ◽  
pp. 1118-1121 ◽  
Author(s):  
A. Bellahcene ◽  
I. Van Riet ◽  
C. de Greef ◽  
N. Antoine ◽  
M. F. Young ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Protein Expression ◽  
Cell Lines ◽  
Myeloma Cell ◽  
Bone Sialoprotein ◽  
Multiple Myeloma Cell ◽  
Human Multiple Myeloma ◽  
Mrna And Protein Expression

Changes in mRNA and Protein Expression of Antioxidant Enzymes in Leukocytes of Children with Bronchial Asthma after Interval Hypoxic Training

2012 ◽  
Vol 3 (2) ◽  
pp. 125-134
Author(s):  
Klaudia V. Nesvitaylova ◽  
Olga A. Gonchar ◽  
Tatyana I. Drevitskaya ◽  
Ludmila P. Arabskaya ◽  
Mikhail M. Steshenko ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Protein Expression ◽  
Bronchial Asthma ◽  
Hypoxic Training ◽  
Mrna And Protein Expression

Icariin Stimulates hFOB 1.19 Osteoblast Proliferation and Differentiation via OPG/RANKL Mediated by the Estrogen Receptor

2020 ◽  
Vol 22 (1) ◽  
pp. 168-175 ◽  
Author(s):  
Lin-Jun Sun ◽  
Chong Li ◽  
Xiang-hao Wen ◽  
Lu Guo ◽  
Zi-Fen Guo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Estrogen Receptor ◽  
Protein Expression ◽  
Chinese Herb ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Expression Ratio ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Mrna And Protein Expression

Background:: Icariin (ICA), one of the main effective components isolated from the traditional Chinese herb Epimedium brevicornu Maxim., has been reported to possess extensive pharmacological actions, including enhanced sexual function, immune regulation, anti-inflammation, and antiosteoporosis. Methods:: Our study was designed to investigate the effect of ICA on cell proliferation and differentiation and the molecular mechanism of OPG/RANKL mediated by the Estrogen Receptor (ER) in hFOB1.19 human osteoblast cells. Results:: The experimental results show that ICA can stimulate cell proliferation and increase the activity of Alkaline Phosphatase (ALP), Osteocalcin (BGP) and I Collagen (Col I) and a number of calcified nodules. Furthermore, the mRNA and protein expression of OPG and RANKL and the OPG/ RANKL mRNA and protein expression ratios were upregulated by ICA. The above-mentioned results indicated that the optimal concentration of ICA for stimulating osteogenesis was 50ng/mL. Subsequent mechanistic studies comparing 50ng/mL ICA with an estrogen receptor antagonist demonstrated that the effect of the upregulated expression is connected with the estrogen receptor. In conclusion, ICA can regulate bone formation by promoting cell proliferation and differentiation and upregulating the OPG/RANKL expression ratio by the ER in hFOB1.19 human osteoblast cells.

Effects of Morphine on Interstitial Cells of Cajal in Rabbit Colon and Small Intestinal Transit: An Experimental Study

2020 ◽  
Vol 20 (3) ◽  
pp. 240-246
Author(s):  
Heng Yang ◽  
Xiao-Ju Jin ◽  
Hong Luo ◽  
Yuan-Hai Li
Keyword(s):  
Protein Expression ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Saline Control ◽  
Morphine Group ◽  
Small Intestinal Transit ◽  
Medium Concentration ◽  
Epidural Puncture ◽  
Mrna And Protein Expression ◽  
Cells Of Cajal

Objective: This study aims to investigate the effect of morphine with naloxone on intestinal peristalsis and the number of interstitial cells of Cajal (ICC) in colon tissues of rabbits. Methods: Thirty rabbits were randomly divided into five groups (n=6, each group): saline control group (NS group), low concentration of morphine group (L group), medium concentration of morphine group (M group), high concentration of morphine group (H group), medium concentration of morphine and naloxone mixed with antagonist group (NM group). Rabbits in these five groups were administered with an epidural puncture tube and dorsal epidural analgesia pump, and were continuously infused for seven days. Fecal characteristics were observed, and the ink propulsion rate was calculated. The expression level of ICC C-kit protein in colon tissues was tested by western blot. Results: The stool characteristics in the L, M and H groups were more severe than those in the NS and NM groups. Furthermore, the intestinal propulsion rate in the L, M and H groups was lower than that in the NS and NM groups. The C-kit mRNA and protein expression in the colon of rabbits were significantly lower in the L, M and H groups, when compared to the NS and NM groups. Conclusions: Naloxone blocked the mRNA and protein expression of C-kit, and improved intestinal motor function.

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