Lipoprotein characterization in Quaker parrots ( Myiopsitta monachus ) using gel‐permeation high‐performance liquid chromatography

2020 ◽  
Vol 49 (3) ◽  
pp. 417-427
Author(s):  
Hugues Beaufrère ◽  
Sara Gardhouse ◽  
Mélanie Ammersbach
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Myiopsitta Monachus ◽  
Gel Permeation

Analysis of 188Re-EDTMP complexes by HPLC and ultrafiltration

2004 ◽  
Vol 92 (4-6) ◽  
Author(s):  
Kazuyuki Hashimoto ◽  
Hiromitsu Matsuoka
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Anion Exchange ◽  
High Performance ◽  
Gel Permeation Chromatography ◽  
Reversed Phase ◽  
Ion Pair ◽  
Gel Permeation

AbstractHigh performance liquid chromatography (anion-exchange, reversed-phase ion-pair and gel permeation chromatography) and ultrafiltration have been employed to analyze

A novel pituitary protein (7B2)-like immunoreactivity is secreted by a rat phaeochromocytoma cell line (PC12)

1986 ◽  
Vol 108 (1) ◽  
pp. 151-155 ◽  
Author(s):  
H. Suzuki ◽  
A. S. Tischler ◽  
N. D. Christofides ◽  
M. Chretien ◽  
N. G. Seidah ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cell Line ◽  
Pc12 Cell ◽  
High Performance ◽  
Gel Permeation Chromatography ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Gel Permeation ◽  
High Concentrations

ABSTRACT High concentrations of a novel pituitary protein (7B2) have been shown to be present in the PC12 rat phaeochromocytoma cell line by radioimmunoassay. 7B2-like immunoreactivity (IR-7B2) was released from PC12 cells into the incubation medium in response to stimulation by a depolarizing concentration of K+, and this K+-evoked release was inhibited by Co2+, The major IR-7B2 in PC12 cell and medium appeared to be identical to that in porcine pituitary gland as judged by both gel permeation chromatography and by reverse-phase high performance liquid chromatography (HPLC). Gel permeation chromatography of extracts of cell and medium revealed two IR-7B2 peaks, the earlier eluting at a elution coefficient (Kav) of 0·30 and the later at a Kav of 0·54. In medium, over 90% of the IR-7B2 eluted as the earlier peak. Fractionation of extracts of cell and medium on reverse-phase HPLC showed three main IR-7B2 peaks eluting at 43, 44·5 and 46% acetonitrile/water with 0·1% trifluoroacetic acid. The findings suggest that IR-7B2 might be released by calcium-mediated exocytosis. J. Endocr. (1986) 108, 151–155

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