Serum protein concentration and protein fractions in clinically healthy Lacaune and Sarda sheep using agarose gel electrophoresis

2015 ◽  
Vol 44 (4) ◽  
pp. 564-569 ◽  
Author(s):  
Arianna Miglio ◽  
Maria Teresa Antognoni ◽  
Carmen Maresca ◽  
Claudia Moncada ◽  
Fulvio Riondato ◽  
...  
Keyword(s):  
Serum Protein ◽  
Gel Electrophoresis ◽  
Protein Concentration ◽  
Protein Fractions ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Serum Protein Concentration

Evaluation of usefulness of a commercial agarose gel electrophoresis kit (QuickGel SP) for bovine serum protein electrophoresis

2017 ◽  
Vol 20 (3) ◽  
pp. 527-534
Author(s):  
Y. Okatsu ◽  
N. Yamagishi ◽  
K. Hatate ◽  
B. Devkota
Keyword(s):  
Serum Protein ◽  
Gel Electrophoresis ◽  
Bovine Serum ◽  
Protein Fractions ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Serum Samples ◽  
Cellulose Acetate Electrophoresis ◽  
Γ Globulins ◽  
Bovine Serum Protein

Abstract The aim of this study was to show the usefulness of a commercial agarose gel electrophoresis (AGE) kit (QuickGel SP) for separating bovine serum protein fractions in comparison with conventional cellulose acetate electrophoresis (CAE). Serum protein bands were verified using five reference reagents corresponding to albumin and α1-, β1-, β2-, and γ-globulins. AGE clearly revealed six separated fractions of albumin and α1-, α2-, β1-, β2-, and γ-globulin fractions in 100% and 77.8% in serum samples of dairy cows from the healthy (n=27) and diseased groups (n=27), respectively. The α1- and α2-globulins were not separated by CAE in 14.8% and 96.3% of the samples from the healthy and diseased groups, respectively, whereas β2- and γ-globulin were not separated by CAE in 96.3% and 100% of the samples from the healthy and diseased groups, respectively. More than 94% of the points for the α-globulin fractions (α1- and α2-globulins), the β-γ-globulin fractions (β1-, β2-, and γ-globulins), and the albumin/globulin ratio between AGE and CAE were within agreement on the Bland-Altman plots. However, the mean biases were not near zero in the albumin and β-γ-globulin fractions. These results suggest that the high-resolution commercial AGE kit can be utilized to separate bovine serum protein fractions.

Comparative study on serum protein concentration and electrophoretic pattern in clinically healthy gazelles

2019 ◽  
Author(s):  
A. M. Gar-Elnabi ◽  
I. A. Alhidary ◽  
A. O. Bakhiet ◽  
K. A. Abdoun
Keyword(s):  
Serum Protein ◽  
Gel Electrophoresis ◽  
Protein Concentration ◽  
Electrophoretic Pattern ◽  
Normal Serum ◽  
Agarose Gel Electrophoresis ◽  
Protein Electrophoretic Pattern ◽  
Clinical Conditions ◽  
G Ratio ◽  
Serum Protein Concentration

Screening of electrophoretic pattern of serum protein is crucial for the initial evaluation of numerous clinical conditions. The present study aimed to investigate and compare the normal serum protein electrophoretic pattern in three gazelle species which included sand gazelle (Gazella gazella), mountain gazelle (Gazella subgutterosa) and Erlanger’s gazelle (Gazella erlangeri). Significant (P £ 0.05) interspecies differences were noticed in A/G ratio, and in á-1, â and globulins fractions using agarose gel electrophoresis. Significant (P £ 0.05) differences between males of the three species were encountered in á-1, á- 2 and â globulins fractions, while significant differences in A/G ratio, á-1, á-2 and â globulins fractions were observed between females of the three species. Significance (P £ 0.05) intersex differences were observed for albumin in sand gazelle, and for á-2 and â globulins fractions in mountain gazelle. The results obtained from the current study could provide a baseline references and could be used for diagnostic purposes.

Serum protein concentrations and protein fractions in clinically healthy Italian Heavy Draft Horses using agarose gel electrophoresis

2019 ◽  
Vol 48 (4) ◽  
pp. 677-682 ◽  
Author(s):  
Arianna Miglio ◽  
Chiara Morelli ◽  
Carmen Maresca ◽  
Andrea Felici ◽  
Andrea Di Gianbattista ◽  
...  
Keyword(s):  
Serum Protein ◽  
Gel Electrophoresis ◽  
Protein Fractions ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis

Absence of beta-globulin band in the serum protein electropherogram of a patient with liver disease.

1981 ◽  
Vol 27 (2) ◽  
pp. 334-336 ◽  
Author(s):  
A O Vladutiu ◽  
J S Kim
Keyword(s):  
Liver Cirrhosis ◽  
Liver Disease ◽  
Serum Protein ◽  
Gel Electrophoresis ◽  
Marked Decrease ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Glycoprotein I ◽  
Complement Component C3 ◽  
Beta Globulin

Abstract Agarose-gel electrophoresis of serum of a 72-year-old woman with liver cirrhosis showed virtually no beta-globulins two weeks before the patient's death. There was marked decrease in the concentrations of transferrin, beta-lipoproteins, hemopexin, complement component C3, beta-glycoprotein I, and cholesterol in serum. Absence of a beta-globulin band appears to signify an ominous prognosis.

Serum protein concentrations from clinically healthy horses determined by agarose gel electrophoresis

2009 ◽  
Vol 38 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Barbara Riond ◽  
Bettina Wenger-Riggenbach ◽  
Regina Hofmann-Lehmann ◽  
Hans Lutz
Keyword(s):  
Serum Protein ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis
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