Therapeutic potential of the cytosine deaminase::uracil phosphoribosyl transferase/5‐fluorocytosine suicide system for canine melanoma

2021 ◽  
Author(s):  
Gerardo C. Glikin ◽  
Jesica B. Allende ◽  
Liliana M. E. Finocchiaro
Keyword(s):  
Therapeutic Potential ◽  
Cytosine Deaminase ◽  
Phosphoribosyl Transferase ◽  
Canine Melanoma

scholarly journals Therapeutic potential of bleomycin plus suicide or interferon-β gene transfer combination for spontaneous feline and canine melanoma

Oncoscience ◽  
2017 ◽  
Vol 4 (11-12) ◽  
pp. 199-214 ◽  
Author(s):  
Lucrecia Agnetti ◽  
Chiara Fondello ◽  
Marcela S. Villaverde ◽  
Gerardo C. Glikin ◽  
Liliana M.E. Finocchiaro
Keyword(s):  
Gene Transfer ◽  
Therapeutic Potential ◽  
Interferon Β ◽  
Canine Melanoma

scholarly journals Tissue‐specific inactivation by cytosine deaminase/uracil phosphoribosyl transferase as a tool to study plant biology

2019 ◽  
Vol 101 (3) ◽  
pp. 731-741
Author(s):  
Nathalie Leonhardt ◽  
Fanchon Divol ◽  
Serge Chiarenza ◽  
Sabrina Deschamps ◽  
Jeanne Renaud ◽  
...  
Keyword(s):  
Cytosine Deaminase ◽  
Plant Biology ◽  
Tissue Specific ◽  
Phosphoribosyl Transferase

Combination of cytosine deaminase with uracil phosphoribosyl transferase leads to local and distant bystander effects against RM1 prostate cancer in mice

2006 ◽  
Vol 8 (9) ◽  
pp. 1086-1096 ◽  
Author(s):  
Aparajita Khatri ◽  
Bing Zhang ◽  
Eboney Doherty ◽  
Jane Chapman ◽  
Kim Ow ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cytosine Deaminase ◽  
Bystander Effects ◽  
Phosphoribosyl Transferase

Bystander effect from cytosine deaminase and uracil phosphoribosyl transferase genes in vitro: A partial contribution of gap junctions

2009 ◽  
Vol 282 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Toshiaki Tanaka ◽  
Agnès Duflot-Dancer ◽  
Michèle Tiraby ◽  
Colette Piccoli ◽  
Gérard Tiraby ◽  
...  
Keyword(s):  
Gap Junctions ◽  
Bystander Effect ◽  
Cytosine Deaminase ◽  
Partial Contribution ◽  
Phosphoribosyl Transferase

scholarly journals Precise and programmable C:G to G:C base editing in genomic DNA

2020 ◽  
Author(s):  
Liwei Chen ◽  
Jung Eun Park ◽  
Peter Paa ◽  
Priscilla D. Rajakumar ◽  
Yi Ting Chew ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Excision Repair ◽  
Genetic Diseases ◽  
Cytosine Deaminase ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Base Editing ◽  
Guide Rnas ◽  
Base Excision ◽  
Nucleotide Resolution

AbstractMany genetic diseases are caused by single-nucleotide polymorphisms (SNPs). Base editors can correct SNPs at single-nucleotide resolution, but until recently, only allowed for C:G to T:A and A:T to G:C transition edits, addressing four out of twelve possible DNA base substitutions. Here we developed a novel class of C:G to G:C Base Editors (CGBEs) to create single-base genomic transversions in human cells. Our CGBEs consist of a nickase CRISPR-Cas9 (nCas9) fused to a cytosine deaminase and base excision repair (BER) proteins. Characterization of >30 CGBE candidates and 27 guide RNAs (gRNAs) revealed that CGBEs predominantly perform C:G to G:C editing (up to 90% purity), with rAPOBEC-nCas9-rXRCC1 being the most efficient (mean C:G to G:C edits at 15% and up to 37%). CGBEs target cytosine in WCW, ACC or GCT sequence contexts and within a precise two-nucleotide window of the target protospacer. We further targeted genes linked to dyslipidemia, hypertrophic cardiomyopathy, and deafness, showing the therapeutic potential of CGBE in interrogating and correcting human genetic diseases.

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