Desmopressin in canine mammary carcinoma: Comments on the importance of the administration route

2020 ◽  
Author(s):  
Daniel F. Alonso ◽  
Esteban Turic ◽  
Juan Garona
Keyword(s):  
Mammary Carcinoma ◽  
Administration Route ◽  
Canine Mammary Carcinoma

Involvement of Nestin in the Progression of Canine Mammary Carcinoma

2021 ◽  
pp. 030098582110186
Author(s):  
Hisashi Yoshimura ◽  
Maiko Moriya ◽  
Ayaka Yoshida ◽  
Masami Yamamoto ◽  
Yukino Machida ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mammary Carcinoma ◽  
Mammary Tumors ◽  
Carcinoma Cells ◽  
Nestin Expression ◽  
Mammary Carcinomas ◽  
Canine Mammary Carcinoma ◽  
Canine Mammary Tumors ◽  
Mammary Carcinoma Cells ◽  
Luminal Epithelial Cells

Nestin, a class VI intermediate filament protein, is known to be expressed in various types of human neoplasms, including breast cancer, and is associated with their progression. However, its expression and role in canine mammary tumors remain unknown. We analyzed nestin expression in canine mammary tumors using in situ hybridization and immunohistochemistry. We also investigated its role in a canine mammary carcinoma cell line using RNA interference. Nestin expression was not observed in luminal epithelial cells of any of the 62 cases of benign mammary lesions examined, although myoepithelial cells showed its expression in most cases. In 16/50 (32%) primary mammary carcinomas and 6/15 (40%) metastases of mammary carcinomas, cytoplasmic nestin expression was detected in luminal epithelial cells. In luminal cells of primary mammary carcinomas, its expression was positively related to several pathological parameters that indicate high-grade malignancy, including histological grading ( P < .01), vascular/lymphatic invasion ( P < .01), Ki-67 index ( P < .01), and metastasis ( P < .05). Immunohistochemistry revealed that nestin expression was related to vimentin expression in mammary carcinomas ( P < .01). This relationship was confirmed using reverse transcription-quantitative polymerase chain reaction using 9 cell lines derived from canine mammary carcinoma ( P < .01). Finally, nestin knockdown in canine mammary carcinoma cells using small interfering RNA inhibited cell proliferation and migration based on WST-8, Boyden chamber, and cell-tracking assays. These findings suggest that nestin may at least partially mediate these behaviors of canine mammary carcinoma cells.

scholarly journals Rare type of grade ii lipid-rich canine mammary carcinoma

2020 ◽  
Vol 8 (4) ◽  
pp. 1937-1938
Author(s):  
M Thangapandiyan ◽  
V Kumar ◽  
P Krishnaveni ◽  
GV Sudhakar Rao
Keyword(s):  
Mammary Carcinoma ◽  
Rare Type ◽  
Canine Mammary Carcinoma ◽  
Grade Ii

TNF‐Related Apoptosis‐Inducing Ligand ( TRAIL ) Loss in Canine Mammary Carcinoma

2021 ◽  
Author(s):  
Soo‐Hyeon Kim ◽  
Byung‐Joon Seung ◽  
Min‐Kyung Bae ◽  
Ha‐Young Lim ◽  
Seung‐Hee Cho ◽  
...  
Keyword(s):  
Mammary Carcinoma ◽  
Canine Mammary Carcinoma
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