scholarly journals Extracellular cyclic adenosine monophosphate‐dependent protein kinase A autoantibody and C‐reactive protein as serum biomarkers for diagnosis of cancer in dogs

2018 ◽  
Vol 17 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Min‐Ok Ryu ◽  
Byung‐Gak Kim ◽  
Ul‐Soo Choi ◽  
Kwan‐Hyuck Baek ◽  
Young‐Ki Song ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Serum Biomarkers ◽  
C Reactive Protein ◽  
Dependent Protein Kinase ◽  
Reactive Protein ◽  
Diagnosis Of Cancer ◽  
Dependent Protein ◽  
Kinase A

scholarly journals The 3-phosphoinositide–dependent protein kinase 1 is an essential upstream activator of protein kinase A in malaria parasites

PLoS Biology ◽  
2021 ◽  
Vol 19 (12) ◽  
pp. e3001483
Author(s):  
Eva Hitz ◽  
Natalie Wiedemar ◽  
Armin Passecker ◽  
Beatriz A. S. Graça ◽  
Christian Scheurer ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Blood Stage ◽  
Targeted Mutagenesis ◽  
Missense Mutations ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Kinase A

Cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) signalling is essential for the proliferation of Plasmodium falciparum malaria blood stage parasites. The mechanisms regulating the activity of the catalytic subunit PfPKAc, however, are only partially understood, and PfPKAc function has not been investigated in gametocytes, the sexual blood stage forms that are essential for malaria transmission. By studying a conditional PfPKAc knockdown (cKD) mutant, we confirm the essential role for PfPKAc in erythrocyte invasion by merozoites and show that PfPKAc is involved in regulating gametocyte deformability. We furthermore demonstrate that overexpression of PfPKAc is lethal and kills parasites at the early phase of schizogony. Strikingly, whole genome sequencing (WGS) of parasite mutants selected to tolerate increased PfPKAc expression levels identified missense mutations exclusively in the gene encoding the parasite orthologue of 3-phosphoinositide–dependent protein kinase-1 (PfPDK1). Using targeted mutagenesis, we demonstrate that PfPDK1 is required to activate PfPKAc and that T189 in the PfPKAc activation loop is the crucial target residue in this process. In summary, our results corroborate the importance of tight regulation of PfPKA signalling for parasite survival and imply that PfPDK1 acts as a crucial upstream regulator in this pathway and potential new drug target.

8-pCPT, an Epac activator, impairs conditioned place preference based on nucleus accumbens amphetamine in rats

2013 ◽  
Vol 26 (2) ◽  
pp. 104-111 ◽  
Author(s):  
Sung Woo Park ◽  
Ali Roohbakhsh ◽  
Richard J. Beninger
Keyword(s):  
Nucleus Accumbens ◽  
Protein Kinase ◽  
Dopamine Receptor ◽  
Conditioned Place Preference ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Place Preference ◽  
Dependent Protein Kinase ◽  
Cyclic Monophosphate ◽  
Dependent Protein

ObjectivesDopamine receptor-mediated 3′,5′-cyclic adenosine monophosphate (cAMP)-dependent intracellular signalling is important for reward-related learning. cAMP activates cAMP-dependent protein kinase (PKA) and exchange protein directly activated by cAMP (Epac). We tested the hypothesis that reward-related learning may be mediated by Epac.MethodsWe evaluated conditioned place preference (CPP) on the basis of nucleus accumbens (NAc) injections of amphetamine (20 μg/0.5 μl/side) plus Sp-adenosine 3′,5′-cyclic monophosphorothioate triethylamanine (Sp-cAMPS) (0.1, 1.0, 10, 15, 20 μg/0.5 μl/side), an activator of both PKA and Epac, or amphetamine (20 μg) plus 8-(4-chlorophenylthio)-2′-O-methyladenosine-3′,5′-cyclic monophosphate (8-pCPT) (0.73, 1.27, 1.45, 2.89, 5.78, 11.56 μg/0.5 μl/side), an activator of Epac.ResultsIn agreement with previous results, Sp-cAMPS dose-dependently impaired CPP. 8-pCPT impaired CPP at one dose (1.45 μg/0.5 μl/side) and we replicated this effect three times.ConclusionThe results implicate Epac in the acquisition of reward-related learning.

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