Defect inND2,COX2,ATP6andCOX3mitochondrial genes as a risk factor for canine mammary tumour

2016 ◽  
Vol 15 (3) ◽  
pp. 1062-1072 ◽  
Author(s):  
M. Surdyka ◽  
B. Slaska
Keyword(s):  
Risk Factor ◽  
Mammary Tumour ◽  
Canine Mammary Tumour

scholarly journals Doxorubicin treatment modulates chemoresistance and affects the cell cycle in two canine mammary tumour cell lines

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Michela Levi ◽  
Roberta Salaroli ◽  
Federico Parenti ◽  
Raffaella De Maria ◽  
Augusta Zannoni ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Cell Lines ◽  
Tumour Cell ◽  
S Phase ◽  
Mammary Tumour ◽  
Cancer Resistance ◽  
Neoplastic Cells ◽  
Canine Mammary Tumour ◽  
Tumour Cell Lines

Abstract Background Doxorubicin (DOX) is widely used in both human and veterinary oncology although the onset of multidrug resistance (MDR) in neoplastic cells often leads to chemotherapy failure. Better understanding of the cellular mechanisms that circumvent chemotherapy efficacy is paramount. The aim of this study was to investigate the response of two canine mammary tumour cell lines, CIPp from a primary tumour and CIPm, from its lymph node metastasis, to exposure to EC50(20h) DOX at 12, 24 and 48 h of treatment. We assessed the uptake and subcellular distribution of DOX, the expression and function of P-glycoprotein (P-gp) and Breast Cancer Resistance Protein (BCRP), two important MDR mediators. To better understand this phenomenon the effects of DOX on the cell cycle and Ki67 cell proliferation index and the expression of p53 and telomerase reverse transcriptase (TERT) were also evaluated by immunocytochemistry (ICC). Results Both cell lines were able to uptake DOX within the nucleus at 3 h treatment while at 48 h DOX was absent from the intracellular compartment (assessed by fluorescence microscope) in all the surviving cells. CIPm, originated from the metastatic tumour, were more efficient in extruding P-gp substrates. By ICC and qRT-PCR an overall increase in both P-gp and BCRP were observed at 48 h of EC50(20h) DOX treatment in both cell lines and were associated with a striking increase in the percentage of p53 and TERT expressing cells by ICC. The cell proliferation fraction was decreased at 48 h in both cell lines and cell cycle analysis showed a DOX-induced arrest in the S phase for CIPp, while CIPm had an increase in cellular death without arrest. Both cells lines were therefore composed by a fraction of cells sensible to DOX that underwent apoptosis/necrosis. Conclusions DOX administration results in interlinked modifications in the cellular population including a substantial effect on the cell cycle, in particular arrest in the S phase for CIPp and the selection of a subpopulation of neoplastic cells bearing MDR phenotype characterized by P-gp and BCRP expression, TERT activation, p53 accumulation and decrease in the proliferating fraction. Important information is given for understanding the dynamic and mechanisms of the onset of drug resistance in a neoplastic cell population.

Canine Mammary Tumour with Smooth and Striated Muscle, Osseous and Chondroid Differentiation: Unusual Mixed Type or Mesenchymoma?

2017 ◽  
Vol 156 (1) ◽  
pp. 122
Author(s):  
A. Brunetti ◽  
G. Beha ◽  
L.V. Muscatello ◽  
L. De Tolla ◽  
G. Avallone
Keyword(s):  
Mixed Type ◽  
Striated Muscle ◽  
Mammary Tumour ◽  
Canine Mammary Tumour

Direct effect of a gonadotropin-releasing hormone agonist on the growth of canine mammary tumour cells

2002 ◽  
Vol 85 (3) ◽  
pp. 470-481 ◽  
Author(s):  
Ugo Pagnini ◽  
Salvatore Florio ◽  
Luca Crispino ◽  
Giuseppe Pagnini ◽  
Donato Colangelo ◽  
...  
Keyword(s):  
Direct Effect ◽  
Gonadotropin Releasing Hormone ◽  
Tumour Cells ◽  
Mammary Tumour ◽  
Releasing Hormone ◽  
Canine Mammary Tumour ◽  
Gonadotropin Releasing Hormone Agonist

Molecular cloning and characterisation of the tissue inhibitor of metalloproteinase-3 gene from canine mammary tumour

2013 ◽  
Vol 41 (3) ◽  
pp. 294-299
Author(s):  
B.V. Sunil Kumar ◽  
Pavan Kumar Yadav ◽  
K. Aswani Kumar ◽  
Barkha Ratta ◽  
Meeta Saxena ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Mammary Tumour ◽  
Tissue Inhibitor ◽  
Canine Mammary Tumour

Immortalization and characterization of a new canine mammary tumour cell line FR37-CMT

2016 ◽  
Vol 15 (3) ◽  
pp. 952-967 ◽  
Author(s):  
L. R. Raposo ◽  
C. Roma-Rodrigues ◽  
P. Faísca ◽  
M. Alves ◽  
J. Henriques ◽  
...  
Keyword(s):  
Cell Line ◽  
Tumour Cell ◽  
Mammary Tumour ◽  
Tumour Cell Line ◽  
Canine Mammary Tumour
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