Characterization ofHOXgene expression in canine mammary tumour cell lines from spontaneous tumours

2013 ◽  
Vol 13 (3) ◽  
pp. 322-336 ◽  
Author(s):  
P. DeInnocentes ◽  
A. L. Perry ◽  
E. C. Graff ◽  
F. M. Lutful Kabir ◽  
R. Curtis Bird
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Mammary Tumour ◽  
Canine Mammary Tumour ◽  
Tumour Cell Lines ◽  
Spontaneous Tumours

scholarly journals Doxorubicin treatment modulates chemoresistance and affects the cell cycle in two canine mammary tumour cell lines

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Michela Levi ◽  
Roberta Salaroli ◽  
Federico Parenti ◽  
Raffaella De Maria ◽  
Augusta Zannoni ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Cell Lines ◽  
Tumour Cell ◽  
S Phase ◽  
Mammary Tumour ◽  
Cancer Resistance ◽  
Neoplastic Cells ◽  
Canine Mammary Tumour ◽  
Tumour Cell Lines

Abstract Background Doxorubicin (DOX) is widely used in both human and veterinary oncology although the onset of multidrug resistance (MDR) in neoplastic cells often leads to chemotherapy failure. Better understanding of the cellular mechanisms that circumvent chemotherapy efficacy is paramount. The aim of this study was to investigate the response of two canine mammary tumour cell lines, CIPp from a primary tumour and CIPm, from its lymph node metastasis, to exposure to EC50(20h) DOX at 12, 24 and 48 h of treatment. We assessed the uptake and subcellular distribution of DOX, the expression and function of P-glycoprotein (P-gp) and Breast Cancer Resistance Protein (BCRP), two important MDR mediators. To better understand this phenomenon the effects of DOX on the cell cycle and Ki67 cell proliferation index and the expression of p53 and telomerase reverse transcriptase (TERT) were also evaluated by immunocytochemistry (ICC). Results Both cell lines were able to uptake DOX within the nucleus at 3 h treatment while at 48 h DOX was absent from the intracellular compartment (assessed by fluorescence microscope) in all the surviving cells. CIPm, originated from the metastatic tumour, were more efficient in extruding P-gp substrates. By ICC and qRT-PCR an overall increase in both P-gp and BCRP were observed at 48 h of EC50(20h) DOX treatment in both cell lines and were associated with a striking increase in the percentage of p53 and TERT expressing cells by ICC. The cell proliferation fraction was decreased at 48 h in both cell lines and cell cycle analysis showed a DOX-induced arrest in the S phase for CIPp, while CIPm had an increase in cellular death without arrest. Both cells lines were therefore composed by a fraction of cells sensible to DOX that underwent apoptosis/necrosis. Conclusions DOX administration results in interlinked modifications in the cellular population including a substantial effect on the cell cycle, in particular arrest in the S phase for CIPp and the selection of a subpopulation of neoplastic cells bearing MDR phenotype characterized by P-gp and BCRP expression, TERT activation, p53 accumulation and decrease in the proliferating fraction. Important information is given for understanding the dynamic and mechanisms of the onset of drug resistance in a neoplastic cell population.

cDNA microarray profiles of canine mammary tumour cell lines reveal deregulated pathways pertaining to their phenotype

2008 ◽  
Vol 39 (4) ◽  
pp. 333-345 ◽  
Author(s):  
N. A. S. Rao ◽  
M. E. van Wolferen ◽  
R. van den Ham ◽  
D. van Leenen ◽  
M. J. A. Groot Koerkamp ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cdna Microarray ◽  
Tumour Cell ◽  
Mammary Tumour ◽  
Canine Mammary Tumour ◽  
Tumour Cell Lines

Immunophenotype of Mammary Tumour Cell Lines for In-Vitro Analysis Using Three Different Techniques

2020 ◽  
Vol 174 ◽  
pp. 184
Author(s):  
F. Torrigiani ◽  
A. Sammarco ◽  
M.E. Gelain ◽  
F. Bonsembiante ◽  
R. Zanetti ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Mammary Tumour ◽  
Tumour Cell Lines ◽  
Vitro Analysis ◽  
In Vitro Analysis

In-vitro anti-proliferative effects of some anti-tumour drugs on feline mammary tumour cell lines

1999 ◽  
Vol 66 (3) ◽  
pp. 169-174 ◽  
Author(s):  
J.S MULEYA ◽  
M NAKAICHI ◽  
Y TAURA ◽  
R YAMAGUCHI ◽  
S NAKAMA
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Mammary Tumour ◽  
Tumour Cell Lines

scholarly journals Analysis of tumour cell composition in tumours composed of paired mixtures of mammary tumour cell lines

1987 ◽  
Vol 56 (5) ◽  
pp. 561-569 ◽  
Author(s):  
BE Miller ◽  
FR Miller ◽  
DJ Wilburn ◽  
GH Heppner
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Mammary Tumour ◽  
Cell Composition ◽  
Tumour Cell Lines

Screening of Venezuelan medicinal plant extracts for cytostatic and cytotoxic activity against tumour cell lines

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
P Taylor ◽  
M Arsenak ◽  
MJ Abad ◽  
Á Fernández ◽  
R Gonto ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Plant Extracts ◽  
Tumour Cell ◽  
Medicinal Plant Extracts ◽  
Tumour Cell Lines

Cytotoxic activity against tumour cell lines and anti-inflammatory effects of compounds isolated from Xylopia aromatica

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
O Estrada ◽  
L González ◽  
M Mijares ◽  
Á Fernández ◽  
M Ruiz ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Tumour Cell ◽  
Tumour Cell Lines ◽  
Anti Inflammatory
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