scholarly journals Canine osteosarcoma cell lines from patients with differing serum alkaline phosphatase concentrations display no behavioural differencesin vitro

2013 ◽  
Vol 13 (3) ◽  
pp. 166-175 ◽  
Author(s):  
K. E. Holmes ◽  
V. Thompson ◽  
C. M. Piskun ◽  
R. A. Kohnken ◽  
M. K. Huelsmeyer ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Lines ◽  
Serum Alkaline Phosphatase ◽  
Osteosarcoma Cell ◽  
Canine Osteosarcoma

Effects of etoposide alone and in combination with piroxicam on canine osteosarcoma cell lines

2016 ◽  
Vol 218 ◽  
pp. 51-59 ◽  
Author(s):  
S.M. Ong ◽  
K. Saeki ◽  
Y. Tanaka ◽  
R. Nishimura ◽  
T. Nakagawa
Keyword(s):  
Cell Lines ◽  
Osteosarcoma Cell ◽  
Canine Osteosarcoma

Alkaline phosphatase activity from human osteosarcoma cell line SaOS-2: an isoenzyme standard for quantifying skeletal alkaline phosphatase activity in serum.

1989 ◽  
Vol 35 (2) ◽  
pp. 223-229 ◽  
Author(s):  
J R Farley ◽  
E Kyeyune-Nyombi ◽  
N M Tarbaux ◽  
S L Hall ◽  
D D Strong
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Cell Lines ◽  
Alkaline Phosphatase Activity ◽  
Osteosarcoma Cell ◽  
Kinetic Assay ◽  
Human Osteosarcoma ◽  
Alp Activity ◽  
Human Osteosarcoma Cell ◽  
Skeletal Alkaline Phosphatase

Abstract Earlier we described a kinetic assay for quantifying skeletal alkaline phosphatase (ALP) isoenzyme activity in serum. The precision of the assay depends on including ALP standards for the skeletal, hepatic, intestinal, and placental isoenzymes. We wondered whether human osteosarcoma cells could provide an efficient alternative to human bone or Pagetic serum as a source of the skeletal ALP standard. ALP activities prepared from five human osteosarcoma cell lines were compared with a bone-derived ALP standard with respect to heat stability and sensitivity to chemical effectors. Two of the cell lines (SaOS-2 and TE-85) contained ALP activities that resembled the bone-derived standard. We selected SaOS-2 cells for additional evaluation (as a potential source of isoenzyme standard), because they contained 40-50 times more ALP activity than did the TE-85 cells. To include the SaOS-2 cell-derived ALP activity in the quantitative isoenzyme assay, we diluted the enzyme in a solution containing heat-inactivated (i.e., ALP-negative) human serum. Surprisingly, this dilution caused a 60-125% increase in maximum enzyme activity. In the quantitative assay of ALP isoenzyme in serum, the SaOS-2 derived ALP was indistinguishable from the serum skeletal ALP standard, with respect to the above criteria and assay variations. Evidently ALP from SaOS-2 cells is suited as a standard for measuring skeletal ALP activity in this assay.

scholarly journals In vitro and in vivo effects of toceranib phosphate on canine osteosarcoma cell lines and xenograft orthotopic models

2019 ◽  
Vol 18 (1) ◽  
pp. 117-127
Author(s):  
Raquel Sánchez‐Céspedes ◽  
Paolo Accornero ◽  
Silvia Miretti ◽  
Eugenio Martignani ◽  
Francesca Gattino ◽  
...  
Keyword(s):  
Cell Lines ◽  
Osteosarcoma Cell ◽  
Canine Osteosarcoma ◽  
In Vivo Effects

scholarly journals Biologic activity of the novel small molecule STAT3 inhibitor LLL12 against canine osteosarcoma cell lines

2012 ◽  
Vol 8 (1) ◽  
pp. 244 ◽  
Author(s):  
Jason I Couto ◽  
Misty D Bear ◽  
Jiayuh Lin ◽  
Michael Pennel ◽  
Samuel K Kulp ◽  
...  
Keyword(s):  
Cell Lines ◽  
Small Molecule ◽  
Osteosarcoma Cell ◽  
The Novel ◽  
Canine Osteosarcoma ◽  
Biologic Activity

scholarly journals 17-AAG and Apoptosis, Autophagy, and Mitophagy in Canine Osteosarcoma Cell Lines

2016 ◽  
Vol 54 (3) ◽  
pp. 405-412 ◽  
Author(s):  
M. Massimini ◽  
C. Palmieri ◽  
R. De Maria ◽  
M. Romanucci ◽  
D. Malatesta ◽  
...  
Keyword(s):  
Cell Lines ◽  
Osteosarcoma Cell ◽  
Canine Osteosarcoma

scholarly journals Characterization of five newly derived canine osteosarcoma cell lines

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Heather Wilson-Robles ◽  
Kelli Franks ◽  
Roy Pool ◽  
Tasha Miller
Keyword(s):  
Giant Cell ◽  
Cell Lines ◽  
Tumor Development ◽  
Disease Process ◽  
High Rate ◽  
Osteosarcoma Cell ◽  
Canine Osteosarcoma ◽  
Cell Variant

Abstract Background Canine and human osteosarcomas (OS) are notably similar and have a high rate of metastasis. There is a poor understanding of the tumor development process, predisposing causes, and varying levels of aggression among different cell lines. By characterizing newly developed canine osteosarcoma cell lines, treatments for people and pets can be developed. Of the seven subtypes of OS, three are represented in this group: osteoblastic (the most common), fibroblastic, and giant cell variant. To our knowledge, there are no other giant cell variant canine OS cell lines in the published literature and only one canine fibroblastic osteosarcoma cell line. Understanding the differences between the histologic subtypes in dogs will help to guide comparative research. Results Alkaline phosphatase expression was ubiquitous in all cell lines tested and invasiveness was variable between the cell lines tested. Invasiveness and oxidative damage were not correlated with in vivo growth rates, where TOT grew the fastest and had the higher percentage of mice with metastatic lesions. TOL was determined to be the most chemo-resistant during cisplatin chemotherapy while TOM was the most chemo-sensitive. Conclusions Further comparisons and studies using these cell lines may identify a variety of characteristics valuable for understanding the disease process and developing treatments for osteosarcoma in both species. Some of this data was presented as a poster by KMF at the August 5th, 2017 National Veterinary Scholars Program in Bethesda, MA. Characterization of 5 newly generated canine osteosarcoma cell lines. Kelli Franks, Tasha Miller, Heather Wilson-Robles.

Abstract 5637: Investigating tumor intrinsic PD-1/PD-L1 signaling in canine osteosarcoma cell lines as a spontaneous model for human disease

2020 ◽  
Author(s):  
Dziubek Katarzyna ◽  
Mikolaj Kocikowski ◽  
Borek Vojtesek ◽  
David Argyle ◽  
Malgorzata Lisowska ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Disease ◽  
Osteosarcoma Cell ◽  
Canine Osteosarcoma
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