scholarly journals Evaluation of PLGA microspheres with triple regimen on long‐term survival of vascularized composite allograft – an experimental study

2020 ◽  
Vol 33 (4) ◽  
pp. 450-461
Author(s):  
Shoubao Wang ◽  
Yao Xiong ◽  
Yinmin Wang ◽  
Jingting Chen ◽  
Jun Yang ◽  
...  
Keyword(s):  
Experimental Study ◽  
Plga Microspheres ◽  
Term Survival ◽  
Long Term Survival ◽  
Vascularized Composite Allograft ◽  
Triple Regimen

Evaluation of particulate systems supporting tumor cell fractions in a preventive vaccination against intracranial rat glioma

2006 ◽  
Vol 105 (5) ◽  
pp. 745-752 ◽  
Author(s):  
Anne Sapin ◽  
Anne Clavreul ◽  
Emmanuel Garcion ◽  
Jean-Pierre Benoit ◽  
Philippe Menei
Keyword(s):  
Immune Response ◽  
Plasma Membranes ◽  
Plga Microspheres ◽  
Term Survival ◽  
Long Term Survival ◽  
Rat Glioma ◽  
Fischer 344 ◽  
Cell Fractions ◽  
Particulate Systems

Object Irradiated autologous tumor cells are commonly used as a source of antigens in antiglioma vaccinations to activate the immune system. As cell number is often a limiting factor in these cells’ preparation, the aim of the present study was to find a means that can lower the amount of cells required. Among strategies currently developed, adjuvant particulate systems offer a promising means to improve the antitumor immune response. In this study, the authors were interested in evaluating the role of particulate systems containing biodegradable microspheres that carry tumor cell fractions on their surfaces in the induction of a protective immunity in the 9L/Fischer 344 rat glioma model. The efficiency of these particulate systems was compared to that of irradiated 9L cells. Methods Particulate systems composed of poly(d,l-lactide-co-glycolide) (PLGA) microspheres that support 9L cell fractions on their surfaces (cell lysates or plasma membranes) or irradiated 9L cells alone were injected subcutaneously into the flanks of syngeneic Fischer 344 rats. Eighteen days later, the rats were intracranially injected with nonirradiated 9L cells. A study of survival in these animals and an analysis of the resulting immune response were then conducted. For the same amount of protein (50 μg) injected, irradiated 9L cells provided long-term survival in 30% of animals, whereas 9L plasma membranes adsorbed onto PLGA microspheres provided long-term survival in 10% of animals and cell lysates adsorbed onto microspheres provided long-term survival in 0%. Accordingly, particulate systems induced a lower T helper cell Type 1 (Th1) peripheral immune response than irradiated 9L cells. However, greater secretion of Th1 cytokines was observed when particulate systems were used than when cell fractions separated from microspheres were used, indicating the adjuvant property of these particulate systems. Conclusions Particulate systems have adjuvant properties but are still less efficient than irradiated whole tumor cells for vaccinations. Encapsulation of an activating molecule in the microsphere will be the next developmental step in the search for efficient antiglioma vaccinations.

scholarly journals FK506-loaded PLGA nanoparticles improve long-term survival of a vascularized composite allograft in a murine model

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Zheming Cao ◽  
Cheng Li ◽  
Jiqiang He ◽  
Xinlei Sui ◽  
Panfeng Wu ◽  
...  
Keyword(s):  
Murine Model ◽  
Plga Nanoparticles ◽  
Term Survival ◽  
Long Term Survival ◽  
Vascularized Composite Allograft

Long-term survival of free fat grafts in muscle: An experimental study in rats

1996 ◽  
Vol 20 (5) ◽  
pp. 403-408 ◽  
Author(s):  
Jose Guerrerosantos ◽  
Amado Gonzalez-Mendoza ◽  
Yezid Masmela ◽  
Miguel Angel Gonzalez ◽  
Mauro Deos ◽  
...  
Keyword(s):  
Experimental Study ◽  
Term Survival ◽  
Long Term Survival ◽  
Fat Grafts

A single localized dose of enzyme-responsive hydrogel improves long-term survival of a vascularized composite allograft

2014 ◽  
Vol 6 (249) ◽  
pp. 249ra110-249ra110 ◽  
Author(s):  
T. Gajanayake ◽  
R. Olariu ◽  
F. M. Leclere ◽  
A. Dhayani ◽  
Z. Yang ◽  
...  
Keyword(s):  
Term Survival ◽  
Long Term Survival ◽  
Vascularized Composite Allograft

Human thymic epithelial cells maintain long-term survival of clonogenic myeloid and erythroid progenitor cells in vitro

2000 ◽  
Vol 111 (1) ◽  
pp. 363-370 ◽  
Author(s):  
Katsuto Takenaka ◽  
Mine Harada ◽  
Tomoaki Fujisaki ◽  
Koji Nagafuji ◽  
Shinichi Mizuno ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Progenitor Cells ◽  
Thymic Epithelial Cells ◽  
Erythroid Progenitor ◽  
Term Survival ◽  
Long Term Survival ◽  
Erythroid Progenitor Cells

Clinical outcome and long-term survival following radical esophagectomy in the elderly

2001 ◽  
Vol 120 (5) ◽  
pp. A747-A748
Author(s):  
S DRESNER ◽  
A IMMMANUEL ◽  
P LAMB ◽  
S GRIFFIN
Keyword(s):  
Clinical Outcome ◽  
The Elderly ◽  
Radical Esophagectomy ◽  
Term Survival ◽  
Long Term Survival
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