Time from apheresis platelet donation to cold storage: Evaluation of platelet quality and bacterial growth

Transfusion ◽  
2022 ◽  
Author(s):  
Bethany L. Brown ◽  
Stephen J. Wagner ◽  
C. Anne Hapip ◽  
Erin Fischer ◽  
Todd M. Getz ◽  
...  
Keyword(s):  
Cold Storage ◽  
Bacterial Growth

scholarly journals A Lytic Bacteriophage for ControllingPseudomonas lactisin Raw Cow's Milk

2018 ◽  
Vol 84 (18) ◽  
Author(s):  
Chikage Tanaka ◽  
Kohsuke Yamada ◽  
Honami Takeuchi ◽  
Yoshio Inokuchi ◽  
Akiko Kashiwagi ◽  
...  
Keyword(s):  
Cold Storage ◽  
Bacterial Growth ◽  
Raw Milk ◽  
Cow’S Milk ◽  
Content Type ◽  
Cow's Milk ◽  
Milk Processing ◽  
Cell Counts ◽  
Whole Milk ◽  
Raw Cow’S Milk

ABSTRACTThe control of bacterial growth during milk processing is crucial for the quality maintenance of commercial milk and milk products. During a period of cold storage prior to heat treatments, some psychrotrophic bacteria grow and produce extracellular heat-resistant lipases and proteases that cause product defects. The use of lytic bacteriophages (phages) that infect and kill bacteria could be a useful tool for suppressing bacterial growth during this cold storage phase. In this study, we isolated aPseudomonas lactisstrain and a phage from raw cow's milk. Quantitative characterization of the phage was used to elucidate whether this phage was active under low temperatures and neutral pH and whether it was inactivated during pasteurization. Phage titer determination was possible under conditions ranging from pH 4 to 9 and from 3°C to 25°C; the phage was inactivated under pasteurization conditions at 63°C for 30 min. Furthermore, we showed that this phage reduced viable bacterial cell counts in both skim and whole milk. The results of this study represent the potential uses of phages for controlling psychrotrophic bacterial growth in raw cow's milk during cold storage.IMPORTANCESuppression of bacterial growth in raw milk under cold storage is crucial for the quality control of commercially supplied milk. The use of lytic phages as low-cost microbicides is an attractive prospect. Due to strict host specificities, phages must be isolated from the raw milk where the host bacteria are growing. We first isolated theP. lactisbacterial strain and then the phage infecting that strain. Partial phage genomic analysis showed that this is a newly isolated phage, different from any previously reported. This study reports a lytic phage forP. lactis, and we have presented evidence here that this phage reduced viable bacterial cell counts not only in rich medium but also in skim and whole milk. As a result, we have concluded that the phage reported in this study would be useful in milk processing.

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

Survival of partial liver grafts is increased after cold storage in Custodiol-N (HTK-N)

2010 ◽  
Vol 48 (01) ◽  
Author(s):  
Q Liu ◽  
DP Schultze ◽  
H Bruns ◽  
M Zorn ◽  
M Büchler ◽  
...  
Keyword(s):  
Cold Storage

ANALISIS BEBAN PENDINGINAN PADA COLD STORAGE UNTUK PENYIMPANAN DAGING

2019 ◽  
Vol 7 (1) ◽  
pp. 12-22
Author(s):  
Ratu Mutia Fajarani ◽  
Yopi Handoyo ◽  
Raden Hengki Rahmanto
Keyword(s):  
Experimental Method ◽  
Cold Storage ◽  
Freezing Point ◽  
Cooling Load ◽  
Internal Factors ◽  
External Cooling ◽  
Preservation Method ◽  
Load Calculation ◽  
Selection Of

Cooling is the best preservation method than others because the food that has been cooled will remain fresh and will not experience a change in taste, color and aroma, besides all the activities that cause decay will stop so that the cooled food will last longer. (Hartanto, 1984). With the proper cooling engine planning, it can help with spatial adjustments, adjustments to loading, estimation of the power to be used, and budget plans. That is what is commonly called the cooling load calculation. Calculation of cooling load needs to be carried out before planning. This is necessary because the magnitude of the pending load is very influential on the selection of the cooling engine so that the freezing point for preserving food can be accurate. Pendiginan burden is influenced by external and internal factors. With the experimental method, it is obtained the results of the external cooling load as the external cooling load is 11.6 kW, the inner cooling load is 138.8 kW and the performance work coefficient (COP) is 2.

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