Toxic masculinity in red blood cell units? Testosterone therapy in blood donors revisited

Transfusion ◽  
2021 ◽  
Author(s):  
Kelsey Hazegh ◽  
Bradley D. Anawalt ◽  
Larry J. Dumont ◽  
Tamir Kanias
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Donors ◽  
Testosterone Therapy

scholarly journals Prevalence of Red Blood Cell Major Blood Group Antigens and Phenotypes among Omani Blood Donors

2019 ◽  
Vol 34 (6) ◽  
pp. 496-503
Author(s):  
Arwa Z. Al-Riyami ◽  
Ali Al-Marhoobi ◽  
Saif Al-Hosni ◽  
Sabah Al Mahrooqi ◽  
Michael Schmidt ◽  
...  
Keyword(s):  
Blood Cell ◽  
Blood Group ◽  
Red Blood Cell ◽  
Blood Donors ◽  
Blood Group Antigens

Hybrid glycophorin and red blood cell antigen genotyping in Asian American type O blood donors with Mi a phenotype

Transfusion ◽  
2019 ◽  
Vol 59 (12) ◽  
pp. 3767-3775 ◽  
Author(s):  
Xin Lin ◽  
Graciela Rubio ◽  
Jigar Patel ◽  
Sukanta Banerjee ◽  
Tom Frame ◽  
...  
Keyword(s):  
Blood Cell ◽  
Asian American ◽  
Red Blood Cell ◽  
Blood Donors ◽  
Cell Antigen

Platelet and Red Blood Cell Indices in Harris Platelet Syndrome.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3988-3988 ◽  
Author(s):  
Harris V.K. Naina ◽  
Samar Harris
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Donors ◽  
Severe Thrombocytopenia ◽  
Giant Platelets ◽  
Giant Platelet ◽  
Normal Platelet ◽  
Platelet Disorder ◽  
Significant Difference ◽  
Blood Cell Morphology

Abstract Inherited giant platelet disorders are a group of rare disorders characterized by thrombocytopenia, giant platelets and variable bleeding symptoms. Naina et al., described a new giant platelet disorder called Harris Platelet Syndrome (HPS), the most common inherited giant platelet disorder occurring in up to one third of blood donors from north eastern part of Indian subcontinent. HPS is characterized by an autosomal dominant mode of inheritance with normal to severe thrombocytopenia (less than 50x109/L), giant platelets (mean platelet volume more than 10fL) and absent bleeding symptoms with normal platelet aggregation studies. Occasionally abnormalities in red blood cell morphology have been associated with certain giant platelet disorders such as stomatocytosis in Mediterranean Macrothrmboctopenia, dyserythropoiesis in GATA 1 associated macrothrombocytopenia and thalassemia, in X Linked Thrombocytopenia Thalassemia (XLTT). This study was undertaken to analyze the platelet and red blood cell indices in blood donors with Harris Platelet syndrome. A total of 203 blood donors were included in this study, 101 blood donors from northeaster part of India with MPV more than 12fl (normal 7–10fl) and 102 blood donors from southern part of India. Before blood donation, all donors were questioned about a history of bleeding conditions, in either themselves or their relatives. Blood samples were collected in ethylenediaminetetraacetic acid (EDTA). Automated platelet counts were performed using a Coulter STKS (Coulter, Hialeah, Florida) within 2 hours of collection. Peripheral blood smears were examined to confirm thrombocytopenia, giant platelets and red blood cell morphology. There was a significant difference between platelet count (Mean ±SD) 136± 40 Vs 262 ± 53 in southern India (p<0.000). Thirty three donors with HPS had a normal platelet count with MPV more than 12fL. MPV was 13.6±0.13 (range 12 to21.9fL) in donors with HPS and 7.3 ±0.6 (range 6–9.2fl) in southern Indian blood donors. The platelet distribution width (PDW) was 17.4±0.8 in donors with HPS and was 16.38±0.5 in southern India blood donors(p<0.000). Though there was a significant difference between hemoglobin, 13.8 ± 1.0 vs and 14.7± 1.1 (P<0.00), there was no significant difference between RDW, MCV, MCH, MCHC. Peripheral blood smear did not show any obvious red blood cell abnormality, but showed giant platelets and thrombocytopenia. Harris platelet syndrome is associated with normal to severe thrombocytopenia, giant platelets and significant platelet anisocytosis. There was no associated red blood cell abnormalities observed with HPS.

scholarly journals Prevalence of Red Blood Cell Alloantibodies in Blood Donors of Zanjan Province; the Preliminary Report of the North West of Iran

2016 ◽  
Vol 13 (4) ◽  
pp. 2207-2210 ◽  
Author(s):  
Khadijeh Babaei ◽  
Abdolreza Esmaeilzadeh ◽  
Siamak Asadi ◽  
Roya Sohrabi
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Preliminary Report ◽  
Blood Donors ◽  
North West ◽  
The North

scholarly journals Prevalence of irregular red blood cell antibodies among healthy blood donors in South India

2019 ◽  
Vol 4 (2) ◽  
pp. 219
Author(s):  
GDeepthi Krishna ◽  
Deepti Sachan ◽  
Suryatapa Saha ◽  
Raghuram Prasath
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Donors ◽  
South India

scholarly journals Macrophage Polarization Is Impaired in Hemophilia

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 2499-2499
Author(s):  
Lynn M Knowles ◽  
Daniela Lessig ◽  
Martin Bernard ◽  
Eva C Schwarz ◽  
Hermann Eichler ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Cell ◽  
Factor Viii ◽  
Tissue Regeneration ◽  
Red Blood Cell ◽  
Blood Donors ◽  
Macrophage Polarization ◽  
Down Regulation ◽  
Macrophage Differentiation ◽  
Gm Csf

Abstract Macrophages are master regulators of inflammation and wound healing. As such they play an important role in hemophilia, which is commonly associated with delayed tissue regeneration and bleeding-induced joint inflammation. The objective of this study is to determine if macrophage function is deregulated in hemophilia and whether this affects the physiological balance of tissue regeneration and inflammation. For this study, we analyzed monocytes and plasma from a cohort of 26 adult patients with hemophilia A or B that visited our clinic for their annual routine check-up. Patients with acute bleeding events were excluded. The majority of patients had severe forms of hemophilia with factor VIII or IX activity < 1% and, therefore, received prophylactic factor replacement therapy (recombinant or plasmatic) but we also included patients with moderate to mild hemophilia, which were only treated in case of bleeding. Corresponding control samples stemmed from healthy male individuals that we recruited randomly from our blood donor center. To assess macrophage differentiation, we isolated monocytes from the peripheral blood of hemophilia patients as well as healthy controls and began treatment with M-CSF or GM-CSF for 7 days. Macrophage differentiation was confirmed by phase contrast and fluorescence microscopy, which revealed a spread and elongated cell phenotype in donor monocytes in the presence of M-CSF and, to a lesser extent, GM-CSF. Hemophilia monocytes, on the other hand, failed to spread properly in response to either of the cytokines suggesting that macrophage polarization is diminished in patients with reduced activity of coagulation factor VIII or IX. As hemophilia macrophages failed to spread in response to M-CSF, they also failed to express TNFα and CD163, which are macrophage differentiation markers typically induced by M-CSF. In contrast, GM-CSF-induced differentiation was only mildly suppressed suggesting that hemophilia macrophages specifically resist the differentiating stimulus of M-CSF. Consequently, we experienced a significant impairment in M-CSF-induced regenerative macrophage functions such as clot infiltration and red blood cell phagocytosis in hemophilia. Intriguingly, while monocyte invasion was impaired, protein expression in response to M-CSF was regained with respect to CD163 and CD206 after embedding hemophilia monocytes in clotted plasma from healthy blood donors suggesting that a functioning coagulation system has positive effects on regenerative macrophage functions. The inability of hemophilia macrophages to promote M-CSF-mediated signals correlates with a marked down regulation of the M-CSF receptor CSF-1R on hemophilia monocytes as determined in citrated whole blood by flow cytometry. Alongside with a modest reduction of GM-CSF-R, we also detected a substantial reduction of CD163 and Tie2, which are specifically expressed on regenerative monocytes/macrophages, suggesting that clotting deficiencies impair the immune function either directly or indirectly. To further analyze the immune status of hemophilia patients, we performed a cytokine array on plasma samples from hemophiliacs versus healthy blood donors, which revealed the down regulation of a large spectrum of anti-inflammatory and regenerative cytokines in the blood of hemophilia patients. Among the few cytokines upregulated in the blood of hemophiliacs, the adipokine leptin was the most prominent (6-fold, hemophiliac vs. donor). Since leptin has been shown to cause deregulation of the innate immunity, we treated the human monocyte cell line THP1 with recombinant leptin and found a significant inhibitory effect of M-CSF-induced spreading and clot invasion. Therefore, these data suggest that high leptin levels in the blood can reiterate the changes in monocyte function we observed in hemophilia. Together, we conclude that macrophage differentiation is deregulated in hemophilia as a result of resistance towards the cytokine M-CSF. Consequently, hemophilia macrophages are unable to properly perform regenerative functions such as clot invasion and red blood cell phagocytosis. The hemophilic monocyte/macrophage phenotype we describe can be induced by high levels of leptin and mitigated by correcting the clotting dysfunction suggesting a two prone approach to prevent delayed wound healing and persistent inflammation in hemophilia. Disclosures No relevant conflicts of interest to declare.

Multicomponent Collection: The Experience of Regione Lazio

1998 ◽  
Vol 21 (6_suppl) ◽  
pp. 23-25
Author(s):  
G. Girelli ◽  
P. De Fabritiis ◽  
G. Menichella ◽  
R. Serafini ◽  
M.L. Foddai ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Donors ◽  
Blood Collection ◽  
Platelet Concentrates ◽  
Technical Problems ◽  
Blood Banks ◽  
Feasible Alternative ◽  
Fresh Plasma ◽  
Collection Program

Five blood banks of Regione Lazio implemented a multicomponent collection program using apheresis technology. The automated collection of blood components included: red blood cell concentrate and fresh plasma (RBCP), plasma and platelet concentrate (P-PLT), red blood cell and platelet concentrates (RBC-PLT). 334 voluntary blood donors and 30 patients - as autologous donors- were involved. Apheresis collection of RBCP, P - PU, RBC - PLT yielded a standardized product (adequate volume, low residual leucocyte counts, adeguate hematocrit, low platelet contamination) was well tolerated by donors, was performed without technical problems. We conclude that multicomponent collection is a new feasible alternative to conventional whole blood collection.

Bartonella Henselae Survives after the Storage Period of the Red Blood Cell Units.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 2906-2906
Author(s):  
Renata Ferreira Magalhaes ◽  
Luiza Helena Urso Pitassi ◽  
Marizete Salvadego ◽  
Aparecida Machado Moraes ◽  
Maria Lourdes Barjas-Castro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Blood Culture ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Donors ◽  
Storage Period ◽  
Gram Negative ◽  
Chocolate Agar ◽  
Initial Suspension ◽  
Short Period

Abstract Introduction: The genus Bartonella comprises a unique group of emerging, Gram-negative, facultative intracellular bacteria, which can cause Carrión’s disease, trench fever, cat scratch disease and bacillary angiomatosis. Man is a reservoir host of Bartonella species. Ten to 15% of the populations in areas that are hyperendemic for Carrión’s disease are asymptomatic carriers of B. bacilliformis. In addition, positive serologic tests for B. henselae were found in 2 to 6% of Americans, 48% of Swiss, 19% of Germans, and 13.7% of Brazilians. Blood donors can be asymptomatic carrier of Bartonella spp. and the risk for transmission by transfusion should be considered. The aim of this study was to demonstrate that B. henselae remains viable in red blood cell (RBC) units at the end of the storage period. Methods: Two RBC units from healthy blood donors were collected in CPDA1 and each one split into two portions via a sterile connecting device. One bag from each split unit received experimentally B. henselae (Adolpho Lutz Institute, Sao Paulo, Brazil) and the second one served as a control. A brain and heart infusion (BHI) suspension of B. henselae colonies (Houston 1, American Type Culture Collection, Rockville, MD, ATCC 49882T) was used to obtain equivalence with tube 10 of McFarland scale, which determined an initial suspension with approximately 3×109 colony-forming units (CFU)/mL. This bacterial suspension was inoculated (0,33mL of the initial suspension) in one split RBC unit using a Sampling Site Coupler. Thus approximately 5,5×106 CFU of B. henselae were obtained per mL of RBC concentrate. All split units were stored (4 ±2°C) for 35 days. Aliquots were collected weekly using, Sample Site Coupler for, culture in dish with chocolate agar (incubated at 37°C in environment with 5% CO2), samples for blood culture bottles Bact/Alert (BioMérieux, Inc, USA) and Karnovisky medium for future evaluation by transmission electron microscopy. Results: All dishes with chocolate agar culture medium from infected units (seeded on days D0, D7, D14, D21, D28 and D35) presented growth of exuberant and mucoid colonies, after the fourth day of incubation. The electron microscopy from these samples showed typical Gram-negative cell wall in the interior of red blood cells. Samples from infected units presented negative blood culture bottles. All cultures from control units had negative results. Discussion: Bartonella ssp. is fastidious bacteria. Incubation for a short period of time and in standard medium does not allow for B. henselae growth as observed in the bottle culture of this study. Cultivation in blood-enriched media and incubation in environment with high CO2 levels of infected smears resulted positive. In conclusion, this study has demonstrated that B. henselae remain viable in RBC units during the storage period, at 4±2°C. These data reinforce the possibility of infection by transfusion of blood units collected from asymptomatic blood donors. Receptors of blood transfusion many times are or may become immunocompromissed, with risk of developing severe forms of bartonelloses.

scholarly journals Transfusion management of patients with red blood cell antibodies

2013 ◽  
Vol 66 (11-12) ◽  
pp. 491-496 ◽  
Author(s):  
Nevenka Bujandric ◽  
Jasmina Grujic ◽  
Mirjana Krga-Milanovic
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
High Frequency ◽  
Blood Donors ◽  
Blood Compatibility ◽  
Blood Group System ◽  
System 2 ◽  
Special Challenge ◽  
Clinically Significant ◽  
D 12

Introduction. Red blood cell antibodies may cause a positive result of pre-transfusion blood compatibility testing (crossmatch test). It can be a problem to provide suitable blood units for patients with clinically significant antibodies to high-frequency antigens as well as for those with multiple alloantibody specificities. This study was aimed at identifying transfused patients in the population of South-Backa who had developed clinically significant red blood cell alloantibodies. Material and methods. We analyzed the records of crossmatch results and antibody screening performed at the Blood Transfusion Institute of Vojvodina during 2012. Results. Antibodies were found in 103 patients: A) 63 patients with single antibodies: 1) 16 with antibodies of unknown specificity (3 autoantibodies, 13 alloantibodies); 2) 39 with clinically significant antibodies (23 from Rh system (2 anti-C, 2 anti-D, 12 anti-E, 7 anti-c), 4 anti-K, 3 anti-Fya, 7 anti-Jka, 2 anti-S); 3) 8 with usually not significant antibodies (6 anti-M, 1 anti-A1, 1 anti- Cw); B) 40 patients developed multiple antibodies: 1) all patients had at least one clinically significant antibody from various blood group system (44 Rh, 13 Kell, 7 Kidd, 7 MNSs (S, s)); 2) 3 patients had usually not significant antibodies (1 Lewis, 2 Lutheran); 3) 3 patients occasionally had clinically significant antibody (3 anti- Yta); 4) 3 patients had antibodies of unknown specificity (2 autoantibodies, 1alloantibody). Antibodies detected in the majority of patients (65-63.1%) had a specificity of Rh and/or the Kell system. Conclusions. The main goal of pre-transfusion blood compatibility testing is to detect clinically significant antibodies. The provision of antigen negative blood units for those patients is a special challenge for blood establishments. Database with a sufficient number of typed blood donors can help to resolve this problem.

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