Detection of Babesia RNA and DNA in whole blood samples from US blood donations

An ever-increasing need exists within the forensic laboratories to develop analytical processes for the qualitative and quantitative determination of a broad spectrum of new psychoactive substances. Phenylethylamine derivatives are among the major classes of psychoactive substances available on the global market and include both amphetamine analogues and synthetic cathinones. In this work, an ultra-high-performance liquid chromatography-positive ion electrospray ionization tandem mass spectrometric method (UHPLC-ESI-MS/MS) has been developed and fully validated for the determination of 19 psychoactive substances, including nine amphetamine-type stimulants and 10 synthetic cathinone derivatives, in premortem and postmortem whole blood. The assay was based on the use of 1 mL premortem or postmortem whole blood, following solid phase extraction prior to the analysis. The separation was achieved on a Poroshell 120 EC-C18 analytical column with a gradient mobile phase of 0.1% formic acid in acetonitrile and 0.1% formic acid in water in 9 min. The dynamic multiple reaction monitoring used in this work allowed for limit of detection (LOD) and lower limit of quantitation (LOQ) values of 0.5 and 2 ng mL−1, respectively, for all analytes both in premortem and postmortem whole blood samples. A quadratic calibration model was used for the 12 quantitative analytes over the concentration range of 20–2000 ng mL−1, and the method was shown to be precise and accurate both in premortem and postmortem whole blood. The method was applied to the analysis of real cases and proved to be a valuable tool in forensic and clinical toxicology.

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Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

STAR Protocols ◽

10.1016/j.xpro.2021.100311 ◽

2021 ◽

Vol 2 (1) ◽

pp. 100311

Author(s):

Daniella C. Terenzi ◽

Ehab Bakbak ◽

Justin Z. Trac ◽

Mohammad Al-Omran ◽

Adrian Quan ◽

...

Keyword(s):

Whole Blood ◽

Progenitor Cell ◽

Blood Samples ◽

Human Whole Blood ◽

Isolation And Characterization ◽

Whole Blood Samples

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Detection of melanoma cells in whole blood samples using spectral imaging and optical clearing

IEEE Journal of Selected Topics in Quantum Electronics ◽

10.1109/jstqe.2020.3047437 ◽

2020 ◽

pp. 1-1

Author(s):

Polina A. Dyachenko Timoshina ◽

Leonid E. Dolotov ◽

Ekaterina N. Lazareva ◽

Anastasiia A. Kozlova ◽

Olga A. Inozemtseva ◽

...

Keyword(s):

Whole Blood ◽

Spectral Imaging ◽

Melanoma Cells ◽

Blood Samples ◽

Optical Clearing ◽

Whole Blood Samples

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The distribution of BrKα/RbKα peak intensity ratio in human whole blood samples

Biological Trace Element Research ◽

10.1007/bf02911520 ◽

1994 ◽

Vol 42 (3) ◽

pp. 231-241 ◽

Cited By ~ 2

Author(s):

C. Shenberg ◽

S. Spiegel ◽

S. Chaitchik ◽

P. Jordan ◽

M. Kitzis ◽

...

Keyword(s):

Whole Blood ◽

Intensity Ratio ◽

Peak Intensity Ratio ◽

Blood Samples ◽

Human Whole Blood ◽

Peak Intensity ◽

Whole Blood Samples

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Interlaboratory comparison of results of erythrocyte protoporphyrin analysis.

Clinical Chemistry ◽

10.1093/clinchem/24.12.2135 ◽

1978 ◽

Vol 24 (12) ◽

pp. 2135-2138 ◽

Cited By ~ 5

Author(s):

K W Jackson

Keyword(s):

Acetic Acid ◽

Hydrochloric Acid ◽

Ethyl Acetate ◽

Disease Control ◽

Whole Blood ◽

Interlaboratory Comparison ◽

Direct Reading ◽

Blood Samples ◽

Erythrocyte Protoporphyrin ◽

Whole Blood Samples

Abstract Each of 65 laboratories analyzed 10 whole-blood samples for erythrocyte protoporphyrin by one or more of several analytical procedures. These procedures were of two types: (a) extraction of protoporphyrin from the erythrocytes into ethyl acetate/acetic acid, re-extraction into hydrochloric acid, and fluorometric measurement; or (b) direct reading in a portable fluorometer (hematofluorometer), with no pretreatment of the blood sample. Interlaboratory correlation was generally poor, especially between laboratories using extraction procedures. Hematofluorometric results intercorrelated better, but they had a low bias as compared to the extraction approach. Nationwide standardization of the test is required to assure satisfactory interlaboratory performance and to identify laboratories whose results are sufficiently accurate to be used for interpretations according to guidelines set forth by the Center for Disease Control for erythrocyte protoporphyrin testing.

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Optimized protocol for the extraction of RNA and DNA from frozen whole blood sample stored in a single EDTA tube

Scientific Reports ◽

10.1038/s41598-021-96567-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Hirotaka Yamagata ◽

Ayumi Kobayashi ◽

Ryouichi Tsunedomi ◽

Tomoe Seki ◽

Masaaki Kobayashi ◽

...

Keyword(s):

Whole Blood ◽

Ethylenediaminetetraacetic Acid ◽

Rna Extraction ◽

Basic Research ◽

Rna Degradation ◽

Disodium Salt ◽

Water Bath ◽

High Quality ◽

Blood Samples ◽

Rna And Dna

AbstractCryopreservation of whole blood is useful for DNA collection, and clinical and basic research. Blood samples in ethylenediaminetetraacetic acid disodium salt (EDTA) tubes stored at − 80 °C are suitable for DNA extraction, but not for high-quality RNA extraction. Herein, a new methodology for high-quality RNA extraction from human blood samples is described. Quickly thawing frozen whole blood on aluminum blocks at room temperature could minimize RNA degradation, and improve RNA yield and quality compared with thawing the samples in a 37 °C water bath. Furthermore, the use of the NucleoSpin RNA kit increased RNA yield by fivefold compared with the PAXgene Blood RNA Kit. Thawing blood samples on aluminum blocks significantly increased the DNA yield by ~ 20% compared with thawing in a 37 °C water bath or on ice. Moreover, by thawing on aluminum blocks and using the NucleoSpin RNA and QIAamp DNA Blood kits, the extraction of RNA and DNA of sufficient quality and quantity was achieved from frozen EDTA whole blood samples that were stored for up to 8.5 years. Thus, extracting RNA from frozen whole blood in EDTA tubes after long-term storage is feasible. These findings may help advance gene expression analysis, as well as biomarker research for various diseases.

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