Comparison of the platelet activation status of single‐donor platelets obtained with two different cell separator technologies

Transfusion ◽  
2020 ◽  
Author(s):  
Daniel Millar ◽  
Chelsea Hayes ◽  
Jessica Jones ◽  
Ellen Klapper ◽  
Joel N. Kniep ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Cell Separator ◽  
Single Donor ◽  
Activation Status

scholarly journals Monocyte Functional Responsiveness After PSGL-1–Mediated Platelet Adhesion Is Dependent on Platelet Activation Status

2008 ◽  
Vol 28 (8) ◽  
pp. 1491-1498 ◽  
Author(s):  
Stylianos Bournazos ◽  
Jillian Rennie ◽  
Simon P. Hart ◽  
Keith A.A. Fox ◽  
Ian Dransfield
Keyword(s):  
Platelet Activation ◽  
Platelet Adhesion ◽  
Functional Responsiveness ◽  
Activation Status

scholarly journals Reduced miR-26b Expression in Megakaryocytes and Platelets Contributes to Elevated Level of Platelet Activation Status in Sepsis

2020 ◽  
Vol 21 (3) ◽  
pp. 866 ◽  
Author(s):  
Bernadett Szilágyi ◽  
Zsolt Fejes ◽  
Szilárd Póliska ◽  
Marianna Pócsi ◽  
Zsolt Czimmerer ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Mirna Expression ◽  
Mrna Level ◽  
Severe Infection ◽  
Mrna Levels ◽  
Rna Seq ◽  
Calpain Inhibition ◽  
The Impact ◽  
Lps Treatment ◽  
Activation Status

In sepsis, platelets may become activated via toll-like receptors (TLRs), causing microvascular thrombosis. Megakaryocytes (MKs) also express these receptors; thus, severe infection may modulate thrombopoiesis. To explore the relevance of altered miRNAs in platelet activation upon sepsis, we first investigated sepsis-induced miRNA expression in platelets of septic patients. The effect of abnormal Dicer level on miRNA expression was also evaluated. miRNAs were profiled in septic vs. normal platelets using TaqMan Open Array. We validated platelet miR-26b with its target SELP (P-selectin) mRNA levels and correlated them with clinical outcomes. The impact of sepsis on MK transcriptome was analyzed in MEG-01 cells after lipopolysaccharide (LPS) treatment by RNA-seq. Sepsis-reduced miR-26b was further studied using Dicer1 siRNA and calpain inhibition in MEG-01 cells. Out of 390 platelet miRNAs detected, there were 121 significantly decreased, and 61 upregulated in sepsis vs. controls. Septic platelets showed attenuated miR-26b, which were associated with disease severity and mortality. SELP mRNA level was elevated in sepsis, especially in platelets with increased mean platelet volume, causing higher P-selectin expression. Downregulation of Dicer1 generated lower miR-26b with higher SELP mRNA, while calpeptin restored miR-26b in MEG-01 cells. In conclusion, decreased miR-26b in MKs and platelets contributes to an increased level of platelet activation status in sepsis.

Evaluation of Platelet Concentrates with the New Dideco Blood Cell Separator: Excel

1993 ◽  
Vol 16 (5_suppl) ◽  
pp. 130-134 ◽  
Author(s):  
F. Schooneman
Keyword(s):  
Blood Cell ◽  
Platelet Activation ◽  
Complement Activation ◽  
The Other ◽  
Platelet Concentrates ◽  
Cell Separator ◽  
Good Compromise ◽  
Blood Cell Separator ◽  
The Future

The Excel Cell Separator produced by Dideco represents a good compromise between automation and Platelet efficiency. Platelet activation is a phenomenon that we can find with the concentrates of all cell separators. Although, the complement activation of Excel is extremely lower in comparison with the same data obtained using all the other cell separators, the 5-day storage is acceptable. It will be important to research the bacteriological controls. Like the pH of the platelet bags, because in certain cases it was too low. For the future it will be interesting to study the possibility to predict the quantity of platelets collected having a system that is adaptable with the donor parameters.

Automated assessment of the neutrophil and platelet activation status in patients with essential thrombocythemia

Platelets ◽  
2011 ◽  
Vol 23 (5) ◽  
pp. 336-343 ◽  
Author(s):  
Eduardo Arellano-Rodrigo ◽  
Alberto Alvarez-Larrán ◽  
Juan Carlos Reverter ◽  
Neus Villamor ◽  
Josep Maria Jou ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Essential Thrombocythemia ◽  
Automated Assessment ◽  
Activation Status

Description and use of the CS-3000 blood cell separator for single- donor platelet collection

Transfusion ◽  
1983 ◽  
Vol 23 (3) ◽  
pp. 190-196 ◽  
Author(s):  
DH Buchholz ◽  
JH Porten ◽  
JE Menitove ◽  
L Rzad ◽  
RR Bucheger ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Separator ◽  
Single Donor ◽  
Blood Cell Separator

scholarly journals Desialylation of Platelets by Pneumococcal Neuraminidase A Induces ADP-Dependent Platelet Hyperreactivity

2018 ◽  
Vol 86 (10) ◽  
Author(s):  
Vesla Kullaya ◽  
Marien I. de Jonge ◽  
Jeroen D. Langereis ◽  
Christa E. van der Gaast-de Jongh ◽  
Christian Büll ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Sialic Acid ◽  
Platelet Aggregation ◽  
Platelet Activation ◽  
Ex Vivo ◽  
Neuraminidase Inhibitors ◽  
Platelet Surface ◽  
Content Type ◽  
Fibrinogen Binding ◽  
Activation Status

ABSTRACTPlatelets are increasingly recognized to play a role in the complications ofStreptococcus pneumoniaeinfections.S. pneumoniaeexpresses neuraminidases, which may alter glycans on the platelet surface. In the present study, we investigated the capability of pneumococcal neuraminidase A (NanA) to remove sialic acid (desialylation) from the platelet surface, the consequences for the platelet activation status and reactivity, and the ability of neuraminidase inhibitors to prevent these effects. Our results show that soluble NanA induces platelet desialylation. Whereas desialylation itself did not induce platelet activation (P-selectin expression and platelet fibrinogen binding), platelets became hyperreactive toex vivostimulation by ADP and cross-linked collagen-related peptide (CRP-XL). Platelet aggregation with leukocytes also increased. These processes were dependent on the ADP pathway, as inhibitors of the pathway (apyrase and ticagrelor) abrogated platelet hyperreactivity. Inhibition of NanA-induced platelet desialylation by neuraminidase inhibitors (e.g., oseltamivir acid) also prevented the platelet effects of NanA. Collectively, our findings show that soluble NanA can desialylate platelets, leading to platelet hyperreactivity, which can be prevented by neuraminidase inhibitors.

Collection of WBC-reduced single-donor PLT concentrates with a new blood cell separator: results of a multicenter study

Transfusion ◽  
2003 ◽  
Vol 43 (8) ◽  
pp. 1107-1114 ◽  
Author(s):  
Roger Moog ◽  
Thomas Zeiler ◽  
Hans-Gert Heuft ◽  
Berckard Stephan ◽  
Eike G. Fischer ◽  
...  
Keyword(s):  
Blood Cell ◽  
Multicenter Study ◽  
Cell Separator ◽  
Single Donor ◽  
Blood Cell Separator

Effects of plasma nitric oxide levels on platelet activation in single donor apheresis and random donor concentrates

2007 ◽  
Vol 36 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Demet İren Büyükkağnıcı ◽  
Osman İlhan ◽  
Güzin Özelçi Kavas ◽  
Önder Arslan ◽  
Mutlu Arat ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Platelet Activation ◽  
Single Donor
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