Differential red blood cell age fractionation and Band 3 phosphorylation distinguish two different subtypes of warm autoimmune hemolytic anemia

Transfusion ◽  
2020 ◽  
Vol 60 (8) ◽  
pp. 1856-1866
Author(s):  
Evgenia M. Bloch ◽  
Haley A. Branch ◽  
Darinka Sakac ◽  
Regina M. Leger ◽  
Donald R. Branch
Keyword(s):  
Blood Cell ◽  
Hemolytic Anemia ◽  
Red Blood Cell ◽  
Autoimmune Hemolytic Anemia ◽  
Band 3 ◽  
Cell Age

Interleukin-10–mediated regulatory T-cell responses to epitopes on a human red blood cell autoantigen

Blood ◽  
2002 ◽  
Vol 100 (13) ◽  
pp. 4529-4536 ◽  
Author(s):  
Andrew M. Hall ◽  
Frank J. Ward ◽  
Mark A. Vickers ◽  
Lisa-Marie Stott ◽  
Stanislaw J. Urbaniak ◽  
...  
Keyword(s):  
T Cell ◽  
Blood Cell ◽  
Hemolytic Anemia ◽  
Red Blood Cell ◽  
Regulatory T Cell ◽  
Autoimmune Hemolytic Anemia ◽  
T Cell Responses ◽  
Interleukin 10 ◽  
Cell Responses ◽  
Immune Mediated

Regulatory T cells have been shown to control animal models of immune-mediated pathology by inhibitory cytokine production, but little is known about such cells in human disease. Here we characterize regulatory T-cell responses specific for a human red blood cell autoantigen in patients with warm-type autoimmune hemolytic anemia. Peripheral blood mononuclear cells from patients with autoimmune hemolytic anemia were found either to proliferate and produce interferon-γ or to secrete the regulatory cytokine interleukin 10 when stimulated in vitro with a major red blood cell autoantigen, the RhD protein. Flow cytometric analysis confirmed that the majority of the responding cells were of the CD4+phenotype. Serial results from individual patients demonstrated that this bias toward proliferative or interleukin-10 responses was unstable over time and could reverse in subsequent samples. Epitope mapping studies identified peptides from the sequence of the autoantigen that preferentially induced interleukin-10 production, rather than proliferation, and demonstrated that many contain naturally processed epitopes. Responses to such peptides suppressed T-cell proliferation against the RhD protein, an inhibition that was mediated largely by interleukin 10 and dependent on cytotonic T lymphocyte–associated antigen (CTLA-4) costimulation. Antigenic peptides with the ability to stimulate specific regulatory cells may represent a new class of therapeutic agents for immune-mediated disease.

scholarly journals Autoantibodies and red blood cell membrane proteins in a case of canine autoimmune hemolytic anemia.

1991 ◽  
Vol 53 (1) ◽  
pp. 19-21
Author(s):  
Shuichi TSUCHIDA ◽  
Reiko USUI ◽  
Ryouichi USUI ◽  
Umetaro MURAMATSU ◽  
Hiroyasu EJIMA ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Blood Cell ◽  
Hemolytic Anemia ◽  
Red Blood Cell ◽  
Autoimmune Hemolytic Anemia ◽  
Red Blood Cell Membrane ◽  
Cell Membrane Proteins

scholarly journals Isotachophoretic analysis of supernatant of red blood cell suspension treated with Dispase and serum in autoimmune hemolytic anemia.

1986 ◽  
Vol 30 (4) ◽  
pp. 247-250
Author(s):  
Eiji Kajii ◽  
Yuhji Yamaguchi ◽  
Juichi Ueki ◽  
Yasusada Miura ◽  
Haruhiro Yoshida ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Suspension ◽  
Hemolytic Anemia ◽  
Red Blood Cell ◽  
Autoimmune Hemolytic Anemia

Cut-off value of red-blood-cell-bound IgG for the diagnosis of Coombs-negative autoimmune hemolytic anemia

2009 ◽  
Vol 84 (2) ◽  
pp. 98-101 ◽  
Author(s):  
Toyomi Kamesaki ◽  
Takashi Oyamada ◽  
Mitsuhiro Omine ◽  
Keiya Ozawa ◽  
Eiji Kajii
Keyword(s):  
Blood Cell ◽  
Hemolytic Anemia ◽  
Red Blood Cell ◽  
Autoimmune Hemolytic Anemia

scholarly journals Requirement of IL-5 for induction of autoimmune hemolytic anemia in anti-red blood cell autoantibody transgenic mice

1999 ◽  
Vol 11 (6) ◽  
pp. 995-1000 ◽  
Author(s):  
Toshio Sakiyama ◽  
Koichi Ikuta ◽  
Sazuku Nisitani ◽  
Kiyoshi Takatsu ◽  
Tasuku Honjo
Keyword(s):  
Transgenic Mice ◽  
Blood Cell ◽  
Hemolytic Anemia ◽  
Red Blood Cell ◽  
Autoimmune Hemolytic Anemia

scholarly journals Autoimmune Hemolytic Anemia and Red Blood Cell Autoantibodies

2015 ◽  
Vol 139 (11) ◽  
pp. 1455-1458 ◽  
Author(s):  
Erin Quist ◽  
Scott Koepsell
Keyword(s):  
Blood Cell ◽  
Clinical Significance ◽  
Hemolytic Anemia ◽  
Red Blood Cell ◽  
Autoimmune Hemolytic Anemia ◽  
Underlying Disease ◽  
Laboratory Findings ◽  
Life Threatening ◽  
The Common ◽  
Ancillary Studies

Autoimmune hemolytic anemia is a rare disorder caused by autoreactive red blood cell (RBC) antibodies that destroy RBCs. Although autoimmune hemolytic anemia is rare, RBC autoantibodies are encountered frequently and can complicate transfusion workups, impede RBC alloantibody identification, delay distribution of compatible units, have variable clinical significance that ranges from benign to life-threatening, and may signal an underlying disease or disorder. In this review, we discuss the common presenting features of RBC autoantibodies, laboratory findings, ancillary studies that help the pathologist investigate the clinical significance of autoantibodies, and how to provide appropriate patient care and consultation for clinical colleagues. Pathologists must be mindful of, and knowledgeable about, this entity because it not only allows for direct clinical management but also can afford an opportunity to preemptively treat an otherwise silent malignancy or disorder.

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