scholarly journals How do I establish a stool bank for fecal microbiota transplantation within the blood‐ and tissue transplant service?

Transfusion ◽  
2020 ◽  
Vol 60 (6) ◽  
pp. 1135-1141 ◽  
Author(s):  
Maja Skov Kragsnaes ◽  
Anna Christine Nilsson ◽  
Jens Kjeldsen ◽  
Hanne Marie Holt ◽  
Kristina Fruerlund Rasmussen ◽  
...  
Keyword(s):  
Fecal Microbiota Transplantation ◽  
Fecal Microbiota ◽  
Tissue Transplant ◽  
Stool Bank

scholarly journals Prospective Laboratory Evaluation of Fecal Microbiota Transplantation Donors: Results From an International Public Stool Bank

2016 ◽  
Vol 3 (suppl_1) ◽  
Author(s):  
Kelly Ling ◽  
Emily Koelsch ◽  
Nancy Dubois ◽  
Kelsey O'Brien ◽  
Zachery Stoltzner ◽  
...  
Keyword(s):  
Fecal Microbiota Transplantation ◽  
Fecal Microbiota ◽  
Laboratory Evaluation ◽  
Stool Bank

Sa1064 The International Public Stool Bank: A Scalable Model for Standardized Screening and Processing of Donor Stool for Fecal Microbiota Transplantation

2015 ◽  
Vol 148 (4) ◽  
pp. S-211 ◽  
Author(s):  
Mark B. Smith ◽  
Zain Kassam ◽  
James Burgess ◽  
Allison R. Perrotta ◽  
Laura J. Burns ◽  
...  
Keyword(s):  
Fecal Microbiota Transplantation ◽  
Fecal Microbiota ◽  
Standardized Screening ◽  
Stool Bank

scholarly journals 16S rRNA sequencing of samples from universal stool bank donors

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Marina Santiago ◽  
Scott W. Olesen
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
Fecal Microbiota Transplantation ◽  
Fecal Microbiota ◽  
16S Rrna Sequencing ◽  
Clostridioides Difficile ◽  
Rrna Sequencing ◽  
Health And Disease ◽  
Gut Microbial Community ◽  
Stool Bank

Abstract Objectives Universal stool banks provide stool to physicians for use in treating recurrent Clostridioides difficile infection via fecal microbiota transplantation. Stool donors providing the material are rigorously screened for diseases and disorders with a potential microbiome etiology, and they are likely healthier than the controls in most microbiome datasets. 16S rRNA sequencing was performed on samples from a selection of stool donors at a large stool bank, OpenBiome, to characterize their gut microbial community and to compare samples across different timepoints and sequencing runs. Data description 16S rRNA sequencing was performed on 200 samples derived from 170 unique stool donations from 86 unique donors. Samples were sequenced on 11 different sequencing runs. We are making this data available because rigorously screened, likely very healthy stool donors may be useful for characterizing and understanding microbial community differences across different populations and will help shed light into the how the microbiome community promotes health and disease.

scholarly journals Stool banking for fecal microbiota transplantation: methods and operations at a large stool bank

2020 ◽  
Author(s):  
Justin Chen ◽  
Amanda Zaman ◽  
Bharat Ramakrishna ◽  
Scott W Olesen
Keyword(s):  
Median Time ◽  
Fecal Microbiota Transplantation ◽  
Improve Patient Care ◽  
Fecal Microbiota ◽  
Screening Guidelines ◽  
Donor Screening ◽  
Clostridioides Difficile ◽  
Clostridioides Difficile Infection ◽  
Improve Patient ◽  
Stool Bank

Objectives: Fecal microbiota transplantation (FMT) is a recommended therapy for recurrent Clostridioides difficile infection and is being investigated as a potential therapy for dozens of microbiome-mediated indications. Stool banks centralize FMT donor screening and FMT material preparation with the goal of improving the safety, quality, convenience, and accessibility of FMT material. Although there are published consensuses on donor screening guidelines, there are few reports about the implementation of those guidelines in functioning stool banks. Methods: To help inform consensus standards with data gathered from real-world settings and, in turn, to improve patient care, here we describe the general methodology used in 2018 by OpenBiome, a large stool bank, and its outputs in that year. Results: In 2018, the stool bank received 7,536 stool donations from 210 donors, a daily average of 20.6 donations, and processed 4,271 of those donations into FMT preparations. The median time a screened and enrolled stool donor actively donated stool was 5.8 months. The median time between the manufacture of an FMT preparation and its shipment to a hospital or physician was 8.9 months. Half of the stool bank's partner hospitals and physicians ordered an average of 0.75 or fewer FMT preparations per month. Conclusions: Further knowledge sharing should help inform refinements of stool banking guidelines and best practices. 

scholarly journals STRUCTURING A FECAL MICROBIOTA TRANSPLANTATION CENTER IN A UNIVERSITY HOSPITAL IN BRAZIL

2020 ◽  
Vol 57 (4) ◽  
pp. 434-458
Author(s):  
Daniel Antônio de Albuquerque TERRA ◽  
Eduardo Garcia VILELA ◽  
Rodrigo Otávio Silveira SILVA ◽  
Laiane Alves LEÃO ◽  
Karine Sampaio LIMA ◽  
...  
Keyword(s):  
Clinical Evaluation ◽  
Fecal Microbiota Transplantation ◽  
Fecal Microbiota ◽  
Donor Selection ◽  
University Hospital ◽  
Fecal Microbiota Transplant ◽  
Use Of Resources ◽  
Transplant Center ◽  
Transplantation Center ◽  
Stool Bank

ABSTRACT BACKGROUND: Fecal microbiota transplantation (FMT) is an important therapeutic option for recurrent or refractory Clostridioides difficile infection, being a safe and effective method. Initial results suggest that FMT also plays an important role in other conditions whose pathogenesis involves alteration of the intestinal microbiota. However, its systematized use is not widespread, especially in Brazil. In the last decade, multiple reports and several cases emerged using different protocols for FMT, without standardization of methods and with variable response rates. In Brazil, few isolated cases of FMT have been reported without the implantation of a Fecal Microbiota Transplantation Center (FMTC). OBJECTIVE: The main objective of this study is to describe the process of implanting a FMTC with a stool bank, in a Brazilian university hospital for treatment of recurrent and refractory C. difficile infection. METHODS: The center was structured within the criteria required by international organizations such as the Food and Drug Administration, the European Fecal Microbiota Transplant Group and in line with national epidemiological and regulatory aspects. RESULTS: A whole platform involved in structuring a transplant center with stool bank was established. The criteria for donor selection, processing and storage of samples, handling of recipients before and after the procedure, routes of administration, short and long-term follow-up of transplant patients were determined. Donor selection was conducted in three stages: pre-screening, clinical evaluation and laboratory screening. Most of the candidates were excluded in the first (75.4%) and second stage (72.7%). The main clinical exclusion criteria were: recent acute diarrhea, overweight (body mass index ≥25 kg/m2) and chronic gastrointestinal disorders. Four of the 134 candidates were selected after full screening, with a donor detection rate of 3%. CONCLUSION: The implantation of a transplant center, unprecedented in our country, allows the access of patients with recurrent or refractory C. difficile infection to innovative, safe treatment, with a high success rate and little available in Brazil. Proper selection of qualified donors is vital in the process of implementing a FMTC. The rigorous clinical evaluation of donors allowed the rational use of resources. A transplant center enables treatment on demand, on a larger scale, less personalized, with more security and traceability. This protocol provides subsidies for conducting FMT in emerging countries.

scholarly journals Stool Banking for Fecal Microbiota Transplantation: Methods and Operations at a Large Stool Bank

2021 ◽  
Vol 11 ◽  
Author(s):  
Justin Chen ◽  
Amanda Zaman ◽  
Bharat Ramakrishna ◽  
Scott W. Olesen
Keyword(s):  
Median Time ◽  
Fecal Microbiota Transplantation ◽  
Improve Patient Care ◽  
Fecal Microbiota ◽  
Screening Guidelines ◽  
Donor Screening ◽  
Clostridioides Difficile ◽  
Improve Patient ◽  
Material Preparation ◽  
Stool Bank

ObjectivesFecal microbiota transplantation (FMT) is a recommended therapy for recurrent Clostridioides difficile infection and is being investigated as a potential therapy for dozens of microbiota-mediated indications. Stool banks centralize FMT donor screening and FMT material preparation with the goal of expanding access to FMT material while simultaneously improving its safety, quality, and convenience. Although there are published consensuses on donor screening guidelines, there are few reports about the implementation of those guidelines in functioning stool banks.MethodsTo help inform consensus standards with data gathered from real-world settings and, in turn, to improve patient care, here we describe the general methodology used in 2018 by OpenBiome, a large stool bank, and its outputs in that year.ResultsIn 2018, the stool bank received 7,536 stool donations from 210 donors, a daily average of 20.6 donations, and processed 4,271 of those donations into FMT preparations. The median time a screened and enrolled stool donor actively donated stool was 5.8 months. The median time between the manufacture of an FMT preparation and its shipment to a hospital or physician was 8.9 months. Half of the stool bank’s partner hospitals and physicians ordered an average of 0.75 or fewer FMT preparations per month.ConclusionsFurther knowledge sharing should help inform refinements of stool banking guidelines and best practices.

Sign in / Sign up

Export Citation Format

Share Document