scholarly journals Whole blood for postpartum hemorrhage: early experience at two institutions

Transfusion ◽  
2020 ◽  
Vol 60 (S3) ◽  
Author(s):  
David S. Morris ◽  
Maxwell A. Braverman ◽  
Jessica Corean ◽  
John C. Myers ◽  
Elly Xenakis ◽  
...  
Keyword(s):  
Whole Blood ◽  
Postpartum Hemorrhage ◽  
Early Experience

scholarly journals Early experience with transfusing low titer group O whole blood in the pre‐hospital setting in Israel

Transfusion ◽  
2020 ◽  
Vol 60 (S3) ◽  
Author(s):  
Roy Nadler ◽  
Avishai M. Tsur ◽  
Mark H. Yazer ◽  
Eilat Shinar ◽  
Tzadok Moshe ◽  
...  
Keyword(s):  
Whole Blood ◽  
Hospital Setting ◽  
Early Experience ◽  
Titer Group

scholarly journals Early Experience of Intraoperative Autotransfusion

1988 ◽  
Vol 81 (7) ◽  
pp. 389-391 ◽  
Author(s):  
A J Wilson ◽  
B J Cuddigan ◽  
A P Wyatt
Keyword(s):  
Vascular Surgery ◽  
Whole Blood ◽  
Early Experience ◽  
Operative Field ◽  
Simple Method ◽  
Intraoperative Autotransfusion ◽  
Major Vascular Surgery

Intraoperative autotransfusion of whole blood was performed using the Solcotrans device in 45 patients undergoing major vascular surgery or other procedures in which there was a clean operative field. Overall, 60% of the blood shed during these operations was reinfused. Approximately 50% of patients avoided the potential complications of homologous transfusion. There were no complications attributable to the use of this device although in a sample of 13 patients, 30% of the erythrocytes were haemolysed and there were other minor haematological abnormalities. In conclusion this device provides a simple method of autotransfusing 2 or 3 litres of blood aspirated from an uncontaminated operative field.

First report of using low‐titer cold‐stored type O whole blood in massive postpartum hemorrhage

Transfusion ◽  
2019 ◽  
Vol 59 (10) ◽  
pp. 3089-3092 ◽  
Author(s):  
Timothy M. Bahr ◽  
Tara L. DuPont ◽  
David S. Morris ◽  
Spencer E. Pierson ◽  
Michael Sean Esplin ◽  
...  
Keyword(s):  
Whole Blood ◽  
Postpartum Hemorrhage ◽  
First Report

Fine Structure of Platelet Interaction with a New Microcrystalline Collagen Preparation

1974 ◽  
Vol 32 ◽  
pp. 58-59
Author(s):  
W. H. Zucker ◽  
R. G. Mason
Keyword(s):  
Fine Structure ◽  
Platelet Aggregation ◽  
Hydrochloric Acid ◽  
Whole Blood ◽  
Platelet Adhesion ◽  
Hemostatic Agent ◽  
Native Collagen ◽  
Fibrillar Morphology ◽  
Clinical Interest ◽  
New Form

Platelet adhesion initiates platelet aggregation and is an important component of the hemostatic process. Since the development of a new form of collagen as a topical hemostatic agent is of both basic and clinical interest, an ultrastructural and hematologic study of the interaction of platelets with the microcrystalline collagen preparation was undertaken.In this study, whole blood anticoagulated with EDTA was used in order to inhibit aggregation and permit study of platelet adhesion to collagen as an isolated event. The microcrystalline collagen was prepared from bovine dermal corium; milling was with sharp blades. The preparation consists of partial hydrochloric acid amine collagen salts and retains much of the fibrillar morphology of native collagen.

3-D organization of fibrinogen receptors using computer reconstruction and whole-mount microscopy

1988 ◽  
Vol 46 ◽  
pp. 30-31
Author(s):  
E. T. O'Toole ◽  
R. R. Hantgan ◽  
J. C. Lewis
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Blood Platelets ◽  
Cell Contact ◽  
Computer Assisted ◽  
Adherent Cells ◽  
Differential Centrifugation ◽  
Computer Reconstruction ◽  
Sds Page ◽  
Whole Mount

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.

Abstract #1211 Early Experience of Gene Sequencing Classifier Compared to Gene Expression Classifier

2018 ◽  
Vol 24 ◽  
pp. 309-310
Author(s):  
Mayumi Endo ◽  
Fadi Nabhan ◽  
Laura Ryan ◽  
Shumei Meng ◽  
John Phay ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Sequencing ◽  
Early Experience ◽  
Gene Expression Classifier

Letter: Whole blood volume determinations

1974 ◽  
Vol 134 (1) ◽  
pp. 181b-181
Author(s):  
R. E. Willard
Keyword(s):  
Blood Volume ◽  
Whole Blood

Stability of antioxidant vitamins in whole human blood during overnight storage at 4°C and frozen storage up to 6 months

2018 ◽  
Vol 88 (3-4) ◽  
pp. 151-157 ◽  
Author(s):  
Scott W. Leonard ◽  
Gerd Bobe ◽  
Maret G. Traber
Keyword(s):  
Ascorbic Acid ◽  
Whole Blood ◽  
Healthy Subjects ◽  
Frozen Storage ◽  
Blood Collection ◽  
Plasma Samples ◽  
Optimal Conditions ◽  
Plasma Ascorbic Acid ◽  
Blood Samples ◽  
Sample Handling

Abstract. To determine optimal conditions for blood collection during clinical trials, where sample handling logistics might preclude prompt separation of erythrocytes from plasma, healthy subjects (n=8, 6 M/2F) were recruited and non-fasting blood samples were collected into tubes containing different anticoagulants (ethylenediaminetetra-acetic acid (EDTA), Li-heparin or Na-heparin). We hypothesized that heparin, but not EDTA, would effectively protect plasma tocopherols, ascorbic acid, and vitamin E catabolites (α- and γ-CEHC) from oxidative damage. To test this hypothesis, one set of tubes was processed immediately and plasma samples were stored at −80°C, while the other set was stored at 4°C and processed the following morning (~30 hours) and analyzed, or the samples were analyzed after 6 months of storage. Plasma ascorbic acid, as measured using HPLC with electrochemical detection (LC-ECD) decreased by 75% with overnight storage using EDTA as an anticoagulant, but was unchanged when heparin was used. Neither time prior to processing, nor anticoagulant, had any significant effects upon plasma α- or γ-tocopherols or α- or γ-CEHC concentrations. α- and γ-tocopherol concentrations remained unchanged after 6 months of storage at −80°C, when measured using either LC-ECD or LC/mass spectrometry. Thus, refrigeration of whole blood at 4°C overnight does not change plasma α- or γ-tocopherol concentrations or their catabolites. Ascorbic acid is unstable in whole blood when EDTA is used as an anticoagulant, but when whole blood is collected with heparin, it can be stored overnight and subsequently processed.

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