scholarly journals Gaps in the knowledge of human platelet lysate as a cell culture supplement for cell therapy: a joint publication from the AABB and the International Society for Cell & Gene Therapy

Transfusion ◽  
2019 ◽  
Vol 59 (11) ◽  
pp. 3448-3460 ◽  
Author(s):  
Karen Bieback ◽  
Beatriz Fernandez‐Muñoz ◽  
Shibani Pati ◽  
Richard Schäfer
Keyword(s):  
Gene Therapy ◽  
Cell Culture ◽  
Cell Therapy ◽  
International Society ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Joint Publication ◽  
Human Platelet Lysate ◽  
A Cell ◽  
Cell Gene

Evaluation of different cryoprotective solutions in combination with human platelet lysate: an effective alternative cryoprotectant agent for cell therapy

Cytotherapy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. S190
Author(s):  
C. Rosell-Valle ◽  
M. Martin-Lopez ◽  
M. Montiel ◽  
I. Piudo ◽  
B. Fernandez ◽  
...  
Keyword(s):  
Cell Therapy ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Effective Alternative ◽  
Human Platelet Lysate

Viral safety of human platelet lysate for cell therapy and regenerative medicine: Moving forward, yes, but without forgetting the past

2019 ◽  
Vol 58 (6) ◽  
pp. 102674 ◽  
Author(s):  
Thierry Burnouf ◽  
Lassina Barro ◽  
Ouada Nebie ◽  
Yu-Wen Wu ◽  
Hadi Goubran ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Cell Therapy ◽  
Human Platelet ◽  
Platelet Lysate ◽  
The Past ◽  
Viral Safety ◽  
Human Platelet Lysate

Applications of cook human platelet lysate in cell therapy

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S95
Author(s):  
C.G. Taylor ◽  
R.N. Dayment ◽  
M.Z. Albanna ◽  
E.J. Woods
Keyword(s):  
Cell Therapy ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Human Platelet Lysate

scholarly journals Human Platelet Lysate for Good Manufacturing Practice-Compliant Cell Production

2021 ◽  
Vol 22 (10) ◽  
pp. 5178
Author(s):  
Michaela Oeller ◽  
Sandra Laner-Plamberger ◽  
Linda Krisch ◽  
Eva Rohde ◽  
Dirk Strunk ◽  
...  
Keyword(s):  
Cell Culture ◽  
Blood Donation ◽  
Human Platelet ◽  
Raw Materials ◽  
Cell Types ◽  
Platelet Lysate ◽  
Raw Material ◽  
Specific Cell ◽  
Regulatory Requirements ◽  
Human Platelet Lysate

Numerous cell-based therapeutics are currently being tested in clinical trials. Human platelet lysate (HPL) is a valuable alternative to fetal bovine serum as a cell culture medium supplement for a variety of different cell types. HPL as a raw material permits animal serum-free cell propagation with highly efficient stimulation of cell proliferation, enabling humanized manufacturing of cell therapeutics within a reasonable timeframe. Providers of HPL have to consider dedicated quality issues regarding identity, purity, potency, traceability and safety. Release criteria have to be defined, characterizing the suitability of HPL batches for the support of a specific cell culture. Fresh or expired platelet concentrates from healthy blood donors are the starting material for HPL preparation, according to regulatory requirements. Pooling of individual platelet lysate units into one HPL batch can balance donor variation with regard to essential platelet-derived growth factors and cytokines. The increasingly applied pathogen reduction technologies will further increase HPL safety. In this review article, aspects and regulatory requirements of whole blood donation and details of human platelet lysate manufacturing are presented. International guidelines for raw materials are discussed, and defined quality controls, as well as release criteria for safe and GMP-compliant HPL production, are summarized.

scholarly journals Gelatin-Methacryloyl (GelMA) Formulated with Human Platelet Lysate Supports Mesenchymal Stem Cell Proliferation and Differentiation and Enhances the Hydrogel’s Mechanical Properties

2019 ◽  
Vol 6 (3) ◽  
pp. 76 ◽  
Author(s):  
Marline Kirsch ◽  
Luise Birnstein ◽  
Iliyana Pepelanova ◽  
Wiebke Handke ◽  
Jessica Rach ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Cell Cultures ◽  
Human Platelet ◽  
3D Cell Culture ◽  
Platelet Lysate ◽  
Dependent Manner ◽  
Human Platelet Lysate

Three-dimensional (3D) cell culture is a major focus of current research, since cultivation under physiological conditions provides more reliable information about in vivo cell behavior. 3D cell cultures are used in basic research to better understand intercellular and cell-matrix interactions. However, 3D cell culture plays an increasingly important role in the in vitro testing of bioactive substances and tissue engineering. Gelatin-methacryloyl (GelMA) hydrogels of different degrees of functionalization (DoFs) are a versatile tool for 3D cell culture and related applications such as bioprinting. Human platelet lysate (hPL) has already demonstrated positive effects on 2D cell cultures of different cell types and has proven a valuable alternative to fetal calf serum (FCS). Traditionally, all hydrogels are formulated using buffers. In this study, we supplemented GelMA hydrogels of different DoF with hPL during adipose tissue-derived mesenchymal stem cell (AD-MSCs) encapsulation. We studied the effect of hPL supplementation on the spreading, proliferation, and osteogenic differentiation of AD-MSCs. In addition, the influence of hPL on hydrogel properties was also investigated. We demonstrate that the addition of hPL enhanced AD-MSC spreading, proliferation, and osteogenic differentiation in a concentration-dependent manner. Moreover, the addition of hPL also increased GelMA viscosity and stiffness.

Heparin concentration is critical for cell culture with human platelet lysate

Cytotherapy ◽  
2013 ◽  
Vol 15 (9) ◽  
pp. 1174-1181 ◽  
Author(s):  
Hatim Hemeda ◽  
Jana Kalz ◽  
Gudrun Walenda ◽  
Michael Lohmann ◽  
Wolfgang Wagner
Keyword(s):  
Cell Culture ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Heparin Concentration ◽  
Human Platelet Lysate
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