Noninvasive prenatal paternity testing using targeted massively parallel sequencing

Transfusion ◽  
2018 ◽  
Vol 58 (7) ◽  
pp. 1792-1799 ◽  
Author(s):  
Ning‐ Qu ◽  
Yifan Xie ◽  
Haiyan Li ◽  
Hao‐ Liang ◽  
Shaobin Lin ◽  
...  
Keyword(s):  
Massively Parallel Sequencing ◽  
Paternity Testing ◽  
Massively Parallel ◽  
Parallel Sequencing ◽  
Prenatal Paternity Testing

scholarly journals Noninvasive Prenatal Paternity Testing with a Combination of Well-Established SNP and STR Markers Using Massively Parallel Sequencing

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 454
Author(s):  
Xuefeng Shen ◽  
Ran Li ◽  
Haixia Li ◽  
Yu Gao ◽  
Hui Chen ◽  
...  
Keyword(s):  
Massively Parallel Sequencing ◽  
Maternal Plasma ◽  
Paternity Testing ◽  
Massively Parallel ◽  
Parallel Sequencing ◽  
Str Markers ◽  
Cell Free Fetal Dna ◽  
Paternity Index ◽  
Analytical Strategies ◽  
Prenatal Paternity Testing

Cell-free fetal DNA (cffDNA) from maternal plasma has made it possible to develop noninvasive prenatal paternity testing (NIPPT). However, most studies have focused on customized single nucleotide polymorphism (SNP) typing systems and few have used conventional short tandem repeat (STR) markers. Based on massively parallel sequencing (MPS), this study used a widely-accepted forensic multiplex assay system to evaluate the effect of noninvasive prenatal paternity testing with a combination of well-established SNP and STR markers. Using a ForenSeq DNA Signature Prep Kit, NIPPT was performed in 17 real parentage cases with monovular unborn fetuses at 7 to 24 gestational weeks. Different analytical strategies for the identification of paternally inherited allele (PIA) were developed to deal with SNPs and STRs. Combined paternity index (CPI) for 17 real trios as well as 272 unrelated trios was calculated. With the combination of SNPs and A-STRs, 82.35% (14/17), 88.24% (15/17), 94.12% (16/17), and 94.12% (16/17) of real trios could be accurately determined when the likelihood ratio (LR) threshold for paternity inclusion was set to 10,000, 1000, 100, and 10, respectively. This reveals that simultaneous surveys of SNP and STR markers included in the ForenSeq DNA Signature Prep Kit offer a promising method for NIPPT using MPS technology.

scholarly journals Identification of the Bovine Arachnomelia Mutation by Massively Parallel Sequencing Implicates Sulfite Oxidase (SUOX) in Bone Development

PLoS Genetics ◽  
2010 ◽  
Vol 6 (8) ◽  
pp. e1001079 ◽  
Author(s):  
Cord Drögemüller ◽  
Jens Tetens ◽  
Snaevar Sigurdsson ◽  
Arcangelo Gentile ◽  
Stefania Testoni ◽  
...  
Keyword(s):  
Massively Parallel Sequencing ◽  
Bone Development ◽  
Sulfite Oxidase ◽  
Massively Parallel ◽  
Parallel Sequencing
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