Evaluation of the applicability and effectiveness of a molecular strategy for identifying weak D and DEL phenotype among D- blood donors of mixed origin exhibiting high frequency ofRHD*Ψ

Transfusion ◽  
2017 ◽  
Vol 58 (2) ◽  
pp. 317-322 ◽  
Author(s):  
Marcia Regina Dezan ◽  
Luis Giovani O. Guardalini ◽  
Elaine Pessoa ◽  
Ingrid Helena Ribeiro ◽  
Valeria Brito Oliveira ◽  
...  
Keyword(s):  
High Frequency ◽  
Blood Donors ◽  
Mixed Origin

Cardiovascular risk in 159 934 frequent blood donors while addressing the healthy donor effect

Heart ◽  
2019 ◽  
Vol 105 (16) ◽  
pp. 1260-1265 ◽  
Author(s):  
Karlijn Peffer ◽  
Martin den Heijer ◽  
Wim L A M de Kort ◽  
André L M Verbeek ◽  
Femke Atsma
Keyword(s):  
Cardiovascular Risk ◽  
High Frequency ◽  
Healthy Donor ◽  
Blood Donors ◽  
Cox Regression ◽  
Blood Donation ◽  
Low Frequency ◽  
Sensitivity Analyses ◽  
Hospital Data ◽  
Dutch Hospital

ObjectiveTo investigate whether regular blood donation decreases cardiovascular risk.MethodsAll 159 934 Dutch whole-blood donors with an active donation career of at least 10 years were categorised into sex-specific donation tertiles based on the number of donations during this 10-year qualification period. Cardiovascular endpoints were based on hospital discharge diagnoses and death certificates from Dutch Hospital Data and Statistics Netherlands and occurring after the 10-year qualification period. Cox regression was used to estimate the age-adjusted and starting year-adjusted hazard rate ratio (HRR).ResultsFemale high-frequency blood donors had a reduced cardiovascular morbidity (HRR=0.91, 95% CI 0.85 to 0.98) compared with low-frequency blood donors. No effect was observed in men (HRR=1.00, 95% CI 0.95 to 1.05). To rule out a residual healthy donor effect (HDE), additional sensitivity analyses using a 5-year qualification period were conducted. The results supported the absence of a residual HDE.ConclusionsThis study showed a protective effect of long-term, high-frequency blood donation against cardiovascular disease. This effect was only observed in women and not in men.

scholarly journals FREQUENCY OF DETECTION OF LPS O-ANTIGENS OF DIFFERENT CAUSATIVE AGENTS IN PATIENTS WITH ACUTE DIARRHEAL DISEASES

2017 ◽  
Vol 22 (2) ◽  
pp. 75-79
Author(s):  
O. F Belaia ◽  
O. A Paevskaya ◽  
S. N Zuevskaya ◽  
E. G Korogodskaia ◽  
K. T Umbetova ◽  
...  
Keyword(s):  
High Frequency ◽  
Blood Donors ◽  
Clinical Course ◽  
Control Group ◽  
O Antigen ◽  
Intestinal Infections ◽  
Causative Agents ◽  
Elevated Rate ◽  
O Antigens ◽  
Antigenic Load

The aim is to determine the frequency and dynamics of detection of specific lipopolysaccharide (LPS) O-antigens LPS/O-antigens of causative agents in hospitalized diarrhea patients. Materials and methods. A total of 146 hospitalized diarrhea patients were examined with the use of an immunological method of the reaction of coagglutination (RCA) for the presence of LPS O-antigens of Shigella, Salmonella, Yersinia and Campylobacter in feces as markers of major intestinal infections pathogens. The control group was consisted of 40 blood donors. Results. In acute diarrhea patients there was detected the predominance of Yersinia and Salmonella LPS O-antigens over Shigella and Campylobacter; the high frequency of intestinal bacterial mixt-infection in total (68%), as well as elevated rate incidence of Shigella, Salmonella, Campylobacter, Yersinia antigens in mixt-infections in comparison with those in monoinfections (24%). The total O-antigen "load" in patients with mixt-infection was 3 times higher than in patients with monoinfections; with the increasing of LPS/O-antigen "load" the rate of Salmonella inoculation declines. Conclusion. Under the same severity of the clinical course of the disease, the decline of Salmonella inoculation rate in cases of high antigenic "load" may indicate to the presence of the phenomenon of summation of toxic effects of LPS O-antigens when the concentration of each pathogen in the feces is insufficient (for inoculation) for bacteriological examination.

scholarly journals High frequency of variant RHD genotypes among donors and patients of mixed origin with serologic weak‐D phenotype

2018 ◽  
Vol 32 (9) ◽  
Author(s):  
Marcia Regina Dezan ◽  
Valéria B. Oliveira ◽  
Çarolina Nunes Gomes ◽  
Fabio Luz ◽  
Antônio J. Gallucci ◽  
...  
Keyword(s):  
High Frequency ◽  
Mixed Origin

Immunoblotting with (sub)class-specific antibodies reveals a high frequency of monoclonal gammopathies in persons thought to be immunodeficient.

1988 ◽  
Vol 34 (9) ◽  
pp. 1839-1842 ◽  
Author(s):  
J Radl ◽  
J Wels ◽  
C M Hoogeveen
Keyword(s):  
Elderly Patient ◽  
Monoclonal Antibodies ◽  
Immune System ◽  
High Frequency ◽  
Blood Donors ◽  
Immunosuppressive Treatment ◽  
Dialysis Treatment ◽  
Specific Antibodies ◽  
Monoclonal Gammopathies ◽  
Age Related

Abstract Immunoblotting, in combination with high-resolution electrophoresis and the use of mouse monoclonal antibodies to human (sub)class immunoglobulin (Ig) isotypes, substantially increased the sensitivity with which homogeneous Ig components (H-Ig) could be detected. Using this technique, we reinvestigated 40 selected sera, previously found to be negative for H-Ig by agar electrophoresis and immunofixation, from two groups of individuals thought to have an age-related immunodeficiency, i.e., persons older than 95 years and recipients of kidney grafts who were undergoing immunosuppressive treatment. In both groups, small single or multiple H-Ig components were found, in frequencies of 76% and 79%, respectively. For comparison, the Ig spectrum of 10 sera from patients on dialysis treatment and of 33 sera from young adult blood donors was ordinarily heterogeneous, except for one elderly patient and one blood donor with a previously unknown IgG2 deficiency. These results are complementary to the observations in some immunodeficiencies in children and indicate that the appearance of single or multiple H-Ig components in low concentration can be considered a very sensitive indicator of certain immune system disorders forming a separate category of monoclonal gammopathies.

scholarly journals Transfusion management of patients with red blood cell antibodies

2013 ◽  
Vol 66 (11-12) ◽  
pp. 491-496 ◽  
Author(s):  
Nevenka Bujandric ◽  
Jasmina Grujic ◽  
Mirjana Krga-Milanovic
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
High Frequency ◽  
Blood Donors ◽  
Blood Compatibility ◽  
Blood Group System ◽  
System 2 ◽  
Special Challenge ◽  
Clinically Significant ◽  
D 12

Introduction. Red blood cell antibodies may cause a positive result of pre-transfusion blood compatibility testing (crossmatch test). It can be a problem to provide suitable blood units for patients with clinically significant antibodies to high-frequency antigens as well as for those with multiple alloantibody specificities. This study was aimed at identifying transfused patients in the population of South-Backa who had developed clinically significant red blood cell alloantibodies. Material and methods. We analyzed the records of crossmatch results and antibody screening performed at the Blood Transfusion Institute of Vojvodina during 2012. Results. Antibodies were found in 103 patients: A) 63 patients with single antibodies: 1) 16 with antibodies of unknown specificity (3 autoantibodies, 13 alloantibodies); 2) 39 with clinically significant antibodies (23 from Rh system (2 anti-C, 2 anti-D, 12 anti-E, 7 anti-c), 4 anti-K, 3 anti-Fya, 7 anti-Jka, 2 anti-S); 3) 8 with usually not significant antibodies (6 anti-M, 1 anti-A1, 1 anti- Cw); B) 40 patients developed multiple antibodies: 1) all patients had at least one clinically significant antibody from various blood group system (44 Rh, 13 Kell, 7 Kidd, 7 MNSs (S, s)); 2) 3 patients had usually not significant antibodies (1 Lewis, 2 Lutheran); 3) 3 patients occasionally had clinically significant antibody (3 anti- Yta); 4) 3 patients had antibodies of unknown specificity (2 autoantibodies, 1alloantibody). Antibodies detected in the majority of patients (65-63.1%) had a specificity of Rh and/or the Kell system. Conclusions. The main goal of pre-transfusion blood compatibility testing is to detect clinically significant antibodies. The provision of antigen negative blood units for those patients is a special challenge for blood establishments. Database with a sufficient number of typed blood donors can help to resolve this problem.

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