scholarly journals Collaborative study to establish World Health Organization international reference reagents for dengue virus Types 1 to 4 RNA for use in nucleic acid testing

Transfusion ◽  
2017 ◽  
Vol 57 (8) ◽  
pp. 1977-1987 ◽  
Author(s):  
Germán Añez ◽  
Evgeniya Volkova ◽  
Zhen Jiang ◽  
Daniel A. R. Heisey ◽  
Caren Chancey ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dengue Virus ◽  
World Health Organization ◽  
Collaborative Study ◽  
World Health ◽  
Nucleic Acid Testing ◽  
International Reference ◽  
Health Organization

Establishment of the second international standards for porcine and human calcitonins: report of the international collaborative study

1993 ◽  
Vol 128 (5) ◽  
pp. 443-450 ◽  
Author(s):  
Joan M Zanelli ◽  
Rose E Gaines-Das ◽  
P Corran
Keyword(s):  
World Health Organization ◽  
Collaborative Study ◽  
International Standards ◽  
World Health ◽  
International Reference ◽  
International Standard ◽  
Reference Preparation ◽  
Health Organization ◽  
International Collaborative Study

The biological potency of calcitonins in clinical use in long-term treatment of Paget's disease of bone and, increasingly, in osteoporosis is usually expressed in international units defined by the relevant World Health Organization international reference preparation. The international reference preparations for porcine and human calcitonins were ampouled in 1970 and stocks are now exhausted. Replacement standards were ampouled in 1989 and have been evaluated and calibrated by an international collaborative study comprising 16 laboratories in 12 countries. Evaluations included high-performance liquid chromatography and in vitro bioassay; calibration of each new ampouled preparation in terms of its international reference preparation was by in vivo rat hypocalcaemia bioassay. On the basis of the results of the study and with the agreement of the participants, replacement standards were established by the Expert Committee on Biological Standardization of the World Health Organization in 1991: the international standard for porcine calcitonin (ampoule code 89/540), with an assigned potency of 0.8 international units per ampoule, and the international standard for human calcitonin, with an assigned potency of 17.5 international units per ampoule. Both international standards appeared to be sufficiently stable to serve as the international standards for in vivo biological assays. Comparison of the two species of calcitonin in the same hypocalcaemia assay showed that they were approximately equipotent when the doses were given intravenously but that the human peptide was four- to sixfold more potent than porcine calcitonin when doses were given subcutaneously, emphasizing the need to compare "like with like".

scholarly journals Harmonization of nucleic acid testing for Zika virus: development of the 1st World Health Organization International Standard

Transfusion ◽  
2017 ◽  
Vol 57 (3pt2) ◽  
pp. 748-761 ◽  
Author(s):  
Sally A. Baylis ◽  
Kay-Martin O. Hanschmann ◽  
Barbara S. Schnierle ◽  
Jan-Hendrik Trösemeier ◽  
Johannes Blümel ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
World Health Organization ◽  
Zika Virus ◽  
World Health ◽  
Nucleic Acid Testing ◽  
International Standard ◽  
Health Organization

scholarly journals A World Health Organization Human Hepatitis E Virus Reference Strain Related to Similar Strains Isolated from Rabbits

2018 ◽  
Vol 6 (16) ◽  
pp. e00292-18 ◽  
Author(s):  
Marco Kaiser ◽  
Déborah Delaune ◽  
Olivier Chazouillères ◽  
Johannes Blümel ◽  
Anne-Marie Roque-Afonso ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sequence Analysis ◽  
World Health Organization ◽  
Hepatitis E Virus ◽  
Reference Strain ◽  
Hepatitis E ◽  
World Health ◽  
Nucleic Acid Testing ◽  
Full Length Sequence ◽  
Health Organization

ABSTRACT We report here the genome sequence of a hepatitis E virus (HEV) strain from a chronically infected immunodeficient patient. Full-length sequence analysis revealed a distinct HEV strain, of a tentative new subgenotype, clustering with viruses from rabbits. It is a World Health Organization reference strain for validation of nucleic acid testing.

scholarly journals Genome Sequence of a Candidate World Health Organization Reference Strain of Zika Virus for Nucleic Acid Testing

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Jan-Hendrik Trösemeier ◽  
Didier Musso ◽  
Johannes Blümel ◽  
Julien Thézé ◽  
Oliver G. Pybus ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
World Health Organization ◽  
Zika Virus ◽  
Reference Strain ◽  
Nucleic Acid Amplification ◽  
World Health ◽  
Nucleic Acid Testing ◽  
The World ◽  
Health Organization ◽  
Detection And Quantification

We report here the sequence of a candidate reference strain of Zika virus (ZIKV) developed on behalf of the World Health Organization (WHO). The ZIKV reference strain is intended for use in nucleic acid amplification (NAT)-based assays for the detection and quantification of ZIKV RNA.

Sign in / Sign up

Export Citation Format

Share Document